SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-01-20 14:33:31

locus

BSU06460

BSU_06460

outlinks

bsu

BSU06460

BSU_06460

aminos

MYKVKVYVSLKESVLDPQGSAVQHALHSMTYNEVQDVRIGKYMELTIEKSDRDLDVLVKEMCEKLLANTVIEDYRYEVEEVVAQ

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]

Gene

Coordinates on the chromosome (coding sequence)

702,319 -> 702,573

The protein

Protein family

UPF0062 family (according to Swiss-Prot)

UPF0062 family (single member, according to UniProt)

The protein

[SW|Interactions]

[[protein|PurS]]-[[protein|PurS]] [Pubmed|15301530]

[[protein|PurS]]-[[protein|PurQ]]-[[protein|PurL]] [Pubmed|15301530]

Expression and Regulation

Operon

''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|3036807]

Expression and Regulation

Regulation

repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]

repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]

repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]

Expression and Regulation

Regulatory mechanism

[PurR]]: transcription repression [Pubmed|7638212]

[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]

[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]

Expression and Regulation

Additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

proteinLength

84

geneLength

255

Gene

Coordinates

702,319 → 702,573

The protein

Catalyzed reaction/ biological activity

ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A920 (yexA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/920 NBRP B. subtilis, Japan]

BKE06460 (Δ[[gene|purS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC

BKK06460 (Δ[[gene|purS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC