SubtiWiki - User contributions [en]

YqiD

2009-03-13T10:10:15Z

Mark Leaver:

* '''Description:''' Similar to an ''E. coli'' protein called IspA which is a farnasyl pyrophosphate synthase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yqiD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.021
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 816 bp, 272 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dxs]]'', ''[[yqiC]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqiD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqiD_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:yqiD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2525096-2525911

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11714]

=== Additional information===

Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In ''B. subtilis'' there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane.

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' None

* '''Cofactor(s):''' Magnesium

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''
** 1RQI: IspA from ''E. coli''. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed]

* '''Swiss prot entry:'''

* '''KEGG entry:''' K00795

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

All information about this gene is based on its sequence similarity to the IspA protein of ''E. coli''. There is no published data on the activity of the ''B. subtilis'' protein.

=References=

#Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV & Finn J (2004) Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis Journal of Biological Chemistry '''279(10)''': 8526-9 [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed]

MreD

2009-03-11T19:25:41Z

Mark Leaver:

* '''Description:''' MreD is a cell shape determining protein and is associated with the MreB cytoskeleton in B. subtilis and other rod shaped bacteria. 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rodB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 7.906
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 516 bp, 172 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[minC]]'', ''[[mreC]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:mreD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2858547-2859062

===Phenotypes of a mutant ===

the phenotype of ''mreD'' is similar to that of ''[[mreC]]''. ''mreD'' is essential under normal conditions [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]. Depletion of MreD leads to a progressive increase in the width and a decrease in the length of the cell and cells become lytic. In the depletion strain, lysis can be prevented and cell growth, but not cell shape, can be recovered by inculaion of Magnesium in the media. This shape defect is consistent with a role for ''mreD'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10328]

= Additional information=

===Function===

MreD functions in cell wall synthesis by, together with the MreB cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell. As a transmembrane protein MreD is thought to provide a patch on the membrane that MreB inteacts with. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.

===''mreD'' and the mapping of ''mre-min'' operon===

The ''mre-min'' operon was first sequenced by levin et al in 1992. The temperature sentitive ''rodB1'' mutation mapperd to that region.

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' None/ structural

* '''Protein family:''' COG2891

* '''Paralogous protein(s):''' None

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' None

* '''Swiss prot entry:''' Q01467

* '''KEGG entry:''' K03571

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[mreB]]''-''[[mreC]]''-'''mreD'''-''[[minC]]''-''[[minD]]''

* '''Sigma factor:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' A conditional mutant with an inframe deletion of ''mreD'' complemented by a xylose inducible copy at an ectopic locus (strain named 4352) is avaliable from the Errington Group.

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3416 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

User:Mark Leaver

2009-03-08T15:06:17Z

Mark Leaver:

==Mark Leaver==
*Institution: Newcastle University
*Position: Post Doc
*Degree: D.Phil Bacterial Genetics, Oxford University
*Undergraduate: Masters in Biochemistry, University of Bath
*Contact:-
**mark.leaver@ncl.ac.uk [mailto:mark.leaver@ncl.ac.uk send mail]
==Research Interests==
*Cell shape determination
*Cell wall synthesis
*Characteristics of the Last Uinversal Common Ancestor

YqiD

2009-03-08T14:56:27Z

Mark Leaver:

* '''Description:''' Similar to an ''E. coli'' protein called IspA which is a fanrasyl pyrophosphate synthase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yqiD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.021
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 816 bp, 272 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dxs]]'', ''[[yqiC]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqiD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqiD_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:yqiD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2525096-2525911

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqiD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11714]

=== Additional information===

Farnasyl pyrophosphate is 15 carbon compound (C15) that is an intermediate in the isoprenoid pathway. In ''B. subtilis'' there are two major isoprenoids, the synthesis of both require farnasyl pyrophosphate. The first is heptapreyl pyrophosphate (C35) which acts as a membrane anchor in menaquinone, an electron acceptor in the electron transport chain. The second is undecaprenyl pyrophosphate (C55) which is required to transport both the peptidoglycan precursor, Lipid II, and teichoic acid across the membrane.

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' dimethylallyl pyrophosphate + 2 isopentanyl pyrophosphate -> farnasyl pyrophosphate

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' None

* '''Cofactor(s):''' Magnesium

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''
** 1RQI: IspA from ''E. coli''. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed]

* '''Swiss prot entry:'''

* '''KEGG entry:''' K00795

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

All information about this gene is based on its sequence similarity to the IspA protein of ''E. coli''. There is no published data on the activity of the ''B. subtilis'' protein.

