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Fri Dec 09 2016 18:28:56 GMT+0100 (CET)2025-05-13 03:10:16

description

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [[protein|RecA]] activities, required for efficient survival and replication restart after replication-transcription conflicts

locus

BSU22310

BSU_22310

function

DNA repair/ recombination and chromosome segregation

[SW|DNA repair/ recombination] and [SW|chromosome segregation]

ec

names

recU

recG

prfA

yppB

jopB

recU

prfA

yppB

jopB

outlinks

bsu

BSU22310

BSU_22310

[SW|Categories] containing this gene/protein

[SW|DNA repair/ recombination], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates on the chromosome (coding sequence)

2,340,802 -> 2,341,422

Gene

Phenotypes of a mutant

the inactivations of ''[[protein|recU]]'' and ''[[protein|ruvA]]-[[protein|ruvB]]'' are synthetically lethal [Pubmed|24770420]

the inactivations of ''[[gene|recU]]'' and ''[[gene|ruvA]]-[[gene|ruvB]]'' are synthetically lethal [Pubmed|24770420]

loss of plasmid transformation, this can be suppressed by deletion of [[gene|recA]] [pubmed|30050509]

The protein

Catalyzed reaction/ biological activity

binds Holliday junctions with high affinity [Pubmed|24770420]

binds Holliday junctions with high affinity [Pubmed|24770420]

inhibits [[protein|RecA]] filament growth, facilitates [[protein|RecA]] disassembly from preformed filaments [pubmed|30050509]

endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction) (according to UniProt)

promotes a net [[protein|RecA]] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]

The protein

[SW|Interactions]

form dimers [Pubmed|21600217]

[[protein|RecA]]-[[protein|RecU]] [Pubmed|16024744]

[[protein|RecU]]-[[protein|RuvB]] [Pubmed|24770420,21600217]

formation of a ternary complex [[protein|RecU]]-[[protein|RuvB]]-Holliday junction DNA [Pubmed|24770420]

Expression and Regulation

Operon

''[[protein|recU]]-[[protein|ponA]]'' [Pubmed|7814321]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigM]] [Pubmed|18179421]

Biological materials

Mutant

GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab

1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]

GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab

BKE22310 ( ''recU''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]

BP784 ( ''recU''::''ermC''), (available in [SW|Fabian Commichau]'s lab)

1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]

BKE22310 (Δ[[gene|recU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA

BKK22310 (Δ[[gene|recU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA

References

Original publications

9642195, 16024744, 15317759, 11810266, 19730681, 19422832, 7814321, 18179421, 16154091, 21600217, 18684995, 16020779, 24770420, 24792171, 14701911, 25939832, 27903910

9642195, 16024744, 15317759, 11810266, 19730681, 19422832, 7814321, 18179421, 16154091, 21600217, 18684995, 16020779, 24770420, 24792171, 14701911, 25939832, 27903910, 30050509, 31876108

proteinLength

206

geneLength

621

Gene

Coordinates

2,340,802 → 2,341,422

The protein

Protein family

RecU family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]