Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-11 06:59:25
locus
BSU29010
BSU_29010
outlinks
subtilist
gene_detail+BG13832
gene_detail BG13832
outlinks
bsu
BSU29010
BSU_29010
aminos
METMGRHVISELWGCDFDKLNDMDFIEKTFVNAALKSGAEVREVAFHKFAPQGVSGVVIISESHLTIHSFPEHGYASIDVYTCGDLDPNVAADYIAEALHADTRENIEIPRGMGPVQIKQAQAKVL
[SW|Categories] containing this gene/protein
[SW|miscellaneous metabolic pathways], [SW|biofilm formation]
Gene
Coordinates on the chromosome (coding sequence)
2,966,413 -> 2,966,793
The protein
Catalyzed reaction/ biological activity
S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt + CO2 (according to Swiss-Prot)
H+ + S-adenosyl-L-methionine --> CO2 + S-adenosyl 3-(methylsulfanyl)propylamine (according to UniProt)
The protein
Protein family
Type 1 subfamily (according to Swiss-Prot)
prokaryotic AdoMetDC family (single member, according to UniProt)
Expression and Regulation
Operon
''[[protein|gapB]]-[[protein|speD]]'' [Pubmed|15720552]
''[[protein|speD]]'' [Pubmed|10844697]
Expression and Regulation
[SW|Sigma factor]
''[[protein|gapB]]'': [[protein|SigA]] [Pubmed|15720552]
''[[protein|speD]]'': [[protein|SigA]] [Pubmed|10844697]
Expression and Regulation
Regulation
''[[protein|gapB]]'': repressed in the presence of glucose ([[protein|CcpN]]) [Pubmed|15720552]
Expression and Regulation
Regulatory mechanism
[[protein|CcpN]]: transcription repression [Pubmed|15720552]
Expression and Regulation
Additional information
'[[protein|speD]]': the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) [PubMed|21815947]
References
proteinLength
126
geneLength
381
Gene
Coordinates
2,966,413 → 2,966,793
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A122 (ytcF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/122 NBRP B. subtilis, Japan]
BKE29010 (Δ[[gene|speD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACA
BKK29010 (Δ[[gene|speD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTCATGGACCCCCCTT, downstream forward: _UP4_TAAAGTAAATTGCGAACACA
GP2599 (Δ[[gene|speD]]::tet comIQ12L) (in DK1042), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
pGP2324: for expression in B. subtilis, in [SW|pBQ200],available in [SW|Jörg Stülke]'s lab