glmR

glmR
168

accessory uridyltransferase, regulator of carbon partitioning between central metabolism and peptidoglycan biosynthesis, control of [wiki|cell shape], required for correct localization of [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]

locus
BSU_34760
Molecular weight
34.53 kDa
pI
5.41
Protein length
Gene length
function
regulation of carbon partitioning between central metabolism and peptidoglycan biosynthesis, localization of [protein|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]
product
activator of [protein|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] activity
essential
no
synonyms
glmR, yvcK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0391 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,570,546  3,571,499
Phenotypes of a mutant
unable to grow with gluconeogenic substrates as single carbon source, this can be suppressed by mutations in [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR], [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf], [gene|8D0885839CD60C05FB806BBF08AFA05225E83B12|pgpH], [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS], [gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI], upon overexpression of [gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] or by a point mutation in [protein|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] (G47S) that results in increased phosphoglucosamine mutase activity [Pubmed|31589605,30478337,30248093,16272399]
filamentous or L-shape-like aberrant morphologies (suppressed by Mg2+) [Pubmed|16272399], this is supressed by overexpression of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] or by deletion of [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1], [gene|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS], or [gene|CCD06F344E3C87E77C3D13883CE2B927F372A84E|yfnI] [Pubmed|30478337,21320184]
a [gene|70412A747D1ED3E885126945DF02503667C13E5B|pgcA] [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] double mutant is not viable, this can be suppressed by overexpression of [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM] [pubmed|31589605]
inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]
The protein
Catalyzed reaction/ biological activity
GlcNAc-1P + UTP --> UDP-N-acetylglucosamine [pubmed|36939339]
stimulates [protein|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] activity to facilitate the diversion of carbon from fructose-6-phosphate to peptidoglycan synthesis [pubmed|32994436,30248093]
interacts with [protein|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ] to facilitate genetic transformation [pubmed|32994436]
Protein family
gluconeogenesis factor family (single member, according to UniProt)
[wiki|Domains]
phosphorylated on Thr-304 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC] [Pubmed|25012659]
Structure
[PDB|2O2Z] (the protein from ''B. halodurans'', 63% identity, 86% similarity)
[AF|O06974]
Effectors of protein activity
binds UDP-GlcNAc [pubmed|28646159], this prevents the interaction with [protein|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] and stimulates interaction with [protein|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ] [pubmed|32994436,30248093]
[wiki|Localization]
localized as a helical-like pattern [Pubmed|25012659,21320184]
Expression and Regulation
Operons
genes
[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
description
[Pubmed|9237995]
regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) ([protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]) [pubmed|38512984]
regulatory mechanism
[protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]: transcription repression, described for Enterococcus faecalis [pubmed|38512984], in [regulon|protein:1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16272399], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]' [PubMed|20525796]
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[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]→[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]

2024-10-08 04:19:11

Jstuelk

130

BCC5699F6256B8A1B7FD8A979DBBBC9D4D1198AB

9244FB113E998572106D983A19AB3AC81B9F9D35

additional information
translation is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
MGNA-B640 (yvcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1639 NBRP B. subtilis, Japan]
BKE34760 (Δ[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC,  downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
BKK34760 (Δ[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC,  downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
Expression vectors
pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Boris Görke]'s lab
labs
[wiki|Anne Galinier], University of Marseille, France
References
Reviews
21371139,34248920,37238589
Original Publications
9237995,16272399,21320184,25012659,25047842,27806131,28646159,30248093,30478337,31589605,32994436,36939339,39185233

7150BABA5086D5E2EB8102E4901A216F43576282

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Time of last update: 2024-10-15 19:26:39

Author of last update: Jstuelk