CotC - Revision history

Jstuelk: /* Expression and regulation */

2015-04-06T13:39:35Z

‎Expression and regulation

134.76.38.147 at 13:49, 2 April 2014

2014-04-02T13:49:36Z

134.76.70.252 at 08:30, 12 November 2013

2013-11-12T08:30:26Z

134.76.70.252 at 13:28, 16 May 2013

2013-05-16T13:28:14Z

134.76.70.252 at 10:20, 14 May 2013

2013-05-14T10:20:08Z

134.76.70.252 at 12:55, 13 May 2013

2013-05-13T12:55:48Z

Jstuelk: /* References */

2013-01-25T17:43:45Z

‎References

Jstuelk at 17:42, 25 January 2013

2013-01-25T17:42:15Z

Awiechert at 10:18, 3 December 2012

2012-12-03T10:18:43Z

134.76.70.252 at 09:02, 19 September 2012

2012-09-19T09:02:29Z

← Older revision		Revision as of 13:39, 6 April 2015
Line 1:		Line 1:
−	* '''Description:~~''' spore coat protein (outer) <br/><br/>~~	+	{{#bank: CotC}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''cotC''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| spore coat protein (outer)~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| resistance of the spore~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17700 cotC]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CotC CotC]
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 14 kDa, 9.558~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 354 bp, 118 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[ynzK]]'', ''[[tatAC]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17700 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:cotC_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotC_1904995_1905195_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:cotC_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17700]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[sporulation proteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[GerE regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SigK regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[SpoIIID regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU17700
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17700&redirect=T BSU17700]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cotC.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10492]
−
−	~~=== Additional information===~~
−
−
−
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:'''
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[CotC]]-[[CotU]] {{PubMed\|18065538}}
−	** [[CotE]]-[[CotC]] {{PubMed\|20023017}}
−
−	* '''[[Localization]]:'''
−	** outer spore coat, more abundant at the mother cell-distal pole of the forespore {{PubMed\|19933362}}
−	** the [[CotC]]-[[CotU]] complex assembles around the forming spore {{PubMed\|18065538}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17700&redirect=T BSU17700]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P07790 P07790]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17700]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''cotC'' (according to [http://dbtbs.hgc.jp/COG/prom/cotC.html DBTBS])
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotC_1904995_1905195_-1 cotC] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigK]] {{PubMed\|1518043}}
−
−	* '''Regulation:'''
−	** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]], [[SpoIIID]]) {{PubMed\|1518043,10788508}}
−
−	* '''Regulatory mechanism:'''
−	** [[GerE]]: transcription activation {{PubMed\|1518043}}
−	** [[SpoIIID]]: transcription repression {{PubMed\|10788508}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−	** 1S103 ( ''cotC''::''cat''), {{PubMed\|2821284}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S103&Search=1S103 BGSC]
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−
−	~~<pubmed> 19933362,1518043,2821284,12031481,1691789,18065538 10788508 20023017 15470121 </pubmed>~~
−	~~==Application of CotZ for protein surface display==~~
−	~~'''Additional publications:''' {{PubMed\|23334774}}~~
−	~~<pubmed> 17373477</pubmed>~~
−	~~[[Category:Protein-coding genes]]~~

@@ Line 56: / Line 56: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cotC.html]
@@ Line 97: / Line 98: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17700 cotC]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CotC CotC]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CotC CotC]
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 9.558

@@ Line 29: / Line 29: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotC_1904995_1905195_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cotC_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotC_1904995_1905195_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cotC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17700]]
 |-
 |}

@@ Line 24: / Line 24: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzK]]'', ''[[tatAC]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17700 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17700 DNA_with_flanks]
 |-
 |colspan="2" | '''Genetic context''' <br/> [[Image:cotC_context.gif]]

@@ Line 146: / Line 146: @@
 =References=
-<pubmed> 19933362,1518043,2821284,12031481,1691789,18065538 10788508  20023017 15470121 17373477 </pubmed>
+<pubmed> 19933362,1518043,2821284,12031481,1691789,18065538 10788508  20023017 15470121 </pubmed>
 ==Application of CotZ for protein surface display==
-<pubmed> 23334774 </pubmed>
+'''Additional publications:''' {{PubMed|23334774}}
 [[Category:Protein-coding genes]]

@@ Line 37: / Line 37: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 118: / Line 114: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotC_1904995_1905195_-1 cotC] {{PubMed|22383849}}
-* '''Sigma factor:''' [[SigK]] {{PubMed|1518043}}
+* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|1518043}}
 * '''Regulation:'''
@@ Line 151: / Line 147: @@
 <pubmed> 19933362,1518043,2821284,12031481,1691789,18065538 10788508  20023017 15470121 17373477 </pubmed>
+==Application of CotZ for protein surface display==
 [[Category:Protein-coding genes]]

@@ Line 22: / Line 22: @@
 |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 354 bp, 118 aa
 |-
-|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yncM]]'', ''[[tatAC]]''
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzK]]'', ''[[tatAC]]''
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13654&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''

← Older revision		Revision as of 09:02, 19 September 2012
Line 132:		Line 132:

	* '''Mutant:'''		* '''Mutant:'''
		+	** 1S103 ( ''cotC''::''cat''), {{PubMed\|2821284}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S103&Search=1S103 BGSC]

	* '''Expression vector:'''		* '''Expression vector:'''