=References=

#Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV & Finn J (2004) Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis Journal of Biological Chemistry '''279(10)''': 8526-9 [http://www.ncbi.nlm.nih.gov/pubmed/14672944 PubMed]

MreC

2009-03-07T19:52:15Z

Mark Leaver:

* '''Description:''' MreC is a cell shape determining protein and is associated with the [[MreB]] cytoskeleton in ''B. subtilis'' and other rod shaped bacteria. 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 6.248
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mreD]]'', ''[[mreB]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreC_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2859062-2859931

===Phenotypes of a mutant ===

''mreC'' is essential under normal conditions [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]. Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[[rodA]]'' and the redundant gene pair ''[[pbpA]]'' and ''pbpH'' (also known as ''[[ykuA]]''). Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated [http://www.ncbi.nlm.nih.gov/pubmed/12867458 PubMed] and [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed]. ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Magnesium is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10327]

= Additional information=

===Function===

MreC functions in cell wall synthesis by, together with the MreB cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.

===MreC in other organisms===

MreC has been studied in other organisms where it has been shown to be important in cell shape determination.

*''Escherishia coli'' [http://www.ncbi.nlm.nih.gov/pubmed/15612918 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/17993535 PubMed]
*''Caulobacter cresentus'' [http://www.ncbi.nlm.nih.gov/pubmed/16344480 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/16344481 PubMed]
*''Rhodobacter spheroides'' [http://www.ncbi.nlm.nih.gov/pubmed/16484180 PubMed]
*''Streptomyces coelicolor'' [http://www.ncbi.nlm.nih.gov/pubmed/10954092 PubMed]

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' None/ structural protein

* '''Protein family:''' COG1793

* '''Paralogous protein(s):''' None

=== Extended information on the protein ===

* '''Kinetic information:''' None

* '''Domains:''' Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

* '''Modification:''' None

* '''Cofactor(s):''' None

* '''Effectors of protein activity:'''

* '''Interactions:''' Interacts with [[MreD]], and a subset of the PBPs [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]

* '''Localization:''' GFP-MreC localises to the cell membrane in a helical pattern [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''Structure:-'''
** 2J5U: MreC from ''Lysteria monocytogenes'' [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]
** 2QF4: MreC monomer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]
** 2QF5: MreC dimer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]

* '''Swiss prot entry:''' Q01466

* '''KEGG entry:''' K03570

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]''

* '''Sigma factor:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [[Errington]] lab [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''two-hybrid system:'''

* '''Antibody:''' antisera raised in rabit is avaliable from the [[Errington]] lab.

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

=Your additional remarks=

''mreC'' is an abbreviation of murein region e, gene C

=References=

# Lee JC & Stewart GC (2003) Essential nature of the ''mreC'' determinant of ''Bacillus subtilis'' ''Journal of Bacteriology'' '''185(15)''': 4490-8. [http://www.ncbi.nlm.nih.gov/pubmed/12867458 PubMed]
# Leaver M & Errington J (2005) Roles for MreC and MreD proteins in helical growth of the cylindrical cell wall in ''Bacillus subtilis'' ''Molecular Microbiology'' '''57(5)''': 1196-209 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed]
# Kruse T, Bork-Jensen J & Gerdes K (2005) The morphogenetic MreBCD proteins of ''Escherichia coli'' form an essential membrane-bound complex ''Molecular Microbiology'' '''55(1)''': 78-89 [http://www.ncbi.nlm.nih.gov/pubmed/15612918 PubMed]
#Bendezú FO & de Boer PA (2008) Conditional lethality, division defects, membrane involution, and endocytosis in ''mre'' and ''mrd'' shape mutants of ''Escherichia coli'' ''Journal of Bacteriology'' '''190(5)''': 1792-811 [http://www.ncbi.nlm.nih.gov/pubmed/17993535 PubMed]
#Divakaruni AV, Loo RR, Xie Y, Loo JA & Gober JW (2005) The cell-shape protein MreC interacts with extracytoplasmic proteins including cell wall assembly complexes in ''Caulobacter crescentus'' ''PNAS'' '''102(51)''': 18602-7 [http://www.ncbi.nlm.nih.gov/pubmed/16344480 PubMed]
#Dye NA, Pincus Z, Theriot JA, Shapiro L & Gitai Z (2005) Two independent spiral structures control cell shape in Caulobacter ''PNAS'' 102(51): 18608-13.
#Slovak PM, Porter SL & Armitage JP (2006) Differential localization of Mre proteins with PBP2 in ''Rhodobacter sphaeroides'' ''Journal of Bacteriology'' '''188(5)''': 1691-700
#Burger A, Sichler K, Kelemen G, Buttner M & Wohlleben W (2000) Identification and characterization of the ''mre'' gene region of ''Streptomyces coelicolor'' A3(2). Molecular and General Genetics '''263(6)''': 1053-60

