SubtiWiki - User contributions [en]

ComC

2010-07-14T11:33:50Z

Adr: /* Expression and regulation */

* '''Description:''' late competence gene required for processing and translocation of [[ComGC]], [[ComGD]], [[ComGE]] and [[ComGG]] [http://www.ncbi.nlm.nih.gov/pubmed/9723928 PubMed] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''comC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || processing protease
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || genetic transformation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 9.843
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 744 bp, 248 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIB]]'', ''[[folC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14767]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:comC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU28070

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/comC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10323]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine (according to Swiss-Prot)

* '''Protein family:''' peptidase A24 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P15378 P15378]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28070]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''comC'' {{PubMed|1694528}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1694528}}

* '''Regulation:'''

* '''Regulatory mechanism:'''
** ComK: transcription activation {{PubMed|8196543}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>9723928,16751195,7783624,2553669,1694528, 8196543 </pubmed>

[[Category:Protein-coding genes]]

YwiE

2010-03-07T14:22:09Z

Adr:

* '''Description:''' minor cardiolipin synthetase, general stress protein 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywiE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || minor cardiolipin synthetase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || phospholipiid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 58 kDa, 8.384
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1500 bp, 500 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjA]]'', ''[[narI]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15752]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywiE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU37240

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11305]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol (according to Swiss-Prot)

* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[ClsA]], [[YwjE]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P45860 P45860]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37240]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]

* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>14973018 , 15805528, 16514141 </pubmed>

[[Category:Protein-coding genes]]

YwpB

2010-03-07T14:22:04Z

Adr:

* '''Description:''' β-hydroxyacyl-(acyl carrier protein) dehydratase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywpB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || β-hydroxyacyl-ACP dehydratase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 9.36
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 396 bp, 132 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mscL]]'', ''[[rapD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15654]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ywpB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU36370

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12496]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' FabZ subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YcsD]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3D6X 3D6X] (from ''Campylobacter jejuni'', 45% identity, 67% similarity) {{PubMed|19280690}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P94584 P94584]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36370]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>17919287 19280690 </pubmed>
[[Category:Protein-coding genes]]

YwjE

2010-03-07T14:21:59Z

Adr:

* '''Description:''' minor cardiolipin synthetase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywjE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || minor cardiolipin synthetase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || phospholipid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.053
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1194 bp, 398 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fadF]]'', ''[[ywjD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15747]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywjE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU37190

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11310]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[ClsA]], [[YwiE]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P45865 P45865]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37190]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:''' [[SigG]] {{PubMed|15699190}}

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18820022,14973018 , 16514141 </pubmed>

[[Category:Protein-coding genes]]

YwaA

2010-03-07T14:21:54Z

Adr:

* '''Description:''' branched-chain amino acid aminotransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywaA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-0r ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid aminotransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of branched-chain amino acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 4.952
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1089 bp, 363 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dltE]]'', ''[[licH]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15881]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ywaA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38550

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywaA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10546]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

* '''Protein family:''' class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YbgE]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' S-cysteinylation after diamide stress (C104) [http://www.ncbi.nlm.nih.gov/sites/entrez/17611193 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3HT5 3HT5] (from '' Mycobacterium tuberculosis'', 42% identity, 58% similarity) {{PubMed|19923721}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39576 P39576]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38550]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.42 2.6.1.42]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''ywaA'' {{PubMed|9353933}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12670965,17611193,, </pubmed>

[[Category:Protein-coding genes]]

YqhY

2010-03-07T14:21:46Z

Adr:

* '''Description:''' unknown 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yqhY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.414
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 405 bp, 135 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nusB]]'', ''[[accC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14364]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:yqhY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24330

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11709]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' asp23 family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YloU]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54519 P54519]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24330]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>17114254, 7592499 </pubmed>

[[Category:Protein-coding genes]]

YhdN

2010-03-07T14:21:40Z

Adr:

* '''Description:''' general stress protein, aldo/keto reductase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yhdN''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || aldo/keto reductase, specific for NADP
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.77
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 993 bp, 331 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigM]]'', ''[[plsC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12792]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yhdN_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU09530

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yhdNO.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13020]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' aldo/keto reductase 2 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1PZ1 1PZ1]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P80874 P80874]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09530]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yhdN]]-[[plsC]]'' {{PubMed|10220166}}

* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|10220166,15805528}}

* '''Regulation:'''
** induced by stress ([[SigB]]) {{PubMed|10220166,15805528}}
** induced upon malonyl-CoA limitation ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>10220166,15019785,,12737802,15805528, </pubmed>

[[Category:Protein-coding genes]]

MutY

2010-03-07T14:21:34Z

Adr:

* '''Description:''' similar to A/G-specific adenine glycosylase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yfhQ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || error prevention oxidized guanine system
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.044
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1107 bp, 369 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfhP]]'', ''[[yfhS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12691]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yfhQ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU08630

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12891]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1RRS 1RRS] (complex with DNA, Geobacillus stearothermophilus)

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31584 O31584]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08630]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}

* '''[[Sigma factor]]:'''
**[[SigA]] {{PubMed|10463184}}
**[[SigG]] {{PubMed|1900507}}

* '''Regulation:'''
** [[YfhP]] may act as a negative transcription regulator of the ''[[yfhQ]]-[[fabL]]-[[sspE]]'' operon during exponential growth {{PubMed|10463184}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>10463184 19011023 </pubmed>
[[Category:Protein-coding genes]]

