2017-10-31 11:41:462025-02-19 15:06:19
description
two-component response regulator, regulation of degradative enzyme expression, genetic competence, biofilm formation, capsule biosynthesis (together with SwrAA), non-phosphorylated DegU is required for swarming motility
two-component response regulator, regulation of degradative enzyme expression, [SW|genetic competence], [SW|biofilm formation], capsule biosynthesis (together with [[protein|SwrA]]), non-phosphorylated DegU is required for swarming motility
locus
BSU35490
BSU_35490
geneLength
687
690
function
regulation of degradative enzymes, genetic competence,
regulation of degradative enzymes, [SW|genetic competence], and other adaptive responses
outlinks
bsu
BSU35490
BSU_35490
The protein
Modification
phosphorylated by [[protein|DegS]] on Asp-56 [Pubmed|1901568], this modulates DNA-binding activity
phosphorylation by [[protein|DegS]] occurs in response to inhibition of flagellar rotation [Pubmed|23888912]
phosphorylated by [[protein|DegS]] on Asp-56 [Pubmed|1901568], this modulates DNA-binding activity
phosphorylation by [[protein|DegS]] occurs in response to inhibition of flagellar rotation [Pubmed|28800172,23888912]
The protein
Additional information
[[protein|DegU]]-P is degraded by [[protein|ClpC]]-[[protein|ClpP]] [Pubmed|20070525]
accumulation of [[protein|DegU]]-P results in decreased transcription of the [[gene|epsA]]-[[gene|epsO]] and [[gene|tapA]]-[[gene|sipW]]-[[gene|tasA]] operons due to increased levels of [[protein|Spo0A]]-P [Pubmed|24123822]
[[protein|DegU]]-P is degraded by [[protein|ClpC]]-[[protein|ClpP]] [Pubmed|20070525]
accumulation of [[protein|DegU]]-P results in decreased transcription of the [[gene|epsA]]-[[gene|epsO]] and [[gene|tapA]]-[[gene|sipW]]-[[gene|tasA]] operons due to increased levels of [[protein|Spo0A]]-P [Pubmed|24123822]
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000030 PRODORIC2 database]
Biological materials
Mutant
BKE35490 (Δ[[gene|degU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
BKK35490 (Δ[[gene|degU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
GP2644([[gene|degU]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE35490 (Δ[[gene|degU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
BKK35490 (Δ[[gene|degU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT, downstream forward: _UP4_TAGTATAATAGGAGACTTGC
References
Original Publications
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Response regulatory domain] (aa 5-121) (according to UniProt)
[SW|HTH luxR-type domain] (aa 159-224) (according to UniProt)