description

transcriptional repressor of citZ and citB

transcriptional repressor of [[gene|citZ ]]and [[gene|citB]]

locus

BSU14140

BSU_14140

geneLength

879

882

product

transcriptional repressor (LysR family)

transcriptional repressor ([SW|LysR family])

ec

outlinks

bsu

BSU14140

BSU_14140

The protein

Protein family

[SW|LysR family]

[SW|LysR family] (according to UniProt)

Biological materials

Mutant

MGNA-A774 (ykuM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/774 NBRP B. subtilis, Japan]

GP206 (''aphA3'') & GP1442 (''aphA3'') , available in [SW|Stülke] lab

BKE14140 (Δ[[gene|ccpC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT

BKK14140 (Δ[[gene|ccpC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT

MGNA-A774 (ykuM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/774 NBRP B. subtilis, Japan]

GP206 (''aphA3'') & GP1442 (''aphA3'') , available in [SW|Jörg Stülke]'s lab

BKE14140 (Δ[[gene|ccpC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT

BKK14140 (Δ[[gene|ccpC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGTCCTCCTTTTTGT, downstream forward: _UP4_TAAAGACAGCGAGGTGCTGT

GP2800 (''Δ''''[[gene|darB]]-[[gene|ccpC]]]''::''phleo''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vector

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP706, available in [SW|Stülke] lab

Biological materials

lacZ fusion

pGP707 (in [[protein|pAC5]]), available in [SW|Stülke] lab, pGP711 (in [[protein|pAC6]]), available in [SW|Stülke] lab

pGP707 (in [SW|pAC5]), available in [SW|Stülke] lab, pGP711 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH lysR-type domain] (aa 1-58) (according to UniProt)

The protein

Structure

[PDB|5Z72] (from B. amyloliquefaciens, 96% identity)

The protein

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000024 PRODORIC2 database]

Biological materials

Expression vectors

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP706, available in [SW|Jörg Stülke]'s lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]