2019-01-30 09:54:192018-05-20 18:39:18

geneLength

771

768

The protein

Structure

[PDB|4EQM] (PknB from Staphylococcus aureus, 34% identity in the N-terminal part, aa 18 ... 174) [pubmed|22701750]

[PDB|4EQM] (PknB from Staphylococcus aureus, 34% identity in the N-terminal part, aa 18 ... 174)

Biological materials

Expression vectors

pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Stülke] lab, [pubmed|20389117]

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Stülke] lab

References

20389117, 12406230, 21304896, 24731262, 25278935, 22701750

20389117, 12406230, 21304896, 24731262, 25278935

Biological materials

Expression vector

pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Stülke] lab, [pubmed|20389117]

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Stülke] lab