SubtiBank SubtiBank
Version comparison:

2018-07-17 19:15:132025-05-09 16:07:29

description

effector protein controlling CdaA diadenylate cyclase activity

effector protein controlling [[protein|CdaA]] diadenylate cyclase activity

locus

BSU01760

BSU_01760

geneLength

1449

1452

product

effector protein controlling CdaA diadenylate cyclase activity

effector protein controlling [[protein|CdaA]] diadenylate cyclase activity

ec

outlinks

bsu

BSU01760

BSU_01760

Biological materials

Expression vector

IPTG inducible expression of Strep-''cdaR'' in ''E. coli'': pGP2565 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

IPTG inducible expression of His-''cdaR'' in ''E. coli'': pGP2557 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab

GP2031: ''cdaR-Strep aphA3'', chromosomal expression in B. subtilis, for [[protein|SPINE]], available in [SW|Jörg Stülke]'s lab

References

Reviews

25869574

25869574, 30224435, 32603625

References

Original publications

12884008, 16236721, 26240071, 23192352, 22211522, 21154411, 26025048, 26527648, 27834680

32250026, 12884008, 16236721, 26240071, 23192352, 22211522, 21154411, 26025048, 26527648, 27834680, 32250026

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

YbbR-like 1 domain (aa 55-135) (according to UniProt)

YbbR-like 2 domain (aa 143-228) (according to UniProt)

YbbR-like 3 domain (aa 237-316) (according to UniProt)

YbbR-like 4 domain (aa 329-394) (according to UniProt)

Expression and Regulation

additional information

the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Expression vectors

IPTG inducible expression of Strep-''cdaR'' in ''E. coli'': pGP2565 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

IPTG inducible expression of His-''cdaR'' in ''E. coli'': pGP2557 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab

GP2031: ''cdaR-Strep aphA3'', chromosomal expression in B. subtilis, for [[protein|SPINE]], available in [SW|Jörg Stülke]'s lab