SubtiBank SubtiBank
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Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-23 01:00:28

description

mother cell-specific sporulation protein

mother cell-specific [SW|sporulation] protein, similar to 2-phosphosulfolactate phosphatase

locus

BSU10940

BSU_10940

ec

outlinks

bsu

BSU10940

BSU_10940

aminos

MPIAIYQGHHHSLAPADINIVIDVIRAFTVAHYAFIGGAKEILLVRTADEAFALKDTYPDYVLTGEEKGVGISGFDLDNSPKRMAGQNMTDKSLIQKTTNGVTAALGALNAKHLFVTGFSNAKTTAQHVKKLVANDCVINIVASHPSGDDDMACAEYIKGIIEGTNVVTAAEAIERIKGSSVAEKFFDCRQPLFDSEDIVYCTKELTGDFVMKVKQDGEVPTIERVII

[SW|Categories] containing this gene/protein

[SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,172,650 -> 1,173,336

Expression and Regulation

Operon

''[[protein|yitC]]-[[protein|yitD]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigE]] [Pubmed|15383836], [[protein|SigK]] [Pubmed|15699190,15383836]

Expression and Regulation

Regulation

expressed during [SW|sporulation] in the mother cell ([[protein|SigE]], [[protein|SigK]]) [Pubmed|15699190,15383836]

expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

References

15699190, 15383836, 27766092

15699190, 15383836, 27766092, 16927339

proteinLength

228

geneLength

687

Gene

Coordinates

1,172,650 → 1,173,336

The protein

Catalyzed reaction/ biological activity

(2R)-O-phospho-3-sulfolactate + H2O --> (R)-3-sulfolactate + phosphate (according to UniProt)

The protein

Protein family

ComB family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|1VR0] (from Clostridium acetobutylicum, 31% identity) [pubmed|16927339]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B177 (yitC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1176 NBRP B. subtilis, Japan]

BKE10940 (Δ[[gene|yitC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTACCTCTCCTTGGGC, downstream forward: _UP4_CCAACGATAGAGAGGGTCAT

BKK10940 (Δ[[gene|yitC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTACCTCTCCTTGGGC, downstream forward: _UP4_CCAACGATAGAGAGGGTCAT