Tue Dec 13 2016 11:16:29 GMT+0100 (CET)2025-05-11 17:57:39

locus

BSU00420

BSU_00420

outlinks

bsu

BSU00420

BSU_00420

aminos

MNKDIATPIRTKEILKKYGFSFKKSLGQNFLIDTNILNRIVDHAEVTEKTGVIEIGPGIGALTEQLAKRAKKVVAFEIDQRLLPILKDTLSPYENVTVIHQDVLKADVKSVIEEQFQDCDEIMVVANLPYYVTTPIIMKLLEEHLPLKGIVVMLQKEVAERMAADPSSKEYGSLSIAVQFYTEAKTVMIVPKTVFVPQPNVDSAVIRLILRDGPAVDVENESFFFQLIKASFAQRRKTLLNNLVNNLPEGKAQKSTIEQVLEETNIDGKRRGESLSIEEFAALSNGLYKALF

[SW|Categories] containing this gene/protein

[SW|translation]

Gene

Coordinates on the chromosome (coding sequence)

50,640 -> 51,518

The protein

Catalyzed reaction/ biological activity

methylation of A1518 and A1519 in 16S rRNA

adenosine¹⁵¹⁸/adenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-methionine --> 4 H⁺ + N⁶-dimethyladenosine¹⁵¹⁸/N⁶-dimethyladenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-homocysteine (according to UniProt)

The protein

Protein family

KsgA subfamily (according to Swiss-Prot)

[SW|Methyltransferase superfamily] (according to UniProt)

[SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

The protein

Structure

[PDB|3FUU] (from ''Thermus thermophilus'', 35% identity) [Pubmed|19285505]

[PDB|6IFT] (complexed with S-adenosylmethionine) [Pubmed|30624914]

[PDB|6IFS] [Pubmed|30624914]

[PDB|6IFV] (C-terminal truncation) [Pubmed|30624914]

[PDB|6IFW] (Chimeric protein) [Pubmed|30624914]

Expression and Regulation

Operon

''[[protein|rnmV]]-[[protein|ksgA]]'' [Pubmed|11233981]

References

16497325, 19001112, 11233981, 19285505

16497325, 19001112, 11233981, 19285505, 30624914

proteinLength

292

geneLength

879

Gene

Coordinates

50,640 → 51,518

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Localization]

cytoplasm (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B907 (ksgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1906 NBRP B. subtilis, Japan]

BKE00420 (Δ[[gene|ksgA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG

BKK00420 (Δ[[gene|ksgA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG