Fri Dec 09 2016 18:28:56 GMT+0100 (CET)2025-05-13 03:10:16
description
Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts
Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[protein|RecA]] activities, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU22310
BSU_22310
function
DNA repair/ recombination and chromosome segregation
[SW|DNA repair/ recombination] and [SW|chromosome segregation]
ec
names
recU
recG
prfA
yppB
jopB
recU
prfA
yppB
jopB
outlinks
bsu
BSU22310
BSU_22310
[SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
Gene
Coordinates on the chromosome (coding sequence)
2,340,802 -> 2,341,422
Gene
Phenotypes of a mutant
the inactivations of ''[[protein|recU]]'' and ''[[protein|ruvA]]-[[protein|ruvB]]'' are synthetically lethal [Pubmed|24770420]
the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]
loss of plasmid transformation, this can be suppressed by deletion of [[gene|recA]] [pubmed|30050509]
The protein
Catalyzed reaction/ biological activity
binds Holliday junctions with high affinity [Pubmed|24770420]
binds Holliday junctions with high affinity [Pubmed|24770420]
inhibits [[protein|RecA]] filament growth, facilitates [[protein|RecA]] disassembly from preformed filaments [pubmed|30050509]
endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction) (according to UniProt)
promotes a net [[protein|RecA]] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]
The protein
[SW|Interactions]
form dimers [Pubmed|21600217]
[[protein|RecA]]-[[protein|RecU]] [Pubmed|16024744]
[[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420,21600217]
formation of a ternary complex [[protein|RecU]]-[[protein|RuvB]]-Holliday junction DNA [Pubmed|24770420]
Expression and Regulation
Operon
''[[protein|recU]]-[[protein|ponA]]'' [Pubmed|7814321]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigM]] [Pubmed|18179421]
Biological materials
Mutant
GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab
1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab
BKE22310 ( ''recU''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]
BP784 ( ''recU''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
BKE22310 (Δ[[gene|recU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
BKK22310 (Δ[[gene|recU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
References
Original publications
proteinLength
206
geneLength
621
Gene
Coordinates
2,340,802 → 2,341,422
The protein
Protein family
RecU family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]