Tue Dec 13 2016 11:16:30 GMT+0100 (CET)2025-05-09 06:40:04
description
transcriptional activator (AraC family), (AraC family DNA-binding domain fused to FeuA-like substrate-binding domain), regulation of the feuA-feuB-feuC-ybbA operon
transcriptional activator ([SW|AraC family]), (AraC family DNA-binding domain fused to [[protein|FeuA]]-like substrate-binding domain), regulation of the [[gene|feuA]]-[[gene|feuB]]-[[gene|feuC]]-[[gene|ybbA]] operon
locus
BSU01640
BSU_01640
product
transcription activator (AraC family)
transcription activator ([SW|AraC family])
ec
outlinks
bsu
BSU01640
BSU_01640
aminos
MQNAVIYQPVQIEYLKKTSDLFSEQQLADSFVLIFHLKGNGYISIGTNTNPLQKKTLYVCPPNETFGFTPAADGHIDACIIRLLSYIKETGQDIFTPCTESELAKLKLMNVSHIENLAVRLQELAALWNESSQLSQLKCVIEVQSLIYDLFTASLSDQTDTHSAIEKTKHYIETHADTKITLAQLSQMAGISAKHYSESFKKWTGQSVTEFITKTRITKAKRLMAKSNCKLKEIAHQTGYQDEFYFSRIFKKYTGCSPTSYMKKRRKKIAAYGRGTMGHLIPLHHIPFAAALHPKWTSYYYQHYSTDIPVQLSAYRFNEKWEENLYTLSQAEPDVIVSMDSISPEEQDRLNRIAEVMYLPSEESWRTHFLQTASFLKEESEAEKWLADYDQQTTAAKKTLQHVQGLRFLFLRLHKQNFYLAHNRSVREVFFGDLGFSSATTADTPSEQAISLENIANYQADCMMLFLFKEPETIAYYQQLQQTEAWQNLSAVRDNRVYLLSLDPWNEYSACGHERIVQQTVSLLSGDCP
[SW|Categories] containing this gene/protein
[SW|acquisition of iron], [SW|iron metabolism], [SW|transcription factors and their control]
Gene
Coordinates on the chromosome (coding sequence)
183,414 -> 185,003
The protein
Catalyzed reaction/ biological activity
binding of a direct repeat in the ''[[protein|feuA]]'' promoter region and transcription activation of the ''[[protein|feuA]]-[[protein|feuB]]-[[protein|feuC]]-[[protein|ybbA]]'' operon in the presence of bacillibactin or enterobactin [Pubmed|17725565,22210890]
binding of a direct repeat in the [[gene|feuA]] promoter region and transcription activation of the [[gene|feuA]]-[[gene|feuB]]-[[gene|feuC]]-[[gene|ybbA]] operon in the presence of bacillibactin or enterobactin [Pubmed|17725565,22210890]
The protein
[SW|Interactions]
[[protein|Btr]]-[SW|RNA polymerase] (perhaps [[protein|SigA]]) [Pubmed|22210890]
Expression and Regulation
Operon
''btr'' [Pubmed|12354229]
Expression and Regulation
Regulation
expressed under conditions of iron starvation ([[protein|Fur]]) [Pubmed|12354229]
Expression and Regulation
Regulatory mechanism
[[protein|Fur]]: transcription repression [Pubmed|12354229]
Expression and Regulation
Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
proteinLength
529
geneLength
1590
Gene
Coordinates
183,414 → 185,003
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Fe/B12 periplasmic-binding domain] (aa 268-528) (according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B949 (ybbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1948 NBRP B. subtilis, Japan]
BKE01640 (Δ[[gene|btr]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC, downstream forward: _UP4_TGATAAATCAGGAATTTGTC
BKK01640 (Δ[[gene|btr]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC, downstream forward: _UP4_TGATAAATCAGGAATTTGTC