Mon Feb 01 2016 16:12:50 GMT+0100 (CET)2025-05-24 04:15:25
locus
BSU15540
BSU_15540
outlinks
bsu
BSU15540
BSU_15540
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides]
Gene
Coordinates on the chromosome (coding sequence)
1,627,718 -> 1,628,653
The protein
Catalyzed reaction/ biological activity
(S)-dihydroorotate
(S)-dihydroorotate + NAD+ --> H+ + NADH + orotate (according to UniProt)
The protein
Protein family
Type 1 subfamily (according to Swiss-Prot)
dihydroorotate dehydrogenase family (single member, according to UniProt)
The protein
Structure
[PDB|1EP1] (from ''Lactococcus lactis'', 68% identity)
[PDB|1EP1] (from ''Lactococcus lactis'', 68% identity) [Pubmed|11188687]
Expression and Regulation
Operon
''[[protein|pyrR]]-[[protein|pyrP]]-[[protein|pyrB]]-[[protein|pyrC]]-[SW|pyrAA]-[SW|pyrAB]-[[protein|pyrK]]-[[protein|pyrD]]-[[protein|pyrF]]-[[protein|pyrE]]'' [Pubmed|8206849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|1709162]
Expression and Regulation
Regulation
induced in the absence of uridine nucleotides ([[protein|PyrR]]) [Pubmed|8206849]
Expression and Regulation
Regulatory mechanism
[[protein|PyrR]]: [SW|RNA switch], transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) [Pubmed|8955403]
Biological materials
Mutant
1A806 ( ''pyrD''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A806&Search=1A806 BGSC]
1A807 ( ''pyrD''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A807&Search=1A807 BGSC]
1A806 ( ''pyrD''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A806&Search=1A806 BGSC]
1A807 ( ''pyrD''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A807&Search=1A807 BGSC]
BKE15540 (Δ[[gene|pyrD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA, downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
BKK15540 (Δ[[gene|pyrD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCCGGCAATTTCACCTCTA, downstream forward: _UP4_GAATGCATCGGAAGGAGCTG
References
proteinLength
311
geneLength
936
Gene
Coordinates
1,627,718 → 1,628,653
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Cofactors]
FMN [pubmed|11188687]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]