SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:56:02 GMT+0200 (CEST)2025-05-11 17:49:05

locus

BSU27870

BSU_27870

outlinks

bsu

BSU27870

BSU_27870

syns

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors], [SW|phosphoproteins]

The protein

Catalyzed reaction/ biological activity

L-aspartate + O2 = iminosuccinate + H2O2 (according to Swiss-Prot)

L-aspartate + O2 --> H2O2 + iminosuccinate (according to UniProt)

The protein

Protein family

NadB subfamily (according to Swiss-Prot)

FAD-dependent oxidoreductase 2 family (with [[protein|SdhA]], according to UniProt)

Expression and Regulation

Operon

''[[protein|nadB]]-[[protein|nadC]]-[[protein|nadA]]'' [Pubmed|8444804]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|8444804]

Expression and Regulation

Regulation

repressed in the presence of nicotinic acid ([[protein|NadR]]) [Pubmed|16199587]

Expression and Regulation

Regulatory mechanism

[[protein|NadR]]: transcription repression [Pubmed|16199587]

Labs working on this gene/protein

[SW|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]

proteinLength

531

geneLength

1596

Gene

Coordinates

2,847,871 → 2,849,466

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

FAD (according to UniProt)

The protein

Structure

[PDB|5KXJ] (from Salmonella typhimurium, 38% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE27870 (Δ[[gene|nadB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTG

BKK27870 (Δ[[gene|nadB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGCCATCCTCCTGTTG, downstream forward: _UP4_AGAAAAAACGAGGGGATTTG

labs

[SW|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]