SubtiBank SubtiBank
Version comparison:

Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-23 20:16:49

locus

BSU14840

BSU_14840

ec

outlinks

bsu

BSU14840

BSU_14840

[SW|Categories] containing this gene/protein

[SW|essential genes], [SW|proteins of unknown function]

Gene

Coordinates on the chromosome (coding sequence)

1,552,412 -> 1,552,693

Gene

Phenotypes of a mutant

essential [Pubmed|12682299]

essential [Pubmed|12682299], the mutant is viable if iron(III) is added to the medium [Pubmed|27238023]

The protein

Protein family

UPF0358 family (according to Swiss-Prot)

UPF0358 family (single member, according to UniProt)

Expression and Regulation

Operon

''[[protein|ylaN]]'' [Pubmed|22383849]

Biological materials

Expression vector

IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

17005971, 17469204, 22383849, 23420519

17005971, 17469204, 22383849, 23420519, 27238023, 28189581, 32949815

proteinLength

93

geneLength

282

Gene

Coordinates

1,552,412 → 1,552,693

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE14840 (Δ[[gene|ylaN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACT

BKK14840 (Δ[[gene|ylaN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC, downstream forward: _UP4_TAAATGATAAAACTCAAACT

Biological materials

Expression vectors

IPTG inducible expression of Strep-''ylaN'' in ''E. coli'': pGP2579 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

IPTG inducible expression of His-''ylaN'' in ''E. coli'': pGP2583 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab