SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-25 04:47:59

locus

BSU08000

BSU_08000

ec

outlinks

bsu

BSU08000

BSU_08000

aminos

MINITAITTEHQLLKNIPIERVEQPDIAWYWVDFYGPEDTETALLRDFFHFHPLAIEDCFQHMQRPKLDHYDGYRFYVIHALNKETLETEEVDIFQGEKFVVTFHLHETPGIAKVRERLYASPDILKKGPGHISYMIMDQLVDEYFPLVYKIEDRLNEIEESRPHKTYGTLMNEVFDLRTDLLHLRRTIIPMRDLLYRILSLDHVKEQRETKAYFSDIYDHLLKLSEIVESNRDMTSDLRDSYVTLNSNRMNAIMMTLTIVSTIFIPLTFIAGVYGMNFDFMPELHWKYGYFAVLGLMAALVIGMLIWFVHKGWFNIFK

[SW|Categories] containing this gene/protein

[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

871,347 -> 872,306

Gene

Phenotypes of a mutant

a ''[[protein|mgtE]] [[protein|yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722]

a ''[[gene|mgtE]] [[gene|yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [[protein|CitM]]) [Pubmed|24415722]

Expression and Regulation

Operon

''yfjQ

References

24415722

24415722, 23425532

proteinLength

319

geneLength

960

Gene

Coordinates

871,347 → 872,306

The protein

Protein family

CorA metal ion transporter (MIT) (TC 1.A.35) family (together with [[protein|CorA]]) (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|4I0U] (from Thermotoga maritima, 37% identity) [pubmed|23425532]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]

BKE08000 (Δ[[gene|yfjQ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT

BKK08000 (Δ[[gene|yfjQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT

GP2853 (''[[gene|yfjQ]]''::''tet''), available in [SW|Jörg Stülke]'s lab