Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-20 23:26:21
description
putative cysteine ABC transporter (binding protein), the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
putative S-(2-succino)cysteine [SW|ABC transporter] (binding protein)
locus
BSU39500
BSU_39500
function
uptake of cysteine
uptake and utilization of S-(2-succino)cysteine
product
putative cysteine ABC transporter (binding protein)
putative S-(2-succino)cysteine [SW|ABC transporter] (binding protein)
ec
outlinks
bsu
BSU39500
BSU_39500
aminos
MKMKKWTVLVVAALLAVLSACGNGNSSSKEDDNVLHVGATGQSYPFAYKENGKLTGFDVEVMEAVAKKIDMKLDWKLLEFSGLMGELQTGKLDTISNQVAVTDERKETYNFTKPYAYAGTQIVVKKDNTDIKSVDDLKGKTVAAVLGSNHAKNLESKDPDKKINIKTYETQEGTLKDVAYGRVDAYVNSRTVLIAQIKKTGLPLKLAGDPIVYEQVAFPFAKDDAHDKLRKKVNKALDELRKDGTLKKLSEKYFNEDITVEQKH
[SW|Categories] containing this gene/protein
[SW|ABC transporters], [SW|biosynthesis/ acquisition of amino acids], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
4,059,488 -> 4,060,282
The protein
Protein family
bacterial solute-binding protein 3 family (according to Swiss-Prot)
[SW|bacterial solute-binding protein 3 family] (according to UniProt)
The protein
[SW|Interactions]
[[protein|YxeO]]-[[protein|YxeN]]-[[protein|YxeM]] [Pubmed|10092453]
Expression and Regulation
Operon
''[[protein|yxeK]]-[[protein|snaB]]-[[protein|yxeM]]-[[protein|yxeN]]-[[protein|yxeO]]-[[protein|sndB]]-[[protein|yxeQ]]'' [Pubmed|10746760]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|16513748]
Expression and Regulation
Regulation
repressed in the presence of cysteine ([[protein|CymR]]) [Pubmed|16513748]
Expression and Regulation
Regulatory mechanism
[[protein|CymR]]: transcription repression [Pubmed|16513748]
Expression and Regulation
Additional information
the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] [PubMed|21815947]
Biological materials
Mutant
1A950 ( ''yxeM''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
MGNA-B782 (yxeM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1781 NBRP B. subtilis, Japan]
1A950 ( ''yxeM''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
BKE39500 (Δ[[gene|yxeM]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT, downstream forward: _UP4_TAAGAAACGGCGGTGACTCA
BKK39500 (Δ[[gene|yxeM]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT, downstream forward: _UP4_TAAGAAACGGCGGTGACTCA
References
proteinLength
264
geneLength
795
Gene
Coordinates
4,059,488 → 4,060,282
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|4ZV1] (synthetic, 38% identity) [pubmed|26853627]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]