SubtiWiki - User contributions [en]

Zwf

2014-04-17T14:23:30Z

134.76.70.252:

YybB

2014-04-17T14:23:28Z

134.76.70.252:

YyaF

2014-04-17T14:23:27Z

134.76.70.252:

YxxG

2014-04-17T14:23:23Z

134.76.70.252:

YxxD

2014-04-17T14:23:19Z

134.76.70.252:

YxjH

2014-04-17T14:23:17Z

134.76.70.252:

YxiG

2014-04-17T14:23:12Z

134.76.70.252:

YxiE

2014-04-17T14:23:12Z

134.76.70.252:

YxbD

2014-04-17T14:23:10Z

134.76.70.252:

YxbC

2014-04-17T14:23:05Z

134.76.70.252:

YxbB

2014-04-17T14:23:02Z

134.76.70.252:

YwtE

2014-04-17T14:22:59Z

134.76.70.252:

YwqG

2014-04-17T14:22:59Z

134.76.70.252:

SsbB

2014-04-17T14:22:58Z

134.76.70.252:

* '''Description:''' single-strand DNA-binding protein 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ssbB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywpH ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || single-strand DNA-binding protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || required for efficient genetic transformation
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36310 ssbB]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SsbB SsbB]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 6.876
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 339 bp, 113 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glcR]]'', ''[[ywpG]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36310 DNA_with_flanks]
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ywpH_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ssbB_3740206_3740547_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ssbB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36310]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[DNA replication]]}},
{{SubtiWiki category|[[genetic competence]]}},
{{SubtiWiki category|[[DNA repair/ recombination]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[ComK regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU36310

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36310&redirect=T BSU36310]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywpH-glcR.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12502]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** binds and protects internalized foreign ssDNA {{PubMed|22373918}}

* '''Protein family:'''

* '''Paralogous protein(s):''' [[SsbA]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:'''
** phosphorylated on Arg-55 {{PubMed|22517742}}
** phosphorylated on Tyr by [[PtkA]], dephosphorylated by [[PtpZ]] {{PubMed|16549871}}

* '''[[Cofactors]]:'''

* '''Effectors of protein activity:''' phosphorylation strongly increases DNA-binding activity {{PubMed|16549871}}

* '''[[SubtInteract|Interactions]]:'''
** forms tetramers {{PubMed|22373918}}
** [[SsbB]]-[[ComFA]] {{PubMed|17630974}}
** [[SsbB]]-[[CoiA]] {{PubMed|17630974}}
** [[SsbB]]-[[DprA]] {{PubMed|17630974}}
** [[SsbB]]-[[RecA]] {{PubMed|17630974}}

* '''[[Localization]]:'''
** localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
** localizes to the DNA entry pole during transformation {{PubMed|22373918}}

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36310&redirect=T BSU36310]

* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId= 3VDY 3VDY] (in complex with ssDNA) {{PubMed|22373918}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SPB6 C0SPB6]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36310]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:'''
** ''[[ssbB]]'' {{PubMed|22383849}}
** ''[[ssbB]]-[[glcR]]'' {{PubMed|14762004}} (this is unlikely according to {{PubMed|22383849}})

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ssbB_3740206_3740547_-1 ssbB] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
** expressed at the onset of stationary phase {{PubMed|14762004}}

* '''Regulatory mechanism:'''
** [[ComK]]: transcription activation {{PubMed|11948146}}

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 144 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 617 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 473 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1758 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
== Reviews ==
<pubmed> 22933559 </pubmed>
== Original publications ==
<pubmed>16009133,17630974, 22373918,16549871,14762004 11948146 22383849 22517742 23536821 23779106</pubmed>

[[Category:Protein-coding genes]]

YwpB

2014-04-17T14:22:53Z

134.76.70.252:

YwnH

2014-04-17T14:22:51Z

134.76.70.252:

* '''Description:''' similar to phosphinothricin acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ywnH''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36560 ywnH]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.277
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 489 bp, 163 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIQ]]'', ''[[ywnG]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36560 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ywnH_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywnH_3760694_3761185_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ywnH_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36560]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU36560

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36560&redirect=T BSU36560]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12486]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + phosphinothricin = CoA + N-acetylphosphinothricin (according to Swiss-Prot)

* '''Protein family:''' PAT/BAR subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36560&redirect=T BSU36560]

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1VHS 1VHS]

* '''UniProt:''' [http://www.uniprot.org/uniprot/P71043 P71043]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36560]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.183 2.3.1.183]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywnH_3760694_3761185_1 ywnH] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 158 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 445 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 568 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 442 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 465 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

[[Category:Protein-coding genes]]

MaeA

2014-04-17T14:22:50Z

134.76.70.252:

YwjH

2014-04-17T14:22:49Z

134.76.70.252:

YwjB

2014-04-17T14:22:46Z

134.76.70.252:

CysL

2014-04-17T14:22:44Z

134.76.70.252:

BacF

2014-04-17T14:22:42Z

134.76.70.252:

BacD

2014-04-17T14:22:40Z

134.76.70.252:

BacB

2014-04-17T14:22:36Z

134.76.70.252:

GlxA

2014-04-17T14:22:33Z

134.76.70.252:

* '''Description:''' glyoxalase I 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''glxA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-18r, ywbC ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glyoxalase I
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || detoxification of methylglyoxal
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38370 glxA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=ywbC glxA]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 4.429
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 378 bp, 126 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywbD]]'', ''[[ywbB]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38370 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:ywbC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywbC_3937135_3937515_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ywbC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38370]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38370

===Phenotypes of a mutant ===
* increased sensitivity to methylglyoxal {{PubMed|24330391}}

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywbC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10564]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''
** methylglyoxal + BSH --> S-lactoyl-bacillithiol (BSH) {{PubMed|24330391}}

* '''Protein family:''' glyoxalase I family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:'''

* '''[[Cofactors]]:'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39586 P39586]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38370]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''glxA'' {{PubMed|9353933}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywbC_3937135_3937515_1 glxA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 229 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3299 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1387 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2906 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>9353933 24330391 </pubmed>
[[Category:Protein-coding genes]]

YwaA

2014-04-17T14:22:31Z

134.76.70.252:

YvlB

2014-04-17T14:22:28Z

134.76.70.252:

CysJ

2014-04-17T14:22:26Z

134.76.70.252:

* '''Description:''' sulfite reductase (NADPH2) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''cysJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvgQ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || sulfite reductase (NADPH2)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || sulfite reduction
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440 cysJ]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cysJ cysJ]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.49
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1713 bp, 571 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nhaK]]'', ''[[cysI]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33440 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvgQ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:cysJ_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33440]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[sulfur metabolism]]}},
[[most abundant proteins]]

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CysL regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33430

===Phenotypes of a mutant ===
* unable to grow with sulfate or sulfite as the only sulfur source {{PubMed|12169591}}

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33440&redirect=T BSU33440]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvgRQ.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14099]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:'''

* '''[[Cofactors]]:''' contains an iron-sulfur cluster

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33440&redirect=T BSU33440]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O32214 O32214]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33430]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[cysI]]-[[cysJ]]'' {{PubMed|12169591}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysI_3430598_3432313_-1 cysJ] {{PubMed|22383849}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12169591}}

* '''Regulation:'''
** induced by sulfite, sulfate, and thiosulfate ([[CysL]]) {{PubMed|12169591}}
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]

* '''Regulatory mechanism:'''
** [[CysL]]: transcription activation {{PubMed|12169591}}

* '''Additional information:'''
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 2899 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1652 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1863 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 824 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 899 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''
** 1A802 ( ''cysJ''::''kan''), {{PubMed|11445163}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]
** 1A934 ( ''cysJ''::''kan''), {{PubMed|12169591}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>15378759,12169591,12107147, 11445163 </pubmed>

[[Category:Protein-coding genes]]

CysI

2014-04-17T14:22:23Z

134.76.70.252:

LutB

2014-04-17T14:22:21Z

134.76.70.252:

LutA

2014-04-17T14:22:19Z

134.76.70.252:

* '''Description:''' lactate catabolic enzyme 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''lutA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfV''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || lactate oxidase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || lactate utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34050 lutA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=lutA lutA]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 6.25
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 714 bp, 238 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lutB]]'', ''[[yvfU]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34050 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvfV_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvfV_3494985_3495701_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:lutA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34050]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[FbpB regulon]]}},
{{SubtiWiki regulon|[[FsrA regulon]]}},
{{SubtiWiki regulon|[[LutR regulon]]}},
{{SubtiWiki regulon|[[SinR regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU34050

===Phenotypes of a mutant ===

no growth with lactate as the single carbon source [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34050&redirect=T BSU34050]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12447]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' oxidation of lactate to pyruvate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:'''

* '''[[Cofactors]]:'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34050&redirect=T BSU34050]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07020 O07020]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34050]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[lutA]]-[[lutB]]-[[lutC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvfV_3494985_3495701_-1 lutA] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:''' induction by lactate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
** repressed by [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
** part of the iron sparing response, down-regulation in a'' [[fur]]'' mutant ([[Fur]], [[FsrA]], [[FpbB]]) {{PubMed|22427629}}

* '''Regulatory mechanism:''' dual repression by [[LutR]] (inducer lactate) and [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
** [[FsrA]]: translational repression {{PubMed|22427629}}
** [[FbpB]]: acts as RNA chaperone, increases interaction between ''[[fsrA]]'' and ''[[lutA]]-[[lutB]]-[[lutC]]'' RNAs {{PubMed|22427629}}

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 289 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 2918 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 799 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 738 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1342 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:''' GP1612 amyE::p(lutA-lacZ cat), constructed with pGP2149 based on [[pAC5]], available in [[ Jörg Stülke]]'s lab

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Richard Losick]], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

=Your additional remarks=

=References=

<pubmed>22427629,16430695,19201793 </pubmed>

[[Category:Protein-coding genes]]

YvdT

2014-04-17T14:22:17Z

134.76.70.252:

YvdF

2014-04-17T14:22:15Z

134.76.70.252:

YvcT

2014-04-17T14:22:13Z

134.76.70.252:

YvcN

2014-04-17T14:22:12Z

134.76.70.252:

* '''Description:''' unknown 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yvcN''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34730 yvcN]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.582
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 762 bp, 254 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvzJ]]'', ''[[crh]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34730 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34730 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34730 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvcN_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcN_3568527_3569291_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:yvcN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34730]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[proteins of unknown function]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU34730

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34730&redirect=T BSU34730]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12404]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' arylamine N-acetyltransferase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34730&redirect=T BSU34730]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O06977 O06977]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34730]

* '''E.C. number:'''

=== Additional information===
The gene is annotated in KEGG as an ortholog of EC 2.3.1.5 and is marked in Swiss-Prot as “uncharacterized acetyltransferase” (EC 2.3.1.-). No EC annotation is available in MetaCyc.No literature/ experimental evidence supporting the annotation is available. {{PubMed|19935659}}

=Expression and regulation=

* '''Operon:''' ''[[yvcI]]-[[yvcJ]]-[[yvcK]]-[[yvcL]]-[[crh]]-[[yvcN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9237995 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcN_3568527_3569291_-1 yvcN] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulation:''' very weak stimuation of expression by citrate and succinate [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 84 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 363 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 290 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 680 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>9237995 16272399, </pubmed>

[[Category:Protein-coding genes]]

YvcK

2014-04-17T14:22:10Z

134.76.70.252:

* '''Description:''' essential for growth under gluconeogenetic conditions, required for correct localization of [[ponA|PBP1]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yvcK''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || localization of [[ponA|PBP1]]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34760 yvcK]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 5.408
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 951 bp, 317 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcL]]'', ''[[yvcJ]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34760 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34760 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34760 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvcK_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcK_3570546_3571499_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:yvcK_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34760]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}}, {{SubtiWiki category|[[cell shape]]}}

= This gene is a member of the following [[regulons]] =
[[Efp-dependent proteins]]

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU34760

===Phenotypes of a mutant ===
* unable to grow with gluconeogenic substrates as single carbon source {{PubMed|16272399}}
* filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) {{PubMed|16272399}}, this is supressed by overexpression of [[MreB]] or by deletion of ''[[ponA]]'' ([[ponA|PBP1]] ) {{PubMed|21320184}}
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34760&redirect=T BSU34760]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12401]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' UPF0052 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:''' localized as a helical-like pattern {{PubMed|21320184}}

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34760&redirect=T BSU34760]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O06974 O06974]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34760]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yvcI]]-[[yvcJ]]-[[yvcK]]-[[yvcL]]-[[crh]]-[[yvcN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9237995 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcK_3570546_3571499_-1 yvcK] {{PubMed|22383849}}

* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulation:''' very weak stimuation of expression by citrate and succinate [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''
** [[translation]] is likely to require [[Efp]] due to the presence of several consecutive proline residues {{PubMed|23239624,23239623}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 96 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 246 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:''' pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab

* '''Antibody:'''

=Labs working on this gene/protein=
[[Boris Görke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]

[[Anne Galinier]], University of Marseille, France

=Your additional remarks=

=References=
==Reviews==
{{PubMed|21371139}}

==Original publications==
<pubmed>9237995 16272399, 21320184 </pubmed>
[[Category:Protein-coding genes]]

YvcI

2014-04-17T14:22:08Z

134.76.70.252:

* '''Description:''' [[Nudix hydrolase]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yvcI''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34780 yvcI]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 4.993
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 474 bp, 158 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcJ]]'', ''[[trxB]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34780 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34780 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34780 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvcI_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcI_3572413_3572889_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:yvcI_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34780]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[DNA repair/ recombination]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU34780

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34780&redirect=T BSU34780]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12399]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' [[Nudix hydrolase]]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU34780&redirect=T BSU34780]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O06972 O06972]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34780]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yvcI]]-[[yvcJ]]-[[yvcK]]-[[yvcL]]-[[crh]]-[[yvcN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9237995 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvcI_3572413_3572889_-1 yvcI] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulation:''' repressed by glucose (4.5-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed], very weak stimuation of expression by citrate and succinate [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]

* '''Regulatory mechanism:''' [[CcpA]]: transcription repression

* '''Additional information:'''
** A [[ncRNA]] is predicted between ''[[yvcI]]'' and ''[[trxB]]'' {{PubMed|20525796}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 67 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 601 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 472 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 816 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12850135 9237995 16272399, 20525796</pubmed>

[[Category:Protein-coding genes]]

LutC

2014-04-17T14:22:06Z

134.76.70.252:

YvbK

2014-04-17T14:22:04Z

134.76.70.252:

YvbH

2014-04-17T14:22:02Z

134.76.70.252:

AzoR2

2014-04-17T14:22:00Z

134.76.70.252:

IolW

2014-04-17T14:21:58Z

134.76.70.252:

* '''Description:''' scyllo-inositol dehydrogenase, general stress protein 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yvaA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || scyllo-inositol dehydrogenase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33530 iolW]
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.457
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1074 bp, 358 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[csoR]]'', ''[[azoR2]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33530 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33530 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33530 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yvaA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvaA_3444329_3445405_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:iolW_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33530]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SigB regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33530

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33530&redirect=T BSU33530]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14057]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' gfo/idh/mocA family (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[YfiI]], [[NtdC]], [[YrbE]], [[YteT]], [[YulF]], [[IolG]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU33530&redirect=T BSU33530]

* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3GFG 3GFG]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O32223 O32223]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33530]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yvaA_3444329_3445405_1 iolW] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]

* '''Regulation:'''
** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''
** number of protein molecules per cell (minimal medium with glucose and ammonium): 313 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1480 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5476 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3799 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 6429 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>15805528, 20133360 24325193 </pubmed>

[[Category:Protein-coding genes]]

SufA

2014-04-17T14:21:56Z

134.76.70.252:

YutJ

2014-04-17T14:21:54Z

134.76.70.252:

YutF

2014-04-17T14:21:52Z

134.76.70.252:

SufC

2014-04-17T14:21:50Z

134.76.70.252:

* '''Description:''' [[ABC transporter]] (ATP-binding protein), synthesis of Fe-S clusters 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yurY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sufC ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[ABC transporter]] (ATP-binding protein)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of Fe-S clusters
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32710 sufC]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=SufC SufC]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 4.621
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 783 bp, 261 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sufD]]'', ''[[yuzK]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32710 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yurY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sufC_3359995_3360780_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:sufC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32710]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[ABC transporters]]}},
{{SubtiWiki category|[[iron metabolism]]}},
{{SubtiWiki category|[[essential genes]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
[[most abundant proteins]]

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU32710

===Phenotypes of a mutant ===

essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32710&redirect=T BSU32710]

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14011]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' Ycf16 subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:''' phosphorylated on ser/ thr/ tyr [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed]

* '''[[Cofactors]]:'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32710&redirect=T BSU32710]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P80866 P80866]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32710]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[sufC]]-[[sufD]]-[[sufS]]-[[sufU]]-[[sufB]]'' {{PubMed|22383849}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sufC_3359995_3360780_-1 sufC] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 3638 {{PubMed|24696501}}
** number of protein molecules per cell (complex medium with amino acids, without glucose): 9447 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 18937 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 8637 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 7943 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>10092453, 20097860,16493705, 22383849 15378759</pubmed>

[[Category:Protein-coding genes]]

SufD

2014-04-17T14:21:48Z

134.76.70.252:

SufB

2014-04-17T14:21:46Z

134.76.70.252:

FrlB

2014-04-17T14:21:43Z

134.76.70.252:

* '''Description:''' fructoselysine-6-P-glycosidase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''frlB''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yurP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || fructoselysine-6-P-glycosidase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || metabolism of aminoacylated fructose
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32610 frlB]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=frlB frlB]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.442
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 984 bp, 328 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[frlO]]'', ''[[yurQ]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32610 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yurP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlB_3351110_3352096_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:frlB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32610]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
[[most abundant proteins]]

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CodY regulon]]}}, {{SubtiWiki regulon|[[FrlR regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU32610

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32610&redirect=T BSU32610]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yurRQPONML.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14002]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''[[Domains]]:'''

* '''Modification:'''
** phosphorylated on Arg-48 {{PubMed|22517742}}

* '''[[Cofactors]]:'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32610&redirect=T BSU32610]

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3EUA 3EUA]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O32157 O32157]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32610]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:'''
** ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|23175651,21347729}}
** ''[[frlB]]-[[frlO]]'' {{PubMed|23175651}}
** ''[[frlB]]'' {{PubMed|23175651}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlB_3351110_3352096_-1 frlB] {{PubMed|22383849}}

* '''[[Sigma factor]]:'''

* '''Regulation:'''
**'' [[frlB]]'': repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
** ''[[frlB]]'': induced in the presence of fructosamine ([[FrlR]]) {{PubMed|21397556}}

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1], [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]
** [[FrlR]]: transcription repression {{PubMed|21397556}}

* '''Additional information:'''
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 3695 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9758 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5339 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 6442 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed>22517742 23175651 21815947 15556630,18083814,12618455, 21347729, 18763711, 21398478 15378759</pubmed>

[[Category:Protein-coding genes]]

FrlD

2014-04-17T14:21:40Z

134.76.70.252:

* '''Description:''' fructosamine kinase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''frlD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yurL ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || fructosamine kinase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || metabolism of sugar amines
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32570 frlD]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=frlD frlD]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 4.909
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 852 bp, 284 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[frlR]]'', ''[[frlM]]''
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32570 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' [[Image:yurL_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlD_3347051_3347905_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:frlD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32570]]
|-
|}

__TOC__
 
 
 
 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[utilization of nitrogen sources other than amino acids]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CodY regulon]]}}, {{SubtiWiki regulon|[[FrlR regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU32570

===Phenotypes of a mutant ===

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32570&redirect=T BSU32570]

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yurRQPONML.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13998]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' carbohydrate kinase pfkB family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:'''

=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32570&redirect=T BSU32570]

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O32153 O32153]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32570]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:'''
** ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|23175651,21347729}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlD_3347051_3347905_-1 frlD] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''
**'' [[frlB]]'': repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
** ''[[frlB]]'': induced in the presence of fructosamine ([[FrlR]]) {{PubMed|21397556}}

* '''Regulatory mechanism:'''
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed1], [http://www.ncbi.nlm.nih.gov/sites/entrez/18083814 PubMed2]
** [[FrlR]]: transcription repression {{PubMed|21397556}}

* '''Additional information:'''
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
** number of protein molecules per cell (minimal medium with glucose and ammonium): 828 {{PubMed|24696501}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9042 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3514 {{PubMed|21395229}}
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4350 {{PubMed|21395229}}

=Biological materials =
* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed> 23175651 21815947 15556630,16153181, 21347729,12618455, 21398478</pubmed>

[[Category:Protein-coding genes]]

YurJ

2014-04-17T14:21:37Z

134.76.70.252: