DivIVA - Revision history

Jstuelk at 15:54, 10 April 2015

2015-04-10T15:54:52Z

2015-03-06T13:17:38Z

‎Biological materials

2015-03-06T13:14:13Z

‎Biological materials

2015-03-06T10:25:53Z

‎Biological materials

2014-11-18T13:51:01Z

‎Reviews

2014-08-11T09:00:25Z

‎The protein

2014-08-11T08:56:42Z

2014-04-17T09:37:06Z

2014-04-05T18:51:59Z

‎Biological materials

2014-04-02T13:40:16Z

@@ Line 154: / Line 154: @@
 * '''Expression vector:'''
 ** DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]
-** pGP1497 (N-terminal Strep-tag fused to C-terminus of ''divIVA'', TEV-site purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
+** pGP1497 (N-terminal Strep-tag fused to C-terminus of ''divIVA'', TEV-site, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
 * '''lacZ fusion:'''

@@ Line 152: / Line 152: @@
 ** GP1482 (chromosomal ''divIVA''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab'''
-* '''Expression vector:''' DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]
+* '''Expression vector:'''
+** DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]
 * '''lacZ fusion:'''

@@ Line 150: / Line 150: @@
 * '''Mutant:'''
 ** 4041 (''divIVA''::''tet''), available in [[Leendert Hamoen]]'s, [[Jörg Stülke]]'s, and [[Sven Halbedel]] 's lab
 * '''Expression vector:''' DivIVA-Strep available [http://www.rki.de/DE/Content/Institut/OrgEinheiten/Abt1/FG11/AG_LM.html here]

@@ Line 173: / Line 173: @@
 =References=
 ==Reviews==
-<pubmed> 19654604, 19884039 22722244 </pubmed>
+<pubmed> 19654604, 19884039 22722244 23182676 </pubmed>
 ==Original Publications==
 <pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 24391905 24592260 24600441 25101664 </pubmed>
 [[Category:Protein-coding genes]]

@@ Line 76: / Line 76: @@
 ** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].
 ** DivIVA may anchor [[SpoIIE]] briefly to the assembling polar septum before [[SpoIIE]] is subsequently released into the forespore membrane and recaptured at the polar septum {{PubMed|25101664}}
 * '''Protein family:''' gpsB family (according to Swiss-Prot)
@@ Line 112: / Line 113: @@
 ** forms rings at the division septum and patches at the cell poles {{PubMed|22108385}}
 ** membrane targeting requires [[SecA]] {{PubMed|24592260}}
+** assembles into a ring-like structure at the polar septum during [[sporulation]] {{PubMed|25101664}}
 === Database entries ===
 * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15420&redirect=T BSU15420]

@@ Line 75: / Line 75: @@
 ** DivIVA is required for polar localisation of [[MinC]]-[[MinD]] via [[MinJ]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19019154 PubMed]
 ** It also recruits [[RacA]] to the distal pole of the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/12493822 PubMed].
 * '''Protein family:''' gpsB family (according to Swiss-Prot)
@@ Line 105: / Line 106: @@
 ** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}
 ** [[DivIVA]]-[[SecA]] {{PubMed|24592260}}
 * '''[[Localization]]:'''
@@ Line 173: / Line 175: @@
 ==Original Publications==
-<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 24391905 24592260 24600441 </pubmed>
+<pubmed>22582279, 19654604, 19666580,9219999,19019154,15554965, 12368265,11445541,10835369,12511520,14651647, 19478798 ,19429628, 11445541, 9219999, 9045828 20352045 20502438 11886553 21564336 22108385 22457634 22517742 22661688 23264578 23701187 23927765 24391905 24592260 24600441 25101664 </pubmed>
 [[Category:Protein-coding genes]]

← Older revision		Revision as of 09:37, 17 April 2014
Line 140:		Line 140:

	* '''Additional information:'''		* '''Additional information:'''
		+	** number of protein molecules per cell (minimal medium with glucose and ammonium): 1595 {{PubMed\|24696501}}
		+	** number of protein molecules per cell (complex medium with amino acids, without glucose): 3553 {{PubMed\|24696501}}

	=Biological materials =		=Biological materials =

@@ Line 154: / Line 154: @@
 * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Sven Halbedel]]'s and [[Jörg Stülke]]'s labs
-* '''Antibody:'''
+* '''Antibody:''' A polyclonal anti-DivIVA antiserum generated in rabbit is described here {{PubMed|11445541}}.
 =Labs working on this gene/protein=

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/divIVA.html]
@@ Line 111: / Line 112: @@
 === Database entries ===
 * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2WUJ 2WUJ] (N-terminal domain) {{PubMed|20502438}}