YbgE

2010-03-07T14:20:06Z

Adr:

* '''Description:''' branched-chain amino acid aminotransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ybgE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid aminotransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of branched-chain amino acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val], [http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.952
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1068 bp, 356 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybgB]]'', ''[[ybgF]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12033]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ybgE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU02390

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ybgE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12749]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

* '''Protein family:''' class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YwaA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31461 O31461]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02390]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.42 2.6.1.42]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''ybgE'' {{PubMed|15060025}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:''' pGP248 (in [[pAC5]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18083814,12618455,12670965,,15060025 ,12107147, </pubmed>

[[Category:Protein-coding genes]]

YcsD

2010-03-07T14:19:59Z

Adr:

* '''Description:''' β-hydroxyacyl-(acyl carrier protein) dehydratase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ycsD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || β-hydroxyacyl-ACP dehydratase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.687
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 390 bp, 130 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yczH]]'', ''[[ycsE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12211]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ycsD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU04030

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycsD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11225]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' FabZ subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YwpB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P42961 P42961]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04030]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''ycsD'' (according to [http://dbtbs.hgc.jp/COG/prom/ycsD.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>17919287</pubmed>
[[Category:Protein-coding genes]]

YbfM

2010-03-07T14:19:54Z

Adr:

* '''Description:''' similar to alkaline phosphatase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ybfM''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 9.482
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 486 bp, 162 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pssA]]'', ''[[psd]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12022]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ybfM_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU02280

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12741]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' dedA family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31453 O31453]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02280]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>14762009,, </pubmed>

[[Category:Protein-coding genes]]

UgtP

2010-03-07T14:19:50Z

Adr:

* '''Description:''' UDP-glucose diacylglycerol glucosyltransferase, growth-rate dpendent inhibitor of cell division 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ugtP''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypfP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || UDP-glucose diacylglycerol glucosyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of glycolipids and anchoring of lipoteichoic acid, inhibition of [[FtsZ]] assembly
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 8.398
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1146 bp, 382 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metA]]'', ''[[cspD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14110]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ugtP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU21920

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11611]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[EzrA]]-[[UgtP]], [[UgtP]]-[[UgtP]]

* '''Localization:''' cytoplasm (according to Swiss-Prot), membrane-bound protein, self-assembles into tightly wound spirals ''in vitro'' {{PubMed|17662947}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54166 P54166]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU21920]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

==Reviews==
<pubmed> 19680248 </pubmed>

==Original Publications==
<pubmed>9244290,18820022,17662947,9720862,, </pubmed>

[[Category:Protein-coding genes]]

TnrA

2010-03-07T14:19:40Z

Adr:

* '''Description:''' transcriptional pleiotropic regulator invoved in global nitrogen regulation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''tnrA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''scgR ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription activator/ repressor
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||regulation of nitrogen assimilation (positive regulation of nrgAB, nasBCDEF, gabP, ureABC, guaD; negative regulation of glnRA, gltAB)
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/cellwall.html Cell wall], [http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 10.235
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 330 bp, 110 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mgtE]]'', ''[[ykzB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13188]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:tnrA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU13310

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/tnrA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11805]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Genes/ operons controlled by TnrA ===

* '''Activation by TnrA:''' ''[[nrgA]]-[[nrgB]]''

* '''Repression by TnrA:''' ''[[degS]]-[[degU]]'', ''[[gltA]]-[[gltB]]''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' feedback-inhibited [[GlnA]] prevents TnrA from DNA binding

* '''Interactions:''' [[TnrA]]-[[GlnA]], [[TnrA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed], [[TnrA]]-[[GlnA]], this interaction results in loss of TnrA DNA-binding activity

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45666 Q45666]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13310]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''tnrA'' (according to [http://dbtbs.hgc.jp/COG/prom/tnrA.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** expression is autocativated (TnrA) and repressed by [[GlnR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16547045 PubMed]
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10671441,16547045 PubMed]

* '''Regulatory mechanism:'''
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/10671441,16547045 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP252 (in frame deletion), available in the [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP171 available in [[Stülke]] lab
** pGP229 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:''' available in the [[Karl Forchhammer]] lab

=Labs working on this gene/protein=

[[Susan Fisher]], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 10231480 18086213 </pubmed>
==Original publications==
<pubmed>12374841,15547269,9287005,12823818,12950915,10671441,16547045,11719184, 16547045 ,12139611, 8799114, 12823818,15150225, 11029411,17001076,15547269, 17085574
2573733, 8636055, 19233925, 16493705, 16885465, 6141156 18667567 </pubmed>
[[Category:Protein-coding genes]]

Spo0A

2010-03-07T14:19:23Z

Adr:

* '''Description:''' [[phosphorelay]] regulator, initiation of sporulation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''spo0A''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0C, spo0G, spoIIL, sof-1 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[phosphorelay]] response regulator
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism],[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress], [http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging],[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis] '''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.989
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 801 bp, 267 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiG]]'', ''[[spoIVB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14353]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:spo0A_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24220

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0A.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10765]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Genes controlled by Spo0A ===

* '''Activation by Spo0A:''' ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[spoIIE]]'', ''[[spoIIGA]]-[[sigE]]-[[sigG]]''

* '''Repression by Spo0A:''' ''[[abrB]], [[kinA]], [[kinC]], [[spo0A]]'', ''[[metS]]''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' receives phosphorylation from [[Spo0B]], dephosphorylation by [[Spo0E]], direct phosphorylation by [[KinC]] upon potassium leakage {{PubMed|19114652}}

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' the phosphorylation state affects DNA binding activity

* '''Interactions:''' [[KinC]]-[[Spo0A]] {{PubMed|19114652}}, [[Spo0A]]-[[Spo0E]], [[Spo0A]]-[[SigA]], [[Spo0A]]-[[Spo0B]]

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QMP 1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1FC3 1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1LQ1 1LQ1] (complex with DNA)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P06534 P06534]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24220]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''spo0A'' {{PubMed|11254139}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11254139,1569009}}, [[SigH]] {{PubMed|11254139,1569009}}

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

* [[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
* [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
* [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed>19995980 20030732 20157337 </pubmed>

==Original Publications==
<pubmed>19528067, 19581368 , 8730857,2106683,3157992, 11254139,1569009, 12067336,10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 14651647,1537790,9658000, 8509330,14762002, 1846779, 2981817, 15687200, 9477965,8600030, 18840696, 1391039, 14679239, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 </pubmed>

[[Category:Protein-coding genes]]

SspE

2010-03-07T14:18:29Z

Adr:

* '''Description:''' small acid-soluble spore protein (major gamma-type SASP) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sspE''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (major gamma-type SASP)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 8.945
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 252 bp, 84 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabL]]'', ''[[ygaB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12694]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sspE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU08660

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10789]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' gamma-type SASP family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P07784 P07784]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08660]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon'''
** ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}
** ''[[fabL]]-[[sspE]]'' {{PubMed|10463184}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10463184}}, [[SigG]] {{PubMed|15699190}}

* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>3106326,1900507,2456528,2497051,11092849,,12107147,10463184 </pubmed>

[[Category:Protein-coding genes]]

Spo0A

2010-03-07T14:18:22Z

Adr:

* '''Description:''' [[phosphorelay]] regulator, initiation of sporulation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''spo0A''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0C, spo0G, spoIIL, sof-1 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[phosphorelay]] response regulator
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism],[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress], [http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging],[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis] '''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.989
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 801 bp, 267 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiG]]'', ''[[spoIVB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14353]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:spo0A_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24220

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0A.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10765]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Genes controlled by Spo0A ===

* '''Activation by Spo0A:''' ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[spoIIE]]'', ''[[spoIIGA]]-[[sigE]]-[[sigG]]''

* '''Repression by Spo0A:''' ''[[abrB]], [[kinA]], [[kinC]], [[spo0A]]'', ''[[metS]]''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' receives phosphorylation from [[Spo0B]], dephosphorylation by [[Spo0E]], direct phosphorylation by [[KinC]] upon potassium leakage {{PubMed|19114652}}

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' the phosphorylation state affects DNA binding activity

* '''Interactions:''' [[KinC]]-[[Spo0A]] {{PubMed|19114652}}, [[Spo0A]]-[[Spo0E]], [[Spo0A]]-[[SigA]], [[Spo0A]]-[[Spo0B]]

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QMP 1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1FC3 1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [http://www.rcsb.org/pdb/explore.do?structureId=1LQ1 1LQ1] (complex with DNA)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P06534 P06534]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24220]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''spo0A'' {{PubMed|11254139}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11254139,1569009}}, [[SigH]] {{PubMed|11254139,1569009}}

* '''Regulation:'''
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1391039 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

* [[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
* [[Imrich Barak]], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
* [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed>19995980 20030732 20157337 </pubmed>

==Original Publications==
<pubmed>19528067, 19581368 , 8730857,2106683,3157992, 11254139,1569009, 12067336,10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 14651647,1537790,9658000, 8509330,14762002, 1846779, 2981817, 15687200, 9477965,8600030, 18840696, 1391039, 14679239, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 </pubmed>

[[Category:Protein-coding genes]]

PssA

2010-03-07T14:16:59Z

Adr:

* '''Description:''' phosphatidylserine synthase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pssA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pss ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylserine synthase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 7.881
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 531 bp, 177 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfK]]'', ''[[ybfM]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12021]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:pssA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU02270

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11012]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine (according to Swiss-Prot)

* '''Protein family:''' CDP-alcohol phosphatidyltransferase class-I family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39823 P39823]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02270]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.8 2.7.8.8]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed>9370336 , </pubmed>
==Original publications==
<pubmed>14762009,18820022,9422599 ,8829529 </pubmed>

[[Category:Protein-coding genes]]

Ptb

2010-03-07T14:16:49Z

Adr:

* '''Description:''' phosphate butyryltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptb''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiS, bkd ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphate butyryltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 7.936
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 897 bp, 299 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bcd]]'', ''[[bkdR]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14340]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ptb_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24090

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11722]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Butanoyl-CoA + phosphate = CoA + butanoylphosphate (according to Swiss-Prot)

* '''Protein family:''' phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1YCO 1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54530 P54530]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24090]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.19 2.3.1.19]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}

* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]

* '''Regulation:'''
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
** [[BkdR]]: transcription activation {{PubMed|10094682}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12427936,15241682,12823818,10094682, </pubmed>

[[Category:Protein-coding genes]]

Psd

2010-03-07T14:16:43Z

Adr:

* '''Description:''' phosphatidylserine decarboxylase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''psd''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylserine decarboxylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 7.27
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 789 bp, 263 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybfM]]'', ''[[ybfN]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12023]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:psd_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU02290

