Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-06-01 21:17:39
locus
BSU07350
BSU_07350
ec
outlinks
bsu
BSU07350
BSU_07350
aminos
MFQYEELNKQFIGGKWQEGSSPNVLENKNPYTQKTFTTFRKATADDVDEAYRAAALAKKKWDAVNPFEKRTILEKAVTYIEENEEAIIYLIMEELGGTRLKAAFEIGLVKNIIKEAATFPIRMEGKILPSTIDGKENRLYRVPAGVVGVISPFNFPFFLSMKSVAPALGAGNGVVLKPHEETPICGGTLIAKIFENAGIPAGLLNVVVTDIAEIGDSFVEHPVPRIISFTGSTKVGSYIGQLAMKHFKKPLLELGGNSAFIVLEDADIEYAVNAAVFSRFTHQGQICMSANRVLVHSSIYDKFLELYQAKVESLKVGDPMDPDTIIGPLINSRQTDGLMKTVEQAIEEGAVPVKLGGFNGTIVEPTILKDVKPFMSIAKEELFGPVVSFMKFDSEDEAVDIANETPFGLSGAVHTSNLERGVAFAKRIETGMIHVNDTTINDEPNVAFGGEKQSGLGRLNGEWSLEEFTTLKWISVQHEKRSFPY
[SW|Categories] containing this gene/protein
[SW|poorly characterized/ putative enzymes]
Gene
Coordinates on the chromosome (coding sequence)
807,091 -> 808,548
The protein
Catalyzed reaction/ biological activity
oxidation of vanillin to vanillic acid [Pubmed|26658822]
oxidation of vanillin to vanillic acid [Pubmed|26658822]
benzaldehyde + H2O + NAD+ --> benzoate + 2 H+ + NADH (according to UniProt)
4-hydroxy-3-methoxybenzaldehyde + H2O + NAD+ --> 4-hydroxy-3-methoxybenzoate + 2 H+ + NADH (according to UniProt)
The protein
Paralogous protein(s)
[[protein|AldY]], [[protein|YcbD]], [[protein|GabD]], [[protein|GbsA]], [[protein|DhaS]], [[protein|IolA]], [[protein|PutC]], [[protein|RocA]], [[protein|AldX]], [[protein|YwdH]]
[[this]]
The protein
Structure
[PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 32% identity, 67% similarity)
[PDB|4DNG] (from ''Bacillus Subtilis'', 47% identity)
[PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 32% identity, 67% similarity)
Expression and Regulation
Operon
''[[protein|yfmT]]-[[protein|yfmS]]'' [Pubmed|9141694,15033535]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigD]] [Pubmed|15033535]
Labs working on this gene/protein
[SW|Josef Altenbuchner]
proteinLength
485
geneLength
1458
Gene
Coordinates
807,091 → 808,548
The protein
Protein family
[SW|aldehyde dehydrogenase family] (according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-C232 (yfmT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2230 NBRP B. subtilis, Japan]
BKE07350 (Δ[[gene|yfmT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
BKK07350 (Δ[[gene|yfmT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
labs
[SW|Josef Altenbuchner]