2017-8-11 16:0:32025-05-28 06:29:08
locus
BSU23580
BSU_23580
geneLength
987
990
outlinks
bsu
BSU23580
BSU_23580
The protein
Catalyzed reaction/ biological activity
L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
H2O + L-asparagine --> L-aspartate + NH4+ (according to UniProt)
The protein
Protein family
asparaginase 1 family (according to Swiss-Prot)
asparaginase 1 family (with [[protein|AnsZ]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|AnsZ]]
[[this]]
Biological materials
Mutant
GP1153 (del ''ansAB''::''ermC'') available in the [SW|Stülke] lab
GP1153 (Δ[[gene|ansA]]-[[gene|ansB]]::''ermC'') available in [SW|Jörg Stülke]'s lab
BKE23580 (Δ[[gene|ansA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
BKK23580 (Δ[[gene|ansA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC, downstream forward: _UP4_TAAAATGTAAAAACGATAAC
Biological materials
lacZ fusion
pGP872 (in [[protein|pAC5]]), available in [SW|Stülke] lab
pGP872 (in [SW|pAC5]), available in [SW|Stülke] lab
References
The protein
[SW|Domains]
Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)
The protein
Structure
[PDB|5OT0] (from Thermococcus kodakarensis, 43% identity) [pubmed|29095161]