Sun Nov 27 2016 17:42:36 GMT+0100 (CET)2025-05-03 08:01:43
locus
BSU29180
BSU_29180
outlinks
bsu
BSU29180
BSU_29180
[SW|Categories] containing this gene/protein
[SW|ATP synthesis], [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
2,984,788 -> 2,986,545
Gene
Phenotypes of a mutant
unable to grow with non-[SW|PTS] carbohydrates (such as glucitol or glycerol) as single carbon source
suppression of ''[[protein|ftsZ]]''(ts) mutation (reverted by addition of pyruvate) [Pubmed|24825009]
unable to grow with non-[[protein|PtsI]] carbohydrates (such as glucitol or glycerol) as single carbon source
suppression of ''[[gene|ftsZ]]''(ts) mutation (reverted by addition of pyruvate) [Pubmed|24825009]
The protein
Catalyzed reaction/ biological activity
ADP
The reaction is irreversible under physiological conditions
ADP + phosphoenolpyruvate → ATP + pyruvate
The reaction is irreversible under physiological conditions
The protein
Protein family
PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
C-terminal part: [SW|PEP-utilizing enzyme family] (according to UniProt)
pyruvate kinase family (single member, according to UniProt)
The protein
[SW|Cofactors]
Mg2
Mg2+, K+
The protein
Structure
[PDB|2E28] (Geobacillus stearothermophilus)
[PDB|2E28] (Geobacillus stearothermophilus) [pubmed|18511452]
The protein
Additional information
The enzyme is a tetramer with four active sites [Pubmed|3711058]
The enzyme is a tetramer with four active sites [Pubmed|3711058]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operon
''[[protein|pfkA]]-[[protein|pyk]]-[[protein|ytzA]]'' [Pubmed|11489127]
Expression and Regulation
Regulation
twofold induced by glucose [Pubmed|11489127]
Expression and Regulation
Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Biological materials
Mutant
GP589 (''pyk''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP600 (''pyk''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP1745: ''pyk::aphA3'', available in [SW|Jörg Stülke]' lab
GP589 (''pyk''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP600 (''pyk''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
GP1745: BSB1 ''pyk::aphA3'', available in [SW|Jörg Stülke]' lab
BKE29180 (Δ[[gene|pyk]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTTCACTTCCTTCT, downstream forward: _UP4_TAATTACAGGTGAAAATGGA
BKK29180 (Δ[[gene|pyk]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTTCACTTCCTTCT, downstream forward: _UP4_TAATTACAGGTGAAAATGGA
Biological materials
Expression vector
expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
expression in ''B. subtilis'', native protein: pGP1411 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
see ''[[protein|pfkA]]
see ''[[gene|pfkA]]
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]
Labs working on this gene/protein
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
proteinLength
585
geneLength
1758
Gene
Coordinates
2,984,788 → 2,986,545
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
expression in ''B. subtilis'', native protein: pGP1411 (in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]