SubtiBank SubtiBank
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Tue Dec 06 2016 16:21:43 GMT+0100 (CET)2025-05-25 14:38:25

description

heat-shock protein (activation of DnaK)

heat-shock protein (activation of [[protein|DnaK]])

locus

BSU25480

BSU_25480

function

control of DnaK activity

control of [[protein|DnaK]] activity

ec

outlinks

bsu

BSU25480

BSU_25480

[SW|Categories] containing this gene/protein

[SW|chaperones/ protein folding], [SW|heat shock proteins]

Gene

Coordinates on the chromosome (coding sequence)

2,627,971 -> 2,628,534

The protein

Protein family

grpE family (according to Swiss-Prot)

grpE family (single member, according to UniProt)

The protein

Structure

[PDB|4ANI] ([SW|DnaK]-[SW|GrpE] complex from ''Geobacillus kaustophilus'', 70% identity) [Pubmed|22544739]

[PDB|4ANI] ([[protein|DnaK]]-[[protein|GrpE]] complex from ''Geobacillus kaustophilus'', 70% identity) [Pubmed|22544739]

Expression and Regulation

Operon

''[[protein|hrcA]]-[[protein|grpE]]-[[protein|dnaK]]-[[protein|dnaJ]]-[[protein|yqeT]]-[[protein|yqeU]]-[[protein|yqeV]]'' [Pubmed|9023197]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|1339421]

Expression and Regulation

Regulation

induced by heat shock ([[protein|HrcA]]) [Pubmed|1339421]

Expression and Regulation

Regulatory mechanism

[[protein|HrcA]]: transcription repression [Pubmed|1339421]

Labs working on this gene/protein

[SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]

proteinLength

187

geneLength

564

Gene

Coordinates

2,627,971 → 2,628,534

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE25480 (Δ[[gene|grpE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCACCTCCCTCA, downstream forward: _UP4_TAATTACATAGCAGGAGGTT

BKK25480 (Δ[[gene|grpE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCACCTCCCTCA, downstream forward: _UP4_TAATTACATAGCAGGAGGTT

labs

[SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]