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Wed Jan 20 2016 14:31:04 GMT+0100 (CET)2025-05-18 11:29:57

description

ferrochelatase, catalyzes the final step of heme b biosynthesis

coproporphyrin ferrochelatase

locus

BSU10130

BSU_10130

function

heme biosynthesis

biosynthesis of heme

product

ferrochelatase

coproporphyrin ferrochelatase

ec

4.99.1.1

outlinks

bsu

BSU10130

BSU_10130

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors]

Gene

Coordinates on the chromosome (coding sequence)

1,087,250 -> 1,088,182

The protein

Catalyzed reaction/ biological activity

insertion of Fe(2) into protoporphyrin IX

coproporphyrin --> coproheme [pubmed|25646457]

2 H+ + heme b --> Fe2+ + protoporphyrin IX (according to UniProt)

The protein

Protein family

ferrochelatase family (according to Swiss-Prot)

ferrochelatase family (with [[protein|YlnF]], according to UniProt)

The protein

Structure

[PDB|2HK6] (complex with iron), [PDB|1AK1]

[PDB|2HK6] (complex with iron) [pubmed|17198378]

[PDB|1AK1] [pubmed|9384565]

The protein

[SW|Interactions]

([[protein|Fra]])-[[protein|HemH]], requires iron, Kd of 1.63 ± 0.02 μM [Pubmed|25826316]

Expression and Regulation

Operon

''[[protein|hemE]]-[[protein|hemH]]-[[protein|hemY]]'' [Pubmed|1459957]

References

9384565, 1459957, 8119288, 21052751, 23097001, 16453119, 25826316

proteinLength

310

geneLength

933

Gene

Coordinates

1,087,250 → 1,088,182

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE10130 (Δ[[gene|hemH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA, downstream forward: _UP4_GGACGTTAAAGAAGGCGATG

BKK10130 (Δ[[gene|hemH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTTTACACCCTCTTTA, downstream forward: _UP4_GGACGTTAAAGAAGGCGATG

References

Reviews

28123057

References

Original Publications

9384565, 1459957, 8119288, 21052751, 23097001, 16453119, 25826316, 28864672, 17198378, 9384565, 12761666, 10704318, 25646457