manA
168
mannose-6-phosphate isomerase, required for proper cell wall synthesis
locus
BSU_12020
Molecular weight
35.85 kDa
pI
5.28
function
mannose utilization
product
mannose-6-phosphate isomerase
essential
no
synonyms
manA, yjdE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1482 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,274,692 → 1,275,639
Phenotypes of a mutant
cells lose the characteristic rod shape typical of wild type ''B. subtilis'' cells and instead appear as elongated spheres, which are significantly larger than normal [Pubmed|20862359]
The protein
Catalyzed reaction/ biological activity
D-mannose 6-phosphate --> D-fructose 6-phosphate (according to UniProt)
Protein family
mannose-6-phosphate isomerase type 1 family (with [protein|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF] and [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi], according to UniProt)
Structure
[PDB|1QWR] ([protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi], 56% identity)
[AF|O31646]
Paralogous protein(s)
[protein|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF], [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi]
Expression and Regulation
Operons
genes
[gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF]
description
[Pubmed|20139185]
regulation
induced by mannose ([protein|F273002AF97D87BB025B4F014C328C5592EAD621|manR]) [Pubmed|20139185]
expession may be controlled by a potential RNA switch located in the 5' untranslated region of the [gene|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]-[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]-[gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF] mRNA between [gene|E26C70893C5D677C816C814558CC42F90B920087|manA] and [gene|2DB0F763349D34FB1FCBBB4665416E129AD0AE6A|yjdF]
regulatory mechanism
[protein|F273002AF97D87BB025B4F014C328C5592EAD621|manR]: activation, in the presence of mannose and absence of glucose [Pubmed|20139185], in [regulon|protein:F273002AF97D87BB025B4F014C328C5592EAD621|manR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20139185], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A265 (yjdE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/265 NBRP B. subtilis, Japan]
BKE12020 (Δ[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCGTCATGAAAATCCCCC, downstream forward: _UP4_TAACATGGCAGGGCTTGAGA
BKK12020 (Δ[gene|E26C70893C5D677C816C814558CC42F90B920087|manA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTCGTCATGAAAATCCCCC, downstream forward: _UP4_TAACATGGCAGGGCTTGAGA
References
Reviews
Original Publications
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Time of last update: 2024-11-09 01:08:52
Author of last update: Jstuelk