SubtiBank SubtiBank
Version comparison:

2017-05-24 08:37:252025-05-14 20:26:27

description

N-terminal part of the split gene spsM (together with ypqP), spore envelope polysaccharide biosynthesis, in B. subtilis 168 the gene is disrupted by the SP-beta prophage

5' part of the split gene [[gene|ypqP]] (together with [[gene|ypqP]]), legionaminic acid synthesis, in B. subtilis 168 the gene is disrupted by the [[category|SP-beta prophage]]

locus

BSU19810

BSU_19810

proteinLength

141

139

geneLength

423

420

function

spore envelope polysaccharide biosynthesis

legionaminic acid synthesis

product

unknown

UDP-NAcGlcA inverting 4,6-dehydratase (N-terminal part)

ec

outlinks

bsu

BSU19810

BSU_19810

The protein

Paralogous protein(s)

[[protein|EpsC]] (for SpsM)

[[this]]

Biological materials

Mutant

MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]

MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]

BKE19810 (Δ[[gene|yodU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA

BKK19810 (Δ[[gene|yodU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC, downstream forward: _UP4_GTATATTAAGATACTTACTA

References

25299644, 15383836, 25326298

25299644, 15383836, 25326298, 28535266, 16651261, 32817102

Gene

Phenotypes of a mutant

the [[gene|ypqP]] mutant of PY79 has a less hydrophobic spore surface [pubmed|32817102]

The protein

Catalyzed reaction/ biological activity

SpsM catalyzes the C-4/C-6 dehydration of the UDP-GlcNAc to produce UDP-4-keto-6-deoxy-GlcNAc, this is the first reaction of the legionaminic acid biosynthesis pathway [pubmed|32817102]

required for spore crust assembly, legionaminic acid synthesis [pubmed|32817102]

The protein

Structure

[PDB|2GN4] (from Helicobacter pylori, 46% identity) [pubmed|16651261]