EngA - Revision history

134.76.70.252 at 14:20, 17 April 2014

2014-04-17T14:20:01Z

134.76.70.252 at 11:25, 17 April 2014

2014-04-17T11:25:28Z

134.76.38.147 at 14:04, 2 April 2014

2014-04-02T14:04:37Z

134.76.70.252 at 13:46, 16 May 2013

2013-05-16T13:46:34Z

134.76.70.252 at 10:35, 14 May 2013

2013-05-14T10:35:15Z

134.76.70.252 at 13:10, 13 May 2013

2013-05-13T13:10:00Z

Awiechert at 12:48, 3 December 2012

2012-12-03T12:48:51Z

Jstuelk at 18:57, 14 October 2012

2012-10-14T18:57:57Z

Jstuelk: moved YphC to EngA over redirect: 23056455

2012-10-14T18:48:51Z

moved YphC to EngA over redirect: 23056455

134.76.70.252 at 11:08, 7 August 2012

2012-08-07T11:08:32Z

← Older revision		Revision as of 14:20, 17 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' GTPase essential for ribosome 50S subunit assembly<br/><br/>~~	+	{{#bank: EngA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''yphC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' engA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| GTPase~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| ribosome assembly~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840 engA]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 48 kDa, 5.248~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1308 bp, 436 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[gpsA]]'', ''[[ypzH]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22840 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:yphC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:yphC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[translation]]}},~~
−	~~{{SubtiWiki category\|[[essential genes]]}},~~
−	~~{{SubtiWiki category\|[[GTP-binding proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU22840
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22840&redirect=T BSU22840]
−
−	* '''DBTBS entry:''' no entry
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11443]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
−
−	* '''Protein family:''' EngA subfamily (according to Swiss-Prot) Era/Obg family
−
−	* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−
−	* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22840&redirect=T BSU22840]
−
−	* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2HJG 2HJG] (complex with GDP)
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P50743 P50743]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22840]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:'''
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 engA] {{PubMed\|22383849}}
−
−	* '''Sigma factor:'''
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−
−	* '''Additional information:'''
−	** number of protein molecules per cell (minimal medium with glucose and ammonium): 225 {{PubMed\|24696501}}
−	** number of protein molecules per cell (complex medium with amino acids, without glucose): 935 {{PubMed\|24696501}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−	** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846, available in [[Jörg Stülke]]'s lab
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~[[Naotake Ogasawara]], Nara, Japan~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 19575570 21885683</pubmed>~~
−
−	~~==Original publications==~~
−	~~<pubmed>12427945, 16997968,16894162 16682769 23056455</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

← Older revision		Revision as of 11:25, 17 April 2014
Line 119:		Line 119:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 225 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 935 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' no entry
@@ Line 93: / Line 94: @@
 === Database entries ===
 * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2HJG 2HJG] (complex with GDP)

@@ Line 27: / Line 27: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yphC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yphC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840]]
 |-
 |}

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gpsA]]'', ''[[ypzH]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22840 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22840 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yphC_context.gif]]

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22840 engA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22840 engA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.248
@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gpsA]]'', ''[[ypzH]]''
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14200&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22840 Advanced_DNA]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:yphC_context.gif]]

@@ Line 20: / Line 20: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1308 bp, 436 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gpsA]]'', ''[[seaA]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gpsA]]'', ''[[ypzH]]''
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14200&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

@@ Line 14: / Line 14: @@
 |style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22840 yphC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22840 engA]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.248
@@ Line 35: / Line 35: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 78: / Line 74: @@
 * '''Protein family:''' EngA subfamily (according to Swiss-Prot) Era/Obg family
-* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YphC]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
+* '''Paralogous protein(s):''' [[Era]], [[Obg]], [[YsxC]], [[RbgA]], [[YqeH]], [[ThdF]], [[YyaF]], [[YnbA]]
 === Extended information on the protein ===
@@ Line 112: / Line 108: @@
 * '''Operon:'''
-* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 yphC] {{PubMed|22383849}}
+* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=engA_2390206_2391516_-1 engA] {{PubMed|22383849}}
 * '''Sigma factor:'''
@@ Line 127: / Line 123: @@
 * '''Expression vector:'''
-** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846,  available in [[Stülke]] lab
+** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP846,  available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''
@@ Line 148: / Line 144: @@
 ==Original publications==
-<pubmed>12427945, 16997968,16894162 16682769  </pubmed>
+<pubmed>12427945, 16997968,16894162 16682769 23056455</pubmed>
 [[Category:Protein-coding genes]]

← Older revision	Revision as of 18:48, 14 October 2012
(No difference)