SubtiBank SubtiBank
Version comparison:

2017-02-27 15:02:042025-05-10 11:03:41

description

antagonist of biofilm repression by SinR, control of the phosphorelay

subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex, required for [[protein|Rny|RNase Y]] dependent maturation of polycistronic mRNAs, antagonist of biofilm repression by [[protein|SinR]], control of the [SW|phosphorelay]

locus

BSU17020

BSU_17020

geneLength

429

432

function

control of biofilm formation and the phosphorelay

control of [[protein|Rny|RNase Y]] activity, see [SW|Targets of the Y complex]

product

antagonist of biofilm repression by SinR

subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex

ec

names

ymcA

ymcA

ricA

outlinks

bsu

BSU17020

BSU_17020

Gene

Coordinates on the chromosome (coding sequence)

1,774,374 -> 1,774,805

Gene

Phenotypes of a mutant

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

The protein

Structure

[PDB|2PIH]

[PDB|6PRH] (the [[protein|YmcA]]dimer) [pubmed|31530674]

[PDB|6PRK] (the [[protein|YmcA]]-[[protein|YlbF]] complex) [pubmed|31530674]

Expression and Regulation

[[this]]

References

15661000, 23490197, 15175311, 26434553

Gene

Coordinates

1,774,374 → 1,774,805

The protein

Catalyzed reaction/ biological activity

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [[protein|YlbF]] and [[protein|YaaT]])) [pubmed|31530674]

The protein

[SW|Localization]

cytoplasm [pubmed|27501195]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE17020 (Δ[[gene|ymcA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17020 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_GTCTTTTTTTGAGTAGAGCG, downstream forward: _UP4_TAAACACGGTGCCTTTACAG

BKK17020 (Δ[[gene|ymcA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTTTTTTTGAGTAGAGCG, downstream forward: _UP4_TAAACACGGTGCCTTTACAG

References

Reviews

32061882, 32156829, 32156813

References

Original publications

15661000, 23490197, 15175311, 26434553, 28295778, 27501195, 29794222, 30265683, 31530674