MreC

2009-03-06T18:22:29Z

Mark Leaver:

* '''Description:''' MreC is a cell shape determining protein and is associated with the [[MreB]] cytoskeleton in ''B. subtilis'' and other rod shaped bacteria. 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 6.248
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mreD]]'', ''[[mreB]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreC_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2859062-2859931

===Phenotypes of a mutant ===

''mreC'' is essential under normal conditions [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]. Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and similar to other genes with roles in elongation like [[rodA]] and the redundant gene pair [[pbpA]] and pbpH (also known as [[ykuA]]). Electron microscopy of cells depleted of MreC show regions of the cell where a thick and irregular cell wall has accumulated [http://www.ncbi.nlm.nih.gov/pubmed/12867458 PubMed] and [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed]. ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Magnesium is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10327]

= Additional information=

===Function===

MreC functions in cell wall synthesis by acting, together with the MreB cytoskeleton, to localise the cell wall synthetic machinery to the correct part of the cell. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.

===MreC in other organisms===

MreC is also important for controling cell shape in other organisms.

*''Escherishia coli''
*''Caulobacter cresentus''
*''Rhodobacter spheroides''

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' None/ structural protein

* '''Protein family:'''

* '''Paralogous protein(s):''' None

=== Extended information on the protein ===

* '''Kinetic information:''' None

* '''Domains:''' Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' Interacts with [[MreD]], and a subset of the PBPs [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]

* '''Localization:''' GFP-MreC localises to the cell membrane in a helical pattern [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''Structure:-'''
** 2J5U: MreC from ''Lysteria monocytogenes'' [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]
** 2QF4: MreC monomer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]
** 2QF5: MreC dimer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]

* '''Swiss prot entry:''' Q01466

* '''KEGG entry:''' K03570

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]''

* '''Sigma factor:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [[Errington]] lab [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:''' A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''two-hybrid system:'''

* '''Antibody:''' antisera raised in rabit is avaliable from the [[Errington]] lab.

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

User:Mark Leaver

2009-03-06T14:10:58Z

Mark Leaver: Created page with '==Mark Leaver== *Institution: Newcastle University *Position: Post Doc *Degree: D.Phil Bacterial Genetics, Oxford University *Undergraduate: Masters in Biochemistry, University o...'

==Mark Leaver==
*Institution: Newcastle University
*Position: Post Doc
*Degree: D.Phil Bacterial Genetics, Oxford University
*Undergraduate: Masters in Biochemistry, University of Bath

==Research Interests==
*Cell shape determination
*Cell wall synthesis
*Characteristics of the Last Uinversal Common Ancestor

MreC

2009-03-06T12:50:33Z

Mark Leaver:

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 6.248
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mreD]]'', ''[[mreB]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreC_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreC_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:''' 2859062-2859931

===Phenotypes of a mutant ===

''mreC'' is essential under normal conditions [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]. Depletion of MreC leads to a progressive increse in the width and a decreas in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and similar to other genes with roles in elongation like [[rodA]] and the redundant gene pair [[pbpA]] and pbpH (also known as [[ykuA]]). Electron microscopy of cells depleted of MreC show regions of the cell where a thick and irregular cell wall has accumulated [http://www.ncbi.nlm.nih.gov/pubmed/12867458 PubMed] and [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed]. ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Magnesium is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10327]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' None

* '''Domains:''' Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' Interacts with MreD, and a subset of the PBPs [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]

* '''Localization:''' GFP-MreC localises to the cell membrane in a helical pattern [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''Structure:-'''
** 2J5U: MreC from ''Lysteria monocytogenes'' [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]
** 2QF4: MreC monomer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]
** 2QF5: MreC dimer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]

* '''Swiss prot entry:''' Q01466

* '''KEGG entry:''' K03570

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' mreB-'''mreC'''-mreD-minC-minD

* '''Sigma factor:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [[Errington]] lab [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

MreB

2009-03-06T09:08:44Z

Mark Leaver: /* Phenotypes of a mutant */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''divIVB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.901
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1011 bp, 337 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykpC]]'', ''[[abh]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreB_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed], the mutation can be suppressed by inactivation of ''[[ponA]]'', ''[[ptsI]]'', ''[[ccpA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] or addition of 5 mM Magnesium to the growth medium [http://www.ncbi.nlm.nih.gov/pubmed/15752190 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10326]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[Mbl]], [[MreBH]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[MreB]]-[[Mbl]], [[MreB]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]

* '''Localization:''' forms helical structures close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab

* '''Antibody:''' available in the [[Jeff Errington]] and [[Peter Graumann]] labs

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

=Your additional remarks=

=References=

# Carballido-Lopez, R., Formstone, A., Li, Y., Ehrlich, S. D., and Errington, J. (2006) Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE. Dev. Cell 11: 399-409. [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]
# Defeu Soufo, H. J., and P. Graumann. 2006. Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the funtion of MreB. Mol. Microbiol. 62: 1340-1356. [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
# Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in ''Bacillus subtilis'' by recruitment of PBP1 to the MreB helix. ''Mol. Microbiol.'' '''71:''' 1131-1144. [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

MreB

2009-03-06T09:07:23Z

Mark Leaver:

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''divIVB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.901
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1011 bp, 337 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykpC]]'', ''[[abh]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreB_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed], the mutation can be suppressed by inactivation of ''[[ponA]]'', ''[[ptsI]]'', ''[[ccpA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed] or 5 mM Magnesium [http://www.ncbi.nlm.nih.gov/pubmed/15752190 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10326]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[Mbl]], [[MreBH]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[MreB]]-[[Mbl]], [[MreB]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]

* '''Localization:''' forms helical structures close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab

* '''Antibody:''' available in the [[Jeff Errington]] and [[Peter Graumann]] labs

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

=Your additional remarks=

=References=

# Carballido-Lopez, R., Formstone, A., Li, Y., Ehrlich, S. D., and Errington, J. (2006) Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE. Dev. Cell 11: 399-409. [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]
# Defeu Soufo, H. J., and P. Graumann. 2006. Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the funtion of MreB. Mol. Microbiol. 62: 1340-1356. [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
# Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in ''Bacillus subtilis'' by recruitment of PBP1 to the MreB helix. ''Mol. Microbiol.'' '''71:''' 1131-1144. [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

MreB

2009-03-06T09:03:32Z

Mark Leaver:

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''divIVB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.901
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1011 bp, 337 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykpC]]'', ''[[abh]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mreB_protein.txt Protein sequence]'''
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
|-
|colspan="2" | '''Genetic context''' [[Image:mreB_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed], the mutation can be suppressed by inactivation of ''[[ponA]]'', ''[[ptsI]]'', ''[[ccpA]]'' or 5 mM Magnesium [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10326]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[Mbl]], [[MreBH]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[MreB]]-[[Mbl]], [[MreB]]-[[MreBH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]

* '''Localization:''' forms helical structures close to the inner surface of the cytoplasmic membrane [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jeff Errington]] lab

* '''Antibody:''' available in the [[Jeff Errington]] and [[Peter Graumann]] labs

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

=Your additional remarks=

=References=

# Carballido-Lopez, R., Formstone, A., Li, Y., Ehrlich, S. D., and Errington, J. (2006) Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE. Dev. Cell 11: 399-409. [http://www.ncbi.nlm.nih.gov/sites/entrez/16950129 PubMed]
# Defeu Soufo, H. J., and P. Graumann. 2006. Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the funtion of MreB. Mol. Microbiol. 62: 1340-1356. [http://www.ncbi.nlm.nih.gov/sites/entrez/17064365 PubMed]
# Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in ''Bacillus subtilis'' by recruitment of PBP1 to the MreB helix. ''Mol. Microbiol.'' '''71:''' 1131-1144. [http://www.ncbi.nlm.nih.gov/sites/entrez/19192185 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]