2018-02-07 12:27:522025-04-05 10:38:46
description
primary bacitracin resistance determinant, ABC transporter (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system
primary bacitracin resistance determinant, [SW|ABC transporter] (permease), sensory component of the Bce regulatory & detoxification system, protects cell wall biosynthetic targets from inhibition by antimicrobial peptides
locus
BSU30370
BSU_30370
geneLength
1938
1941
function
export of toxic peptides
protection of cell wall biosynthetic targets from inhibition by antimicrobial peptides
product
bacitracin ABC transporter (permease)
[SW|ABC transporter] for target protection of cell wall synthesis
ec
outlinks
bsu
BSU30370
BSU_30370
The protein
Catalyzed reaction/ biological activity
binds bacitracin, KD: 60 nM [Pubmed|25118291]
inhibits [[protein|BceS]] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]
binds bacitracin, KD: 60 nM [Pubmed|25118291]
inhibits [[protein|BceS]] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]
releaves intermediates of the lipid II cycle from the inhibitory interaction with antimicrobial peptides such as bacitracin [pubmed|31871088]
The protein
Protein family
ABC-4 integral membrane protein family (according to Swiss-Prot)
[SW|ABC-4 integral membrane protein family] (according to UniProt)
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (according to UniProt)
Biological materials
Mutant
MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
BKE30370 (Δ[[gene|bceB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
BKK30370 (Δ[[gene|bceB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
GP3218 Δ([[gene|bceR]]-[[gene|bceS]]-[[gene|bceA]]-[[gene|bceB]])::''cat'', available in [SW|Jörg Stülke]'s lab
MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
BKE30370 (Δ[[gene|bceB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
BKK30370 (Δ[[gene|bceB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
References
Reviews
References
Original publications
The protein
Paralogous protein(s)
[[this]]
labs
[SW|Susanne Gebhard], Bath, UK