SubtiBank SubtiBank
Version comparison:

Thu Aug 25 2016 17:06:39 GMT+0200 (CEST)2025-05-15 13:06:15

locus

BSU09990

BSU_09990

product

transcriptional repressor (MarR family)

transcriptional repressor ([SW|MarR family])

ec

outlinks

bsu

BSU09990

BSU_09990

[SW|Categories] containing this gene/protein

[SW|transcription factors and their control], [SW|transition state regulators], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

1,073,106 -> 1,073,717

The protein

Effectors of protein activity

activity is inhibited in an unknown way by [SW|PrkA] (possibly phosphorylation) [Pubmed|25983726]

activity is inhibited in an unknown way by [[protein|PrkA]] (possibly phosphorylation) [Pubmed|25983726]

DNA binding is affected by [[protein|DnmA]]-mediated DNA methylation [pubmed|32324221]

The protein

[SW|Interactions]

[[protein|SinR]]-[[protein|ScoC]]

Expression and Regulation

Operon

''scoC'' [Pubmed|3131303]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|19251843]

Expression and Regulation

Regulation

expressed upon transition into the stationary phase ([[protein|AbrB]]) [Pubmed|2504584]

Expression and Regulation

Regulatory mechanism

[[protein|AbrB]]: transcription activation [Pubmed|2504584]

[[protein|SenS]]: positive regulation [Pubmed|16321961]

[[protein|SalA]]: negative regulation [Pubmed|15126467]

[[protein|CodY]]: negative regulation [Pubmed|25966844]

Biological materials

Mutant

Strain OM213 of Ogura MA, et al [Pubmed|15126467], genotype ''trpC2 scoC::pMutin-scoC leuC7'', available as [http://bgsc.org BGSC] 1A918

1A918 ( ''scoC''::''erm''), [Pubmed|15126467], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A918&Search=1A918 BGSC]

1A918 ( ''scoC''::''erm trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]

BKE09990 (Δ[[gene|scoC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

BKK09990 (Δ[[gene|scoC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC, downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

Biological materials

Expression vector

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

References

3131303, 16923912, 1906467, 10383984, 16321961, 14629015, 1907636, 15126467, 2504584, 15104138, 19118355, 19801406, 19898538, 20382764, 22517742, 23660663, 11717292, 25966844, 19251843, 26473603, 25983726, 26728191

proteinLength

203

geneLength

612

Gene

Coordinates

1,073,106 → 1,073,717

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH marR-type domain] (aa 13-157) (according to UniProt)

The protein

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000050 PRODORIC2 database]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

References

Reviews

28886686

References

Original publications

3131303, 16923912, 1906467, 10383984, 16321961, 14629015, 1907636, 15126467, 2504584, 15104138, 19118355, 19801406, 19898538, 20382764, 22517742, 23660663, 11717292, 25966844, 19251843, 26473603, 25983726, 26728191, 31039790, 32324221