spoIIAA

168

anti-anti-SigF

Locus: BSU_23470

Molecular weight: 12.85 kDa

Isoelectric point: 5.86

Protein length: 117 aa

Gene length: 354 bp

Function: control of SigF activity

Product: anti-anti-SigF

Essential: no

Synonyms: spoIIAA

Genomic Context

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List of homologs in different organisms, belongs to COG5815 (Galperin et al., 2021)

Gene

Coordinates: 2,444,645  2,444,998

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed

The protein

Protein family

• anti-sigma-factor antagonist family (with RsbV, according to UniProt)

Domains

• STAS domain (aa 3-113) (according to UniProt)

Structure

• 1TIL (PDB) (complex with SpoIIAB, Geobacillus stearothermophilus) PubMed
• 1AUZ (PDB) (NMR)
• P10727 (AlphaFold)

Modification

• phosphorylation on (Ser-58 OR Ser-59) by SpoIIABPubMed, PubMed

Expression and Regulation

Operons

• Genes: spoIIAA-spoIIAB-sigF

  Description: PubMed

  Regulation

  • ''spoIIAA'': expressed early during sporulation

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: repression, in sinR regulon

  Sigma factors

  • SigF: sigma factor, in sigF regulon
  • SigG: sigma factor, in sigG regulon
  • SigH: sigma factor, in sigH regulon

• Genes: dacF-spoIIAA-spoIIAB-sigF

  Description: PubMed

  Regulation

  • ''spoIIAA'': expressed early during sporulation

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon
  • SigG: sigma factor, in sigG regulon

Biological materials

Mutant

• BKE23470 (spoIIAA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTCATTCCTCCTTGAT,  downstream forward: _UP4_CTCCTGACACTGGGGGTGGC
• BKK23470 (spoIIAA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTCATTCCTCCTTGAT,  downstream forward: _UP4_CTCCTGACACTGGGGGTGGC

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

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Original Publications

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