FabHA - Revision history

Jstuelk: /* Original Publications */

2014-09-19T10:58:55Z

‎Original Publications

134.76.70.252 at 14:06, 17 April 2014

2014-04-17T14:06:11Z

134.76.70.252 at 09:28, 17 April 2014

2014-04-17T09:28:22Z

134.76.38.147 at 13:24, 2 April 2014

2014-04-02T13:24:23Z

Jstuelk at 15:30, 18 March 2014

2014-03-18T15:30:11Z

134.76.70.252 at 10:30, 7 January 2014

2014-01-07T10:30:38Z

134.76.70.252 at 12:58, 16 May 2013

2013-05-16T12:58:12Z

134.76.70.252 at 09:54, 14 May 2013

2013-05-14T09:54:11Z

134.76.70.252 at 12:32, 13 May 2013

2013-05-13T12:32:12Z

Raphael2215 at 11:14, 9 August 2012

2012-08-09T11:14:22Z

← Older revision		Revision as of 10:58, 19 September 2014
Line 1:		Line 1:
−	* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed ''B. subtilis'' cells <br/><br/>	+	{{#bank: FabHA}}
−	{~~\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''fabHA''~~
−	\|-
−	~~\|style="background:~~#~~ABCDEF;" align="center"\| '''Synonyms''' \|\| ''yjaX '', ''fabH1 ''~~
−	\|-
−	~~\|style="background~~:~~#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| beta-ketoacyl-acyl carrier protein synthase III~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| fatty acid biosynthesis~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11330 fabHA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=fabHA fabHA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 33 kDa, 5.045~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 936 bp, 312 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[yjzB]]'', ''[[fabF]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11330 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:fabHA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	~~\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fabHA_1208222_1209160_1 Expression at a glance]'''   {{PubMed\|22383849~~}~~}<br/>[[Image:fabHA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11330]]~~
−	\|-
−	\|}

−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[biosynthesis of lipids]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[FapR regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU11330
−
−	~~===Phenotypes of a mutant ===~~
−	* significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species {{PubMed\|21542858}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11330&redirect=T BSU11330]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13127]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO<sub>2</sub> (according to Swiss-Prot)
−
−	* '''Protein family:''' fabH family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):''' [[FabHB]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:'''
−	** cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11330&redirect=T BSU11330]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed\|10673437}}
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/O34746 O34746]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11330]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.180 2.3.1.180], [http://www.expasy.org/enzyme/2.3.1.41 2.3.1.41]
−
−	~~=== Additional information===~~
−	* affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis {{PubMed\|19820084}}
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed\|12737802}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fabHA_1208222_1209160_1 fabHA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|12737802}}
−
−	* '''Regulation:'''
−	** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed\|12737802}}
−	** inhibited by cerulenin {{PubMed\|11325930}}
−	** induced upon fatty acid biosynthesis inhibition {{PubMed\|21383089}}
−	** expression is reduced when [[SigW]] is activated (by alkaline shock, polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100) {{PubMed\|21542858}}
−
−	* '''Regulatory mechanism:'''
−	** [[FapR]]: transcription repression {{PubMed\|12737802}}
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 897 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 1078 {{PubMed\|24696501}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 2129 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1833 {{PubMed\|21395229}}
−	** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2586 {{PubMed\|21395229}}
−
−	~~=Biological materials =~~
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15952903 17919287</pubmed>~~
−	~~==Original Publications==~~
−	~~<pubmed>12737802,17114254,10629181, 10673437,19820084 21383089 21542858</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 125: / Line 125: @@
 ** number of protein molecules per cell (minimal medium with glucose and ammonium): 897 {{PubMed|24696501}}
 ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1078 {{PubMed|24696501}}
 =Biological materials =
 * '''Mutant:'''

← Older revision		Revision as of 09:28, 17 April 2014
Line 123:		Line 123:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 897 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 1078 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]
@@ Line 91: / Line 92: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}

@@ Line 38: / Line 38: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
-<br/><br/><br/><br/>
+<br/><br/>
 = [[Categories]] containing this gene/protein =
@@ Line 66: / Line 62: @@
 === Additional information===
 =The protein=
@@ Line 82: / Line 77: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 114: / Line 109: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fabHA_1208222_1209160_1 fabHA] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigA]] {{PubMed|12737802}}
+* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12737802}}
 * '''Regulation:'''
@@ Line 149: / Line 144: @@
 <pubmed> 15952903 17919287</pubmed>
 ==Original Publications==
-'''Additional publications:'''  {{PubMed|21542858}}
+<pubmed>12737802,17114254,10629181, 10673437,19820084 21383089 21542858</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 15: / Line 15: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11330 fabHA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=fabHA fabHA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.045

@@ Line 30: / Line 30: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fabHA_1208222_1209160_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fabHA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fabHA_1208222_1209160_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fabHA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11330]]
 |-
 |}

@@ Line 23: / Line 23: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjzB]]'', ''[[fabF]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11330 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11330 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11330 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11330 DNA_with_flanks]
 |-
 |-

@@ Line 1: / Line 1: @@
+* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed ''B. subtilis'' cells <br/><br/>
 {| align="right" border="1" cellpadding="2"
 |-