Wed Jan 20 2016 14:31:04 GMT+0100 (CET)2025-05-18 11:29:57
description
ferrochelatase, catalyzes the final step of heme b biosynthesis
coproporphyrin ferrochelatase
locus
BSU10130
BSU_10130
function
heme biosynthesis
biosynthesis of heme
product
ferrochelatase
coproporphyrin ferrochelatase
ec
4.99.1.1
outlinks
bsu
BSU10130
BSU_10130
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors]
Gene
Coordinates on the chromosome (coding sequence)
1,087,250 -> 1,088,182
The protein
Catalyzed reaction/ biological activity
insertion of Fe(2
coproporphyrin --> coproheme [pubmed|25646457]
2 H+ + heme b --> Fe2+ + protoporphyrin IX (according to UniProt)
The protein
Protein family
ferrochelatase family (according to Swiss-Prot)
ferrochelatase family (with [[protein|YlnF]], according to UniProt)
The protein
Structure
[PDB|2HK6] (complex with iron), [PDB|1AK1]
[PDB|2HK6] (complex with iron) [pubmed|17198378]
[PDB|1AK1] [pubmed|9384565]
The protein
[SW|Interactions]
([[protein|Fra]])-[[protein|HemH]], requires iron, Kd of 1.63 ± 0.02 μM [Pubmed|25826316]
Expression and Regulation
Operon
''[[protein|hemE]]-[[protein|hemH]]-[[protein|hemY]]'' [Pubmed|1459957]
References
proteinLength
310
geneLength
933
Gene
Coordinates
1,087,250 → 1,088,182
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE10130 (Δ[[gene|hemH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA, downstream forward: _UP4_GGACGTTAAAGAAGGCGATG
BKK10130 (Δ[[gene|hemH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA, downstream forward: _UP4_GGACGTTAAAGAAGGCGATG
References
Reviews
References
Original Publications