Thu Aug 25 2016 17:06:39 GMT+0200 (CEST)2025-05-15 13:06:15
locus
BSU09990
BSU_09990
product
transcriptional repressor (MarR family)
transcriptional repressor ([SW|MarR family])
ec
outlinks
bsu
BSU09990
BSU_09990
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|transition state regulators], [SW|phosphoproteins]
Gene
Coordinates on the chromosome (coding sequence)
1,073,106 -> 1,073,717
The protein
Effectors of protein activity
activity is inhibited in an unknown way by [SW|PrkA] (possibly phosphorylation) [Pubmed|25983726]
activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]
DNA binding is affected by [[protein|DnmA]]-mediated DNA methylation [pubmed|32324221]
The protein
[SW|Interactions]
[[protein|SinR]]-[[protein|ScoC]]
Expression and Regulation
Operon
''scoC'' [Pubmed|3131303]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|19251843]
Expression and Regulation
Regulation
expressed upon transition into the stationary phase ([[protein|AbrB]]) [Pubmed|2504584]
Expression and Regulation
Regulatory mechanism
[[protein|AbrB]]: transcription activation [Pubmed|2504584]
[[protein|SenS]]: positive regulation [Pubmed|16321961]
[[protein|SalA]]: negative regulation [Pubmed|15126467]
[[protein|CodY]]: negative regulation [Pubmed|25966844]
Biological materials
Mutant
Strain OM213 of Ogura MA, et al [Pubmed|15126467], genotype ''trpC2 scoC::pMutin-scoC leuC7'', available as [http://bgsc.org BGSC] 1A918
1A918 ( ''scoC''::''erm''), [Pubmed|15126467], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A918&Search=1A918 BGSC]
1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]
BKE09990 (Δ[[gene|scoC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
BKK09990 (Δ[[gene|scoC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
Biological materials
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
References
proteinLength
203
geneLength
612
Gene
Coordinates
1,073,106 → 1,073,717
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|HTH marR-type domain] (aa 13-157) (according to UniProt)
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000050 PRODORIC2 database]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
References
Reviews
References
Original publications