SubtiBank SubtiBank
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Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-12 06:37:25

description

K /H antiporter for K efflux

K+/H+ antiporter for K+ efflux

locus

BSU09860

BSU_09860

product

K /H antiporter for K efflux

K+/H+ antiporter for K+ efflux

ec

outlinks

bsu

BSU09860

BSU_09860

aminos

MNIKENDLPGIGKKFEIETRSHEKMTIIIHDDGRREIYRFNDRDPDELLSNISLDDSEARQIAAILGGMVYKPQALESIEMAFSDLIIEWFKVEKGAKSIGRTLGELDVRQNYDVTVIAIIKHNQEKLLNPGADSIIEENDTLVLSGERKHLKKLIHDFLSGEGV

[SW|Categories] containing this gene/protein

[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

1,060,427 -> 1,060,924

The protein

[SW|Domains]

contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:RCKC-terminaldomain* x])

contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot)

cell membrane (peripheral) (according to UniProt)

The protein

[SW|Interactions]

[[protein|KhtT]]-[[protein|KhtU]] [Pubmed|17679694]

Expression and Regulation

Operon

''[[protein|khtS]]-[[protein|khtT]]-[[protein|khtU]]'' [Pubmed|22383849]

Biological materials

Mutant

GP2078 (''[[protein|khtS]]-[[protein|khtT]]-[[protein|khtU]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]

GP2078 (Δ[[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]::''aphA3''), available in [SW|Jörg Stülke]'s lab

BKE09860 (Δ[[gene|khtT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

BKK09860 (Δ[[gene|khtT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

Biological materials

Expression vector

pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

14987767, 17679694, 24330391

proteinLength

165

geneLength

498

Gene

Coordinates

1,060,427 → 1,060,924

The protein

Paralogous protein(s)

[[this]]

The protein

Effectors of protein activity

binds c-di-AMP, which likely activates export activity [pubmed|31061098]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

Reviews

27935846, 31361596, 32603625

References

Original publications

14987767, 17679694, 24330391, 31061098