2017-8-11 16:0:32025-05-24 02:18:53
locus
BSU25710
BSU_25710
geneLength
750
753
outlinks
bsu
BSU25710
BSU_25710
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
The protein
Protein family
N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
[SW|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|CwlA]], [[protein|XlyA]], [[protein|XlyB]]
[[this]]
The protein
[SW|Domains]
contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|XlyA]], [[protein|XlyB]])
contains an amidase_2 domain (like [[protein|BlyA]], [[protein|CwlA]], [[protein|XlyA]], [[protein|XlyB]])
[SW|N-acetylmuramoyl-L-alanine amidase domain] (aa 45-141) (according to UniProt)
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
secreted (according to UniProt)
References
The protein
Structure
[PDB|3RDR] (N-terminal domain, aa 1 - 150, XlyA, 75% identity) [pubmed|21816821]
[PDB|1LBU] (C-terminal peptidoglycan-binding domain, aa 183 - 245, 48% identity)
Biological materials
Mutant
BKE25710 (Δ[[gene|cwlH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA
BKK25710 (Δ[[gene|cwlH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATCCCTCCCTATT, downstream forward: _UP4_TAAATGCAGGATTTAACGGA