Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-12 06:37:25
description
K /H antiporter for K efflux
K+/H+ antiporter for K+ efflux
locus
BSU09860
BSU_09860
product
K /H antiporter for K efflux
K+/H+ antiporter for K+ efflux
ec
outlinks
bsu
BSU09860
BSU_09860
aminos
MNIKENDLPGIGKKFEIETRSHEKMTIIIHDDGRREIYRFNDRDPDELLSNISLDDSEARQIAAILGGMVYKPQALESIEMAFSDLIIEWFKVEKGAKSIGRTLGELDVRQNYDVTVIAIIKHNQEKLLNPGADSIIEENDTLVLSGERKHLKKLIHDFLSGEGV
[SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
1,060,427 -> 1,060,924
The protein
[SW|Domains]
contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:
contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane (peripheral) (according to UniProt)
The protein
[SW|Interactions]
[[protein|KhtT]]-[[protein|KhtU]] [Pubmed|17679694]
Expression and Regulation
Operon
''[[protein|khtS]]-[[protein|khtT]]-[[protein|khtU]]'' [Pubmed|22383849]
Biological materials
Mutant
GP2078 (''[[protein|khtS]]-[[protein|khtT]]-[[protein|khtU]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]
GP2078 (Δ[[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE09860 (Δ[[gene|khtT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
BKK09860 (Δ[[gene|khtT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
Biological materials
Expression vector
pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
proteinLength
165
geneLength
498
Gene
Coordinates
1,060,427 → 1,060,924
The protein
Paralogous protein(s)
[[this]]
The protein
Effectors of protein activity
binds c-di-AMP, which likely activates export activity [pubmed|31061098]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original publications