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pssA-ybfM-psd.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11013]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Phosphatidyl-L-serine = phosphatidylethanolamine + CO2 (according to Swiss-Prot)

* '''Protein family:''' Type 1 subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39822 P39822]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU02290]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.65 4.1.1.65]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[pssA]]-[[ybfM]]-[[psd]]'' {{PubMed|14762009}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|14762009}}, [[SigX]] {{PubMed|14762009}}

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>14762009,18820022,9422599 , </pubmed>

[[Category:Protein-coding genes]]

PlsY

2010-03-07T14:16:37Z

Adr:

* '''Description:''' acylphosphate:glycerol-phosphate acyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''plsY ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yneS''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acylphosphate:glycerol-phosphate acyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 10.408
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yneR]]'', ''[[yneT]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13690]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yneS_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU18070

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11826]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acyl-phosphate + sn-glycerol 3-phosphate = acyl-sn-glycerol 3-phosphate + phosphate (according to UniProt)

* '''Protein family:''' UPF0078 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[PlsY]]-[[PlsX]] {{PubMed|19282621}}

* '''Localization:''' cell membrane {{PubMed|19820159}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/Q45064 Q45064]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18070]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in Matsumoto lab {{PubMed|19282621}}

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed> 19282621, 17005971, 17557823, 19820159, </pubmed>

[[Category:Protein-coding genes]]

PlsC

2010-03-07T14:14:35Z

Adr:

* '''Description:''' acyl-ACP:1-acylglycerolphosphate acyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''plsC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhdO''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-ACP:1-acylglycerolphosphate acyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 9.976
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 597 bp, 199 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdN]]'', ''[[yhdP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12793]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yhdO_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU09540

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yhdNO.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13021]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07584 O07584]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09540]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.51 2.3.1.51]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yhdN]]-[[plsC]]'' {{PubMed|10220166}}

* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|10220166,15805528}}

* '''Regulation:'''
** induced by stress ([[SigB]]) {{PubMed|10220166,15805528}}
** induced upon malonyl-CoA limitation ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12737802,17557823 </pubmed>

[[Category:Protein-coding genes]]

PlsX

2010-03-07T14:14:30Z

Adr:

* '''Description:''' acyl-acyl carrier protein (ACP):phosphate acyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''plsX''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-acyl carrier protein (ACP):phosphate acyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.492
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fapR]]'', ''[[fabD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13462]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:plsX_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15890

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11843]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' plsX family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[PlsX]]-[[PlsY]] {{PubMed|19282621}}

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1VI1 1VI1]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71018 P71018]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15890]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}

* '''Regulation:'''
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}

* '''Regulatory mechanism:'''
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in Matsumoto lab {{PubMed|19282621}}

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12737802, 19282621, 17645809, 17557823, 8759840 16932747 19850612 16091051</pubmed>

[[Category:Protein-coding genes]]

PgsA

2010-03-07T14:14:22Z

Adr:

* '''Description:''' phosphatidylglycerophosphate synthase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pgsA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ymfN ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidylglycerophosphate synthase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.163
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rodZ]]'', ''[[cinA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13565]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:pgsA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU16920

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11373]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate (according to Swiss-Prot)

* '''Protein family:''' CDP-alcohol phosphatidyltransferase class-I family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane {{PubMed|19820159}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P46322 P46322]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16920]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.5 2.7.8.5]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>19745567 19820159 </pubmed>
[[Category:Protein-coding genes]]

PgcA

2010-03-07T14:14:15Z

Adr:

* '''Description:''' alpha-phosphoglucomutase, required for UDP-glucose synthesis 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pgcA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhxB''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || alpha-phosphoglucomutase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 4.913
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1695 bp, 565 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glpD]]'', ''[[yhcY]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12759]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:yhxB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU09310

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10189]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate (according to Swiss-Prot)

* '''Protein family:''' phosphohexose mutase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P18159 P18159]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09310]

* '''E.C. number:''' [http://www.expasy.org/enzyme/5.4.2.2 5.4.2.2]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>,15175311,17726680 16493705, 15640167 17662947 </pubmed>

[[Category:Protein-coding genes]]

MprF

2010-03-07T14:14:09Z

Adr:

* '''Description:''' synthesis of lysylphosphatidylglycerol (L-PG) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mprF''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfiW, yfiX''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || lysyl-phosphatidylglycerol synthase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || lysyl-phosphatidylglycerol lipid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' ||
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfiV]]'', ''[[yfiY]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAX52582]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yfiW_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU08425

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfiWX.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12899 old_YfiW][http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12900 old_YfiX]

=== Additional information===

Gene was re-assembled due to sequencing error in original sequence (it encompasses the genes annotated as ''yfiX'' and ''yfiW'' in SubtiList) [http://www.ncbi.nlm.nih.gov/sites/entrez/12427923 PubMed]

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/C0H3X7 C0H3X7]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08425]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''mprF'' (according to [http://dbtbs.hgc.jp/COG/prom/yfiWX.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>19164152,18820022, 19734140, 18305156 12427923 </pubmed>

[[Category:Protein-coding genes]]

LtaS

2010-03-07T14:14:04Z

Adr:

* '''Description:''' lipoteichoic acid synthase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ltaS''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yflE''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || major lipoteichoic acid synthase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lipoteichoic acid
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 5.202
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1947 bp, 649 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nagP]]'', ''[[yflD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12600]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yflE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yflE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12940]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' LTA synthase family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YfnI]], [[YqgS]], [[YvgJ]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' phosphorylated on Thr-297 {{PubMed|19229300}}

