SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:53:20 GMT+0200 (CEST)2025-05-22 12:56:21

locus

BSU09290

BSU_09290

outlinks

bsu

BSU09290

BSU_09290

syns

[SW|Categories] containing this gene/protein

[SW|utilization of specific carbon sources], [SW|phosphoproteins]

The protein

Catalyzed reaction/ biological activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate (according to Swiss-Prot)

ATP + glycerol --> ADP + H+ + sn-glycerol 3-phosphate (according to UniProt)

The protein

Protein family

FGGY kinase family (according to Swiss-Prot)

[SW|FGGY kinase family] (according to UniProt)

The protein

[SW|Interactions]

[[protein|PtsH]]-[[protein|GlpK]]

Expression and Regulation

Operon

''[[protein|glpF]]-[[protein|glpK]]'' [Pubmed|2127799]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|2127799]

Expression and Regulation

Regulation

repressed by glucose (8-fold) ([[protein|CcpA]]) [Pubmed|12850135]

induction by glycerol ([[protein|GlpP]]) [Pubmed|8436953]

Expression and Regulation

Regulatory mechanism

[[protein|CcpA]]: transcription repression [Pubmed|12850135]

[[protein|GlpP]]-dependent [SW|RNA switch] (transcriptional antitermination) [Pubmed|8436953]

[[protein|AbrB]]: transcription activation [Pubmed|20817675]

Biological materials

Mutant

BKE09290 (''glpK''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab)

GP1865 (''glpK''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

BKE09290 (''glpK''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

GP1865 (''glpK''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

BKE09290 (Δ[[gene|glpK]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCATAAGATGTCTCCCCT, downstream forward: _UP4_TAAAGTAATACTATGGTATA

BKK09290 (Δ[[gene|glpK]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCATAAGATGTCTCCCCT, downstream forward: _UP4_TAAAGTAATACTATGGTATA

Labs working on this gene/protein

[SW|Josef Deutscher], Paris-Grignon, France

References

1335945, 9162046, 11929549, 7773413, 19102629, 20817675

1335945, 9162046, 11929549, 7773413, 19102629, 20817675, 28439033, 10913079, 28458661

proteinLength

496

geneLength

1491

Gene

Coordinates

1,003,344 → 1,004,834

The protein

Paralogous protein(s)

[[this]]

The protein

Modification

phosphorylation (His230) (according to Swiss-Prot)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

labs

[SW|Josef Deutscher], Paris-Grignon, France