2017-05-24 08:37:252025-05-14 20:26:27
description
N-terminal part of the split gene spsM (together with ypqP), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage
5' part of the split gene [[gene|ypqP]] (together with [[gene|ypqP]]), legionaminic acid synthesis, in B. subtilis 168 the gene is disrupted by the [[category|SP-beta prophage]]
locus
BSU19810
BSU_19810
proteinLength
141
139
geneLength
423
420
function
spore envelope polysaccharide biosynthesis
legionaminic acid synthesis
product
unknown
UDP-NAcGlcA inverting 4,6-dehydratase (N-terminal part)
ec
outlinks
bsu
BSU19810
BSU_19810
The protein
Paralogous protein(s)
[[protein|EpsC]] (for SpsM)
[[this]]
Biological materials
Mutant
MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]
MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]
BKE19810 (Δ[[gene|yodU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA
BKK19810 (Δ[[gene|yodU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA
References
Gene
Phenotypes of a mutant
the [[gene|ypqP]] mutant of PY79 has a less hydrophobic spore surface [pubmed|32817102]
The protein
Catalyzed reaction/ biological activity
SpsM catalyzes the C-4/C-6 dehydration of the UDP-GlcNAc to produce UDP-4-keto-6-deoxy-GlcNAc, this is the first reaction of the legionaminic acid biosynthesis pathway [pubmed|32817102]
required for spore crust assembly, legionaminic acid synthesis [pubmed|32817102]
The protein
Structure
[PDB|2GN4] (from Helicobacter pylori, 46% identity) [pubmed|16651261]