SubtiBank SubtiBank
Version comparison:

Sun Oct 25 2015 18:14:12 GMT+0100 (CET)2025-05-09 14:24:03

locus

BSU23260

BSU_23260

outlinks

bsu

BSU23260

BSU_23260

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors]

The protein

Catalyzed reaction/ biological activity

D-ribulose 5-phosphate = formate L-3,4-dihydroxybutan-2-one 4-phosphate (according to Swiss-Prot)

D-ribulose 5-phosphate --> (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H+ (according to UniProt)

GTP + 3 H2O --> 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate + formate + 2 H+ (according to UniProt)

Expression and Regulation

Operon

''[[protein|ribD]]-[[protein|ribE]]-[[protein|ribA]]-[[protein|ribH]]-[[protein|ribT]]'' [Pubmed|8159171]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|8159171]

Expression and Regulation

Regulation

expressed in the absence of FMN ([[protein|FMN-box]]) [Pubmed|15808508]

Expression and Regulation

Regulatory mechanism

[[protein|FMN-box]]: [SW|riboswitch], mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination [Pubmed|15808508]

References

19583770, 12456892, 15808508, 8159171, 7934829, 7934830, 16115872, 23270261, 24442413, 26280801

19583770, 12456892, 15808508, 8159171, 7934829, 7934830, 16115872, 23270261, 24442413, 26280801, 26494285

proteinLength

398

geneLength

1197

Gene

Coordinates

2,428,389 → 2,429,585

The protein

Protein family

N-terminal part: DHBP synthase family (single member, according to UniProt)

C-terminal part: GTP cyclohydrolase II family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE23260 (Δ[[gene|ribA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGAAACATGCAAATCTTCC, downstream forward: _UP4_TAATCACAAATATCACAAAA

BKK23260 (Δ[[gene|ribA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGAAACATGCAAATCTTCC, downstream forward: _UP4_TAATCACAAATATCACAAAA