SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-27 22:24:06

locus

BSU10930

BSU_10930

outlinks

bsu

BSU10930

BSU_10930

aminos

MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYELIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVNLDQKFELIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE

[SW|Categories] containing this gene/protein

[SW|sulfur metabolism], [SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,171,755 -> 1,172,465

The protein

Catalyzed reaction/ biological activity

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)

[thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H+ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)

The protein

Protein family

CysH subfamily (according to Swiss-Prot)

PAPS reductase family (with [[protein|CysH]], according to UniProt)

The protein

Paralogous protein(s)

[[protein|CysH]]

[[this]]

Expression and Regulation

Operon

''[[protein|yitB]]-[[protein|yitA]]-[[protein|yisZ]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigK]] [Pubmed|15699190,15383836]

Expression and Regulation

Regulation

expressed during [SW|sporulation] in the mother cell ([[protein|SigK]]) [Pubmed|15699190,15383836]

References

15699190, 15383836

15699190, 15383836, 18991405

proteinLength

236

geneLength

711

Gene

Coordinates

1,171,755 → 1,172,465

The protein

Structure

[PDB|2OQ2] (from yeast, 33% identity) [pubmed|18991405]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A504 (yitB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/504 NBRP B. subtilis, Japan]

BKE10930 (Δ[[gene|yitB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG

BKK10930 (Δ[[gene|yitB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG, downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG