BglP - Revision history

134.76.38.147 at 15:11, 2 April 2014

2014-04-02T15:11:36Z

134.76.70.252 at 12:00, 7 January 2014

2014-01-07T12:00:22Z

134.76.70.252 at 09:43, 12 November 2013

2013-11-12T09:43:03Z

134.76.70.252 at 14:57, 16 May 2013

2013-05-16T14:57:44Z

134.76.70.252 at 11:39, 14 May 2013

2013-05-14T11:39:56Z

134.76.70.252 at 14:14, 13 May 2013

2013-05-13T14:14:28Z

Jstuelk: /* References */

2013-03-27T10:19:19Z

‎References

Jstuelk: /* References */

2013-03-14T09:52:43Z

‎References

Jstuelk: /* References */

2013-03-14T09:51:37Z

‎References

Jstuelk: /* References */

2013-03-14T09:50:38Z

‎References

← Older revision		Revision as of 15:11, 2 April 2014
Line 1:		Line 1:
−	* '''Description:~~''' [[trigger enzymes\|trigger enzyme]]: beta-glucoside-specific [[PTS\|phosphotransferase system]], EIIBCA of the [[PTS]]<br/><br/>~~	+	{{#bank: BglP}}

−	~~{\| align="right" border="1" cellpadding="2"~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Gene name'''~~
−	~~\|''bglP''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Synonyms''' \|\| ''sytA ''~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Essential''' \|\| no~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Product''' \|\| [[trigger enzymes\|trigger enzyme]]: beta-glucoside-specific <br/>[[PTS\|phosphotransferase system]], EIIBCA~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Function''' \|\| beta-glucoside uptake and phosphorylation, <br/>control of [[LicT]] activity~~
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39270 bglP]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=BglP BglP]
−	\|-
−	\|colspan="2" style="background:#FAF8CC;" align="center"\| '''Metabolic function and regulation of this protein in [[SubtiPathways\|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bglP bglP]'''
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''MW, pI''' \|\| 64 kDa, 6.809~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\| '''Gene length, protein length''' \|\| 1827 bp, 609 aa~~
−	\|-
−	~~\|style="background:#ABCDEF;" align="center"\|'''Immediate neighbours''' \|\| ''[[bglH]]'', ''[[yxxE]]''~~
−	\|-
−	\|style="background:#FAF8CC;" align="center"\|'''Sequences'''\|\|[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39270 DNA_with_flanks]
−	\|-
−	\|-
−	\|-
−	~~\|colspan="2" \| '''Genetic context''' <br/> [[Image:bglP_context.gif]]~~
−	~~<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>~~
−	\|-
−	\|colspan="2" \|'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''   {{PubMed\|22383849}}<br/>[[Image:bglP_expression.png\|500px\|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39270]]
−	\|-
−	\|}
−
−	~~__TOC__~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/><br/><br/>~~
−	~~<br/><br/>~~
−
−	~~= [[Categories]] containing this gene/protein =~~
−	~~{{SubtiWiki category\|[[phosphotransferase systems]]}},~~
−	~~{{SubtiWiki category\|[[utilization of specific carbon sources]]}},~~
−	~~{{SubtiWiki category\|[[transcription factors and their control]]}},~~
−	~~{{SubtiWiki category\|[[trigger enzyme]]}},~~
−	~~{{SubtiWiki category\|[[membrane proteins]]}},~~
−	~~{{SubtiWiki category\|[[phosphoproteins]]}}~~
−
−	~~= This gene is a member of the following [[regulons]] =~~
−	~~{{SubtiWiki regulon\|[[CcpA regulon]]}},~~
−	~~{{SubtiWiki regulon\|[[LicT regulon]]}}~~
−
−	~~=The gene=~~
−
−	~~=== Basic information ===~~
−
−	* '''Locus tag:''' BSU39270
−
−	~~===Phenotypes of a mutant ===~~
−
−	~~=== Database entries ===~~
−
−	* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]
−
−	* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]
−
−	~~=== Additional information===~~
−
−	~~=The protein=~~
−
−	~~=== Basic information/ Evolution ===~~
−
−	* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
−
−	* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
−
−	* '''Paralogous protein(s):'''
−
−	~~=== Extended information on the protein ===~~
−
−	* '''Kinetic information:'''
−
−	* '''Domains:'''
−
−	* '''Modification:'''
−
−	* '''Cofactor(s):'''
−
−	* '''Effectors of protein activity:'''
−
−	* '''[[SubtInteract\|Interactions]]:'''
−	** [[BglP]]-[[PtsH\|HPr]], [[BglP]]-[[LicT]]
−
−	* '''[[Localization]]:''' cell membrane {{PubMed\|23475962}}
−
−	~~=== Database entries ===~~
−
−	* '''Structure:'''
−
−	* '''UniProt:''' [http://www.uniprot.org/uniprot/P40739 P40739]
−
−	* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39270]
−
−	* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] 9
−
−	~~=== Additional information===~~
−
−	~~=Expression and regulation=~~
−
−	* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
−
−	* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 bglP] {{PubMed\|22383849}}
−
−	* '''[[Sigma factor]]:''' [[SigA]] {{PubMed\|7559347}}
−
−	* '''Regulation:'''
−	** repressed by glucose ([[CcpA]], [[LicT]]) {{PubMed\|7559347}}
−	** induced by salicin ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
−
−	* '''Regulatory mechanism:'''
−	** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region) {{PubMed\|7559347}}
−	** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH\| HPr]] {{PubMed\|8626332}}
−
−	* '''Additional information:'''
−	** An [[ncRNA\|antisense RNA]] is predicted for ''[[bglP]]'' {{PubMed\|20525796}}
−
−	~~=Biological materials =~~
−
−	* '''Mutant:''' GP475 (erm), available in the [[Stülke]] lab
−
−	* '''Expression vector:'''
−	** pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Stülke]] lab,
−	** pGP1300 (expression of ''[[bglP]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
−
−	* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab
−
−	* '''GFP fusion:'''
−
−	* '''CFP fusion:''' B. subtilis GP1266 bglP-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab
−
−	* '''YFP fusion:''' B. subtilis GP1274 bglP-yfp ermC- without terminator, available in [[Jörg Stülke]]'s lab
−
−	* '''two-hybrid system:'''
−
−	* '''Antibody:'''
−
−	~~=Labs working on this gene/protein=~~
−
−	~~=Your additional remarks=~~
−
−	~~=References=~~
−	~~<pubmed>16672620,20525796,10627040 7883710 8626332 7559347 22900538 23475962</pubmed>~~
−
−
−	~~[[Category:Protein-coding genes]]~~

@@ Line 18: / Line 18: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=BglP BglP]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bglP bglP]'''
 |-
 |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809

@@ Line 16: / Line 16: @@
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39270 bglP]
 |-
-|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/BglP BglP]
+|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=BglP BglP]
 |-
 |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''

@@ Line 33: / Line 33: @@
   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 |-
-|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglP_expression.png|500px]]
+|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:bglP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39270]]
 |-
 |}

@@ Line 26: / Line 26: @@
 |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
 |-
-|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39270 Advanced_DNA]
+|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39270 DNA_with_flanks]
 |-
 |-

@@ Line 154: / Line 154: @@
 =References=
-'''Additional publications:''' {{PubMed|22900538}}
+<pubmed>16672620,20525796,10627040 7883710 8626332 7559347 22900538 23475962</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 154: / Line 154: @@
 =References=
   <big>''Fabian M Rothe, Christoph Wrede, Martin Lehnik-Habrink, Boris Görke, Jörg Stülke''  </big>
   <big>'''Dynamic localization of a transcription factor in Bacillus subtilis:'''</big>
@@ Line 159: / Line 160: @@
   <big>J Bacteriol.: 2013, 195, Epub ahead of print. </big>
   [http://www.ncbi.nlm.nih.gov/pubmed/23475962 PubMed:23475962]
-<pubmed>16672620,20525796,10627040 7883710 8626332</pubmed>
+<pubmed>16672620,20525796,10627040 7883710 8626332 7559347</pubmed>
-<pubmed>7559347,</pubmed>
 [[Category:Protein-coding genes]]

@@ Line 160: / Line 160: @@
   [http://www.ncbi.nlm.nih.gov/pubmed/23475962 PubMed:23475962]
 <pubmed>16672620,20525796,10627040</pubmed>
-<pubmed>7883710 8626332 7559347, 22900538</pubmed>
+<pubmed>7559347, 22900538</pubmed>
 [[Category:Protein-coding genes]]