2017-10-19 11:53:582025-05-27 12:25:53
description
flagellin protein, about 20,000 subunits make up one flagellum
flagellin protein, about 12,000 subunits make up one flagellum
locus
BSU35360
BSU_35360
geneLength
912
915
function
motility and chemotaxis
[SW|motility and chemotaxis]
outlinks
bsu
BSU35360
BSU_35360
Gene
Phenotypes of a mutant
no swarming motility on B medium. [Pubmed|19202088]
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
few hours delay in pellicle development [Pubmed|26122431]
mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain [Pubmed|26122431]
increased [[protein|DegU]]-P formation, resulting in reduced [[gene|comK]] expression and strongly reduced [SW|genetic competence] [pubmed|28800172]
no swarming motility on B medium [Pubmed|19202088]
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
few hours delay in pellicle development [Pubmed|26122431]
mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain [Pubmed|26122431]
a substitution of Ala233 to Val results in straight flagella [pubmed|28800172]
The protein
Protein family
bacterial flagellin family (according to Swiss-Prot)
bacterial flagellin family (with [[protein|FlgL]] and [[protein|YvzB]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YvzB]] (C-terminal domain of Hag)
[[this]]
The protein
Structure
[PDB|3A5X] (from ''Salmonella typhimurium'', 42% identity)
the structure of flagellar filaments: [pubmed|29038601]
[PDB|3A5X] (from ''Salmonella typhimurium'', 42% identity) [pubmed|20228803]
the structure of flagellar filaments: [pubmed|29038601]
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
12.000 monomers make up one flagellum [pubmed|31113895]
Biological materials
Expression vector
expression in ''B. subtilis'', in [SW|pBQ200]: pGP1089, available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP1035 (in [[protein|pAC6]]), pGP755 (in [[protein|pAC7]]), there is also a series of promoter deletion variants in [[protein|pAC6]] and [[protein|pAC7]] [Pubmed|21856853], all available in [SW|Jörg Stülke]'s lab
pGP1035 (in [SW|pAC6]), pGP755 (in [SW|pAC7]), there is also a series of promoter deletion variants in [SW|pAC6] and [SW|pAC7] [Pubmed|21856853], all available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Daniel Kearns]
References
Original Publications
Biological materials
Expression vectors
expression in ''B. subtilis'', in [SW|pBQ200]: pGP1089, available in [SW|Jörg Stülke]'s lab
labs
[SW|Daniel Kearns]