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot), extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=69910&ordinalpos=1&log$=structuread_title 2W8D]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34952 O34952]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07710]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''ltaS'' (according to [http://dbtbs.hgc.jp/COG/prom/yflE.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>19229300,17483484 , 19168632 </pubmed>

[[Category:Protein-coding genes]]

GpsA

2010-03-07T14:12:29Z

Adr:

* '''Description:''' glycerol-3-phosphate dehydrogenase (NAD) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''gpsA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glycerol-3-phosphate dehydrogenase (NAD)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 6.231
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1035 bp, 345 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yphE]]'', ''[[yphC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14199]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:gpsA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU22830

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11366]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)

* '''Protein family:''' NAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3K96 3K96] (from ''Coxiella burnetii RSA 493'', 37% identity, 59% similarity)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P46919 P46919]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22830]

* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.94 1.1.1.94]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed> 7592341 </pubmed>
[[Category:Protein-coding genes]]

FapR

2010-03-07T14:12:24Z

Adr:

* '''Description:''' repressor of fatty acid synthetic genes 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fapR ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpC''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.393
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 564 bp, 188 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recG]]'', ''[[plsX]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13461]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ylpC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15880

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13400]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' FapR regulates the expression of at least 10 genes (the ''fap'' regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the ''fap'' regulon.

* '''Protein family:''' fapR family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' malonyl-CoA and malonyl-[[AcpA|ACP]] act as the molecular inducer of the FapR regulon {{PubMed|20201588}}

* '''Interactions:''' malonyl-[[AcpA|ACP]]-[[FapR]] {{PubMed|20201588}}

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2F3X 2F3X] (complex with effector), [http://www.rcsb.org/pdb/explore.do?structureId=2F41 2F41]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34835 O34835]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15880]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}

* '''Regulation:'''
** repressed in the absence of malonyl-CoA or malonyl-[[AcpA|ACP]] ([[FapR]]) {{PubMed|20201588}}
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}

* '''Regulatory mechanism:'''
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12737802, 18941141, 18820022, 18384517, 17919287, 16932747,12737802 19850612 16091051 20201588</pubmed>

[[Category:Protein-coding genes]]

FabL

2010-03-07T14:12:15Z

Adr:

* '''Description:''' enoyl-acyl carrier protein reductase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabL''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yfhR, ygaA ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enoyl-acyl carrier protein reductase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.967
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 750 bp, 250 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfhS]]'', ''[[sspE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12693]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabL_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU08650

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfhQ-fabL-sspE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12221]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):''' [[FabI]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71079 P71079]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU08650]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''
** ''[[yfhQ]]-[[fabL]]-[[sspE]]'' {{PubMed|10463184}}
** ''[[fabL]]-[[sspE]]'' {{PubMed|10463184}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10463184}}, [[SigG]] {{PubMed|15699190}}

* '''Regulation:'''
**repressed by [[YfhP]] during exponential growth {{PubMed|10463184}}
**repressed by [[SpoVT]] during forespore expression {{PubMed|8755877}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>17114254, 10463184 15699190 8755877 </pubmed>

[[Category:Protein-coding genes]]

FabI

2010-03-07T14:12:04Z

Adr:

* '''Description:''' enoyl-acyl carrier protein reductase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabI''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjbW ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enoyl-acyl carrier protein reductase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.605
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 774 bp, 258 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiD]]'', ''[[cotO]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13029]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabI_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11720

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabI.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13152]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)

* '''Protein family:''' FabI subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[FabL]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''
** inhibited by triclosan {{PubMed|11325930}}

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1DFI 1DFI] (the enzyme from ''E. coli'' in complex with NAD) {{PubMed|8953047}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54616 P54616]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11720]

* '''E.C. number:''' [http://www.expasy.org/enzyme/1.3.1.9 1.3.1.9]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fabI]]'' {{PubMed|12737802}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>12737802,17114254, 8953047 </pubmed>

[[Category:Protein-coding genes]]

FabHB

2010-03-07T14:11:55Z

Adr:

* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabHB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhfB '', ''fabH2 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase III
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.684
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 975 bp, 325 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhgE]]'', ''[[yhfC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12857]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabHB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU10170

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13048]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2 (according to Swiss-Prot)

* '''Protein family:''' fabH family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[FabHA]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07600 O07600]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10170]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.180 2.3.1.180], [http://www.expasy.org/enzyme/2.3.1.41 2.3.1.41]

=== Additional information===
* affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis {{PubMed|19820084}}

=Expression and regulation=

* '''Operon:''' ''fabHB'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed unless the cells experience a lack of malonyl-CoA ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>12737802,17114254,10629181, 10673437,19820084 </pubmed>

[[Category:Protein-coding genes]]

FabHA

2010-03-07T14:11:43Z

Adr:

* '''Description:''' beta-ketoacyl-acyl carrier protein synthase III 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabHA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjaX '', ''fabH1 ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase III
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.045
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 936 bp, 312 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjzB]]'', ''[[fabF]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12974]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:fabHA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11330

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13127]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2 (according to Swiss-Prot)

* '''Protein family:''' fabH family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[FabHB]], one of the two proteins has to be present for viability [http://www.ncbi.nlm.nih.gov/sites/entrez/17114254 PubMed]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1EBL 1EBL] (FabH from ''E. coli'') {{PubMed|10673437}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34746 O34746]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11330]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.180 2.3.1.180], [http://www.expasy.org/enzyme/2.3.1.41 2.3.1.41]

=== Additional information===
* affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis {{PubMed|19820084}}

=Expression and regulation=

* '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed|12737802}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12737802}}

* '''Regulation:'''
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
** inhibited by cerulenin {{PubMed|11325930}}

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>12737802,17114254,10629181, 10673437,19820084 </pubmed>

[[Category:Protein-coding genes]]

FabG

2010-03-07T14:10:34Z

Adr:

* '''Description:''' beta-ketoacyl-acyl carrier protein reductase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabG''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpF ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein reductase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 8.091
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 738 bp, 246 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabD]]'', ''[[acpA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13464]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabG_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15910

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11535]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH (according to Swiss-Prot)

* '''Protein family:''' short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1I01 1I01] (the enzyme from ''E. coli'') {{PubMed|11669613}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P51831 P51831]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15910]

* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.100 1.1.1.100]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}

* '''Regulation:'''
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}

* '''Regulatory mechanism:'''
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>12737802, 16932747 19850612 11669613 16091051</pubmed>

[[Category:Protein-coding genes]]

FabF

2010-03-07T14:10:14Z

Adr:

* '''Description:''' beta-ketoacyl-acyl carrier protein synthase II 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabF''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjaY ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase II
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.768
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1239 bp, 413 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabHA]]'', ''[[yjaZ]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12975]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabF_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11340

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13128]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein] (according to Swiss-Prot)

* '''Protein family:''' beta-ketoacyl-ACP synthases family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OX0 1OX0] (the protein from ''Streptococcus pneumoniae'') {{PubMed|12837788}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34340 O34340]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11340]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.179 2.3.1.179]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed|12737802}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12737802}}

* '''Regulation:'''
** expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 17919287</pubmed>
==Original Publications==
<pubmed>18384517, 12837788,12737802, </pubmed>

[[Category:Protein-coding genes]]

CodY

2010-03-07T14:10:01Z

Adr:

* '''Description:''' regulation of a large regulon in response to branched-chain amino acid limitation 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''codY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional pleiotropic repressor
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of a large regulon in response to
branched-chain amino acid limitation

to the presence of branched-chain amino acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 4.75
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[clpY]]'', ''[[flgB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13490]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:codY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU16170

===Phenotypes of a mutant ===

No swarming motility on B medium. [http://www.ncbi.nlm.nih.gov/sites/entrez/19202088 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/codV-clpQY-codY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10968]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' codY family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Genes/ operons controlled by CodY ===

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' contains a GAF domain (ligand binding domain)

* '''Modification:''' phosphorylation on Ser-215 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' GTP and branched chained amino acids (BCAA) increase the affinity of CodY for its DNA target sequences {{PubMed|11331605,15228537}}

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2B0L 2B0L] (C-terminal DNA-binding domain), [http://www.rcsb.org/pdb/explore.do?structureId=2GX5 2GX5] (N-terminal Gaf domain)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39779 P39779]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16170]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[codV]]-[[clpQ]]-[[clpY]]-[[codY]]'' {{PubMed|7783641}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7783641}}

* '''Regulation:'''
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11331605 PubMed]

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/11331605 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP566, available in [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP245, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[[Tony Wilkinson]], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]

[[Oscar Kuipers]], University of Groningen, The Netherlands
[http://molgen.biol.rug.nl/molgen/index.php Homepage]

=Your additional remarks=

=References=

<pubmed>19542274,17493123,18641142,19202088,16995897,17982469,18083814,17993518,9287005,12618455,11331605,17218307,19500589,12591885,19202088,12618455,15802253,11331605,15228537,8793880,15937175,15916605,15916606,11331605,15228537 19749041 7783641</pubmed>

[[Category:Protein-coding genes]]

FabD

2010-03-07T14:09:53Z

Adr:

* '''Description:''' malonyl CoA-acyl carrier protein transacylase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fabD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpE ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || malonyl CoA-acyl carrier protein transacylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.515
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 951 bp, 317 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[plsX]]'', ''[[fabG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13463]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:fabD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15900

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11836]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein] (according to Swiss-Prot)

* '''Protein family:''' fabD family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1MLA 1MLA] (the enzyme from ''E. coli'') {{PubMed|7768883}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71019 P71019]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.39 2.3.1.39]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|12737802,19850612}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16932747}}

* '''Regulation:'''
** induced if the cells experience an accumulation of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
** expressed at high cell density ([[ComA]]) {{PubMed|16091051}}

* '''Regulatory mechanism:'''
** [[ComA]]: transcription activation ([[ComA]]) {{PubMed|16091051}}
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed>12737802, 16932747 19850612 7768883 16091051</pubmed>

[[Category:Protein-coding genes]]

ClsA

2010-03-07T14:09:25Z

Adr:

* '''Description:''' cardiolipin synthase, major enzyme 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''clsA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywnE ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cardiolipin synthase, major enzyme
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 9.554
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1446 bp, 482 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywnF]]'', ''[[mta]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15676]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywnE_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU36590

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12483]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol (according to Swiss-Prot)

* '''Protein family:''' Cardiolipin synthase subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YwiE]], [[YwjE]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71040 P71040]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36590]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>18820022,14973018 , </pubmed>

[[Category:Protein-coding genes]]

CdsA

2010-03-07T14:09:19Z

Adr:

* '''Description:''' phosphatidate cytidylyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''cdsA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphatidate cytidylyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 7.866
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 807 bp, 269 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[uppS]]'', ''[[ispC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13527]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:cdsA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU16540

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12575]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' CTP + phosphatidate = diphosphate + CDP-diacylglycerol (according to Swiss-Prot)

* '''Protein family:''' CDS family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane {{PubMed|19820159}}

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31752 O31752]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16540]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.41 2.7.7.41]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:''' the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12884008,,19820159 </pubmed>

[[Category:Protein-coding genes]]

BkdR

2010-03-07T14:08:16Z

Adr:

* '''Description:''' [[transcription factors activating transcription at SigL-dependent promoters |transcriptional activator]] of the ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' operon 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bkdR''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiR ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[transcription factors activating transcription at SigL-dependent promoters |transcriptional activator]] (for [[SigL]]-dependent promoter))
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of branched-chain amino acid utilization,
required for survival at low temperatures)
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 77 kDa, 6.237
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2076 bp, 692 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptb]]'', ''[[yqzF]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14341]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:bkdR_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24100

===Phenotypes of a mutant ===

The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bkdR.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11721]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[BkdR]]-[[SigL]] (activation)

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54529 P54529]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24100]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''bkdR'' {{PubMed|12823818}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16585774,10094682,,12823818 , </pubmed>

[[Category:Protein-coding genes]]

Buk

2010-03-07T14:07:10Z

Adr:

* '''Description:''' butyrate kinase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''buk''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiU, bkd ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || butyrate kinase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.466
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1089 bp, 363 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lpdV]]'', ''[[bcd]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14338]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:buk_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24070

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11724]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP + butanoate = ADP + butanoyl phosphate (according to Swiss-Prot)

* '''Protein family:''' acetokinase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1X9J 1X9J] (from ''Thermotoga maritima'', 52% identity, 69% similarity)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54532 P54532]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24070]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.7 2.7.2.7]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}

* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]

* '''Regulation:'''
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
** [[BkdR]]: transcription activation {{PubMed|10094682}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12427936,15241682,10094682,12823818, </pubmed>

[[Category:Protein-coding genes]]

BrnQ

2010-03-07T14:06:42Z

Adr:

* '''Description:''' branched-chain amino acid [[transport proteins|transporter]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''brnQ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrdJ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid [[transport proteins|transporter]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of branched-chain amino acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.777
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1320 bp, 440 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrdK]]'', ''[[azlD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14610]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:brnQ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU26690

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11918]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[BraB]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P94499 P94499]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26690]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[azlB]]-[[azlC]]-[[azlD]]-[[brnQ]]-[[yrdK]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:''' repressed by [[AzlB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''Regulatory mechanism:''' [[AzlB]]: transcriptional repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed> 9287000 </pubmed>
[[Category:Protein-coding genes]]

BraB

2010-03-07T14:06:36Z

Adr:

* '''Description:''' branched-chain amino acid [[transport proteins|transporter]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''braB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid [[transport proteins|transporter]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of branched-chain amino acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 9.65
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1335 bp, 445 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nifZ]]'', ''[[ezrA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14938]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:braB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29600

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/braB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12573]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' branched chain amino acid transporter family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[BrnQ]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34545 O34545]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29600]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[braB]]'' {{PubMed|9387221}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed> 9387221 </pubmed>
[[Category:Protein-coding genes]]

Bcd

2010-03-07T14:05:57Z

Adr:

* '''Description:''' valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bcd''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''bkd, yqiT ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 4.942
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1092 bp, 364 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[buk]]'', ''[[ptb]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14339]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:bcd_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24080

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11723]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)

* '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1LEH 1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) {{PubMed|8591046}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P54531 P54531]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24080]

* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.9 1.4.1.9]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}

* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]

* '''Regulation:'''
** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
** [[BkdR]]: transcription activation {{PubMed|10094682}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12427936,15241682,10094682,12823818, </pubmed>

[[Category:Protein-coding genes]]

AzlD

2010-03-07T14:05:51Z

Adr:

* '''Description:''' branched-chain amino acid transport 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''azlD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrdI ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || branched-chain amino acid transport
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.962
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 330 bp, 110 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[brnQ]]'', ''[[azlC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14611]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:azlD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU26700

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11916]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' azlD/HI1737/HP1330 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07923 O07923]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU26700]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[azlB]]-[[azlC]]-[[azlD]]-[[brnQ]]-[[yrdK]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:''' repressed by [[AzlB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''Regulatory mechanism:''' [[AzlB]]: transcriptional repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9287000 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>9287000,, </pubmed>

[[Category:Protein-coding genes]]

AcpS

2010-03-07T14:05:44Z

Adr:

* '''Description:''' acyl-carrier protein synthase, 4'-phosphopantetheine transferases 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acpS''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydcB ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl-carrier protein synthase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 9.915
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 363 bp, 121 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ydcA]]'', ''[[ydcC]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12269]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acpS_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU04620

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12089]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein] (according to Swiss-Prot)

* '''Protein family:''' AcpS family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):''' magnesium {{PubMed|11489886}}

* '''Effectors of protein activity:'''

* '''Interactions:''' [[AcpA]]-[[AcpS]] {{PubMed|10997907}}

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1F7T 1F7T] {{PubMed|10997907}}, [http://www.rcsb.org/pdb/explore.do?structureId=1F80 1F80] (complex with [[AcpA]]) {{PubMed|10997907}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P96618 P96618]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04620]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.8.7 2.7.8.7]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed>11489886,15955059,10997907,11867633,, </pubmed>

[[Category:Protein-coding genes]]

AcpA

2010-03-07T14:05:33Z

Adr:

* '''Description:''' acyl carrier protein 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acpA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''acpP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acyl carrier protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 3.594
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 231 bp, 77 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabG]]'', ''[[rnc]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13465]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acpA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15920

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11536]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[AcpA]]-[[AcpS]] {{PubMed|10997907}}, [[AcpA]]-[[BioI]] {{PubMed|18838690}}, malonyl-[[AcpA|ACP]]-[[FapR]] {{PubMed|20201588}}

* '''Localization:''' cytoplasm (according to Swiss-Prot), during sporulation in the mother cell {{PubMed|19850612}}

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1F80 1F80] (complex with [[AcpS]]) {{PubMed|10997907}}, [http://www.rcsb.org/pdb/explore.do?structureId=3EJE 3EJE] (complex with [[BioI]]) {{PubMed|18838690}}, [http://www.rcsb.org/pdb/explore.do?structureId=1HY8 1HY8]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P80643 P80643]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15920]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''
** ''[[fapR]]-[[plsX]]-[[fabD]]-[[fabG]]-[[acpA]]'' {{PubMed|19850612}}
** ''[[acpA]]'' {{PubMed|19850612}}

* '''[[Sigma factor]]:'''
** ''[[fapR]]'': [[SigA]] {{PubMed|16932747}}

* '''Regulation:'''
** ''[[fapR]]'': repressed in the absence of malonyl-CoA or malonyl-[[AcpA|ACP]] ([[FapR]]) {{PubMed|12737802}}

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression {{PubMed|12737802}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
* [[Diego de Mendoza]], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed>10997907,19850612, 12737802 18838690 20201588 </pubmed>

[[Category:Protein-coding genes]]

AccA

2010-03-07T14:03:41Z

Adr:

* '''Description:''' acetyl-CoA carboxylase (alpha subunit) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''accA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (alpha subunit))
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.087
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 975 bp, 325 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pfkA]]'', ''[[accD]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14880]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:accA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29200

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accDA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12557]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA (according to Swiss-Prot)

* '''Protein family:''' accA family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)

* '''Localization:''' cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OD2 1OD2] (carboxytransferase from yeast, equivalent to [[AccA]]-[[AccD]]) {{PubMed|12663926}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34847 O34847]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29200]

* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[accD]]-[[accA]]'' (according to [http://dbtbs.hgc.jp/COG/prom/accDA.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903, 17919287 </pubmed>

==Original Publications==
<pubmed>15066985,,14651647,16479537 12663926 </pubmed>

[[Category:Protein-coding genes]]

AccB

2010-03-07T14:03:29Z

Adr:

* '''Description:''' acetyl-CoA carboxylase (biotin carboxyl carrier subunit) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''accB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fabE, yqhW ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.394
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 477 bp, 159 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accC]]'', ''[[spoIIIAH]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14366]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:accB_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24350

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11383]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BDO 1BDO] (the protein from ''E. coli'') {{PubMed|8747466}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P49786 P49786]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24350]

* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]

=== Additional information===

AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [[SPINE]].

=Expression and regulation=

* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed>7592499, 8747466, </pubmed>

[[Category:Protein-coding genes]]

AccC

2010-03-07T14:03:17Z

Adr:

* '''Description:''' acetyl-CoA carboxylase (biotin carboxylase subunit) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''accC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqhX ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (biotin carboxylase subunit)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 4.72
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1350 bp, 450 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqhY]]'', ''[[accB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14365]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:accC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU24340

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accBC-yqhY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11384]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

* '''Protein family:'''

* '''Paralogous protein(s):''' [[YotE]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1BNC 1BNC] (the protein from ''E. coli'') {{PubMed|7915138}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P49787 P49787]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24340]

* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.4.14 6.3.4.14], [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[accB]]-[[accC]]-[[yqhY]]'' {{PubMed|7592499}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed> 7592499, 7915138, </pubmed>

[[Category:Protein-coding genes]]

AccD

2010-03-07T14:03:06Z

Adr:

* '''Description:''' acetyl-CoA carboxylase (beta subunit) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''accD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yttI ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA carboxylase (beta subunit))
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || production of malonyl-CoA, the substrate for fatty acid biosynthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.344
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 786 bp, 262 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accA]]'', ''[[ytsJ]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14881]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:accD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29210

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/accDA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13926]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' ([[AccA]]-[[AccD]])-[[AccB]]-[[AccC]] (acetyl-CoA carboxylase complex)

* '''Localization:''' Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] Membrane-proximal (Spotty) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OD2 1OD2] (carboxytransferase from yeast, equivalent to [[AccA]]-[[AccD]]) {{PubMed|12663926}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SP93 C0SP93]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29210]

* '''E.C. number:''' [http://www.expasy.org/enzyme/6.4.1.2 6.4.1.2]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[accD]]-[[accA]]'' (according to [http://dbtbs.hgc.jp/COG/prom/accDA.html DBTBS])

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Regulatory mechanism:'''
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed> 15952903 </pubmed>
==Original Publications==
<pubmed>15066985, 12663926,14651647,16479537</pubmed>

[[Category:Protein-coding genes]]