ComFA - Revision history

Jstuelk: /* Original publications */

2015-05-28T15:30:35Z

‎Original publications

Dzeigler: /* Phenotypes of a mutant */

2015-05-28T15:27:54Z

‎Phenotypes of a mutant

Dzeigler: /* Phenotypes of a mutant */

2015-05-28T15:25:41Z

‎Phenotypes of a mutant

Dzeigler: /* Phenotypes of a mutant */

2015-05-28T15:24:14Z

‎Phenotypes of a mutant

Jstuelk at 14:35, 8 April 2014

2014-04-08T14:35:23Z

134.76.70.252 at 11:45, 8 April 2014

2014-04-08T11:45:53Z

134.76.38.147 at 14:56, 2 April 2014

2014-04-02T14:56:15Z

Jstuelk at 11:17, 16 March 2014

2014-03-16T11:17:11Z

134.76.70.252 at 09:28, 12 November 2013

2013-11-12T09:28:02Z

134.76.70.252 at 14:42, 16 May 2013

2013-05-16T14:42:39Z

← Older revision		Revision as of 15:30, 28 May 2015
Line 1:		Line 1:
−	* '''Description:~~''' membrane-associated ATPase, may provide energy for DNA transport<br/><br/>~~	+	{{#bank: ComFA}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''comFA''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| '' ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| ATP-binding protein~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| [[genetic competence]]~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35470 comFA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComFA ComFA]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ComFA ComFA]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 52 kDa, 9.824~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1389 bp, 463 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[comFB]]'', ''[[yviA]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35470 DNA_with_flanks]
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:comFA_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:comFA_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35470]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[genetic competence]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[ComK regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU35470
−
−	~~===Phenotypes of a mutant ===~~
−	* knockout has 1000-fold reduction in transformability compared to the wild-type [http://www.ncbi.nlm.nih.gov/pubmed/8412657 PubMed]
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35470&redirect=T BSU35470]
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comFABC-yvyF-flgM-yvyG-flgKL.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10395]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:'''
−
−	* '''Protein family:''' helicase family (according to Swiss-Prot)
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''[[Domains]]:'''
−
−	* '''Modification:'''
−	** phosphorylated on Arg-20, Arg-186, and Arg-446 {{PubMed\|22517742}}
−
−	* '''[[Cofactors]]:'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[ComGA]]-[[ComFA]] {{PubMed\|17630974}}
−	** [[ComEC]]-[[ComFA]] {{PubMed\|17630974}}
−	** [[CoiA]]-[[ComFA]] {{PubMed\|17630974}}
−	** [[SsbB]]-[[ComFA]] {{PubMed\|17630974}}
−	** [[DprA]]-[[ComFA]] {{PubMed\|17630974}}
−	** [[RecA]]-[[ComFA]] {{PubMed\|17630974}}
−
−	* '''[[Localization]]:'''
−	** cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/7984101 PubMed]
−	** localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
−	** localizes to cell poles {{PubMed\|21278288}}
−
−	~~=== Database entries ===~~
−	* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35470&redirect=T BSU35470]
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P39145 P39145]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35470]
−
−	* '''E.C. number:'''
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−	* '''Operon:''' ''[[comFA]]-[[comFB]]-[[comFC]]-[[yvyF]]-[[flgM]]-[[flgN]]-[[flgK]]-[[flgL]]'' {{PubMed\|8412657}}
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 comFA] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' ''[[comFA]]'': [[SigA]] {{PubMed\|8412657}}
−
−	* '''Regulation:'''
−
−	* '''Regulatory mechanism:'''
−	** [[ComK]]: transcription activation {{PubMed\|8412657}}
−
−	* '''Additional information:'''
−
−	~~=Biological materials =~~
−
−	* '''Mutant:'''
−
−	* '''Expression vector:'''
−
−	* '''lacZ fusion:'''
−
−	* '''GFP fusion:'''
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~==Reviews==~~
−	~~<pubmed> 15083159 </pubmed>~~
−	~~==Original publications==~~
−	~~'''Additional publications:''' {{PubMed\|21397556}}~~
−	~~<pubmed>11814663,8412657,7984101,16009133,8045895,17630974, 21278288 22517742</pubmed>~~
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 56: / Line 56: @@
 ===Phenotypes of a mutant ===
-knockout has 1000-fold reduction in transformability compared to the wild-type [http://www.ncbi.nlm.nih.gov/pubmed/8412657 PubMed]
+* knockout has 1000-fold reduction in transformability compared to the wild-type [http://www.ncbi.nlm.nih.gov/pubmed/8412657 PubMed]
 === Database entries ===

@@ Line 56: / Line 56: @@
 ===Phenotypes of a mutant ===
-knockout has 1000-fold reduction in transformability compared to the wild-type
+knockout has 1000-fold reduction in transformability compared to the wild-type [http://www.ncbi.nlm.nih.gov/pubmed/8412657 PubMed]
 === Database entries ===

← Older revision		Revision as of 15:24, 28 May 2015
Line 56:		Line 56:

	===Phenotypes of a mutant ===		===Phenotypes of a mutant ===
		+	knockout has 1000-fold reduction in transformability compared to the wild-type

	=== Database entries ===		=== Database entries ===

@@ Line 65: / Line 65: @@
 === Additional information===
 =The protein=
@@ Line 117: / Line 116: @@
 =Expression and regulation=
-* '''Operon:''' ''[[comFA]]-[[comFB]]-[[comFC]]-[[yvyF]]-[[flgM]]-[[yvyG]]-[[flgK]]-[[flgL]]'' {{PubMed|8412657}}
+* '''Operon:''' ''[[comFA]]-[[comFB]]-[[comFC]]-[[yvyF]]-[[flgM]]-[[flgN]]-[[flgK]]-[[flgL]]'' {{PubMed|8412657}}
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 comFA] {{PubMed|22383849}}

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComFA ComFA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in  [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ComFA ComFA]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 9.824

@@ Line 58: / Line 58: @@
 === Database entries ===
 * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comFABC-yvyF-flgM-yvyG-flgKL.html]
@@ Line 103: / Line 104: @@
 === Database entries ===
 * '''Structure:'''

@@ Line 1: / Line 1: @@
-* '''Description:''' late competence protein required for DNA binding and uptake <br/><br/>
+* '''Description:''' membrane-associated ATPase, may provide energy for DNA transport<br/><br/>
 {| align="right" border="1" cellpadding="2"
@@ Line 39: / Line 39: @@
 <br/><br/><br/><br/>
 <br/><br/><br/><br/>
 <br/><br/>
@@ Line 84: / Line 80: @@
 * '''Kinetic information:'''
-* '''Domains:'''
+* '''[[Domains]]:'''
 * '''Modification:'''
 ** phosphorylated on Arg-20, Arg-186, and Arg-446 {{PubMed|22517742}}
-* '''Cofactor(s):'''
+* '''[[Cofactors]]:'''
 * '''Effectors of protein activity:'''
@@ Line 123: / Line 119: @@
 * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 comFA] {{PubMed|22383849}}
-* '''Sigma factor:'''  ''[[comFA]]'': [[SigA]] {{PubMed|8412657}}
+* '''[[Sigma factor]]:'''  ''[[comFA]]'': [[SigA]] {{PubMed|8412657}}
 * '''Regulation:'''

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35470 comFA]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ComFA ComFA]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComFA ComFA]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''

@@ Line 31: / Line 31: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:comFA_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comFA_3642167_3643558_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:comFA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35470]]
 |-
 |}