Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-01-20 14:33:31
locus
BSU06460
BSU_06460
outlinks
bsu
BSU06460
BSU_06460
aminos
MYKVKVYVSLKESVLDPQGSAVQHALHSMTYNEVQDVRIGKYMELTIEKSDRDLDVLVKEMCEKLLANTVIEDYRYEVEEVVAQ
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
702,319 -> 702,573
The protein
Protein family
UPF0062 family (according to Swiss-Prot)
UPF0062 family (single member, according to UniProt)
The protein
[SW|Interactions]
[[protein|PurS]]-[[protein|PurS]] [Pubmed|15301530]
[[protein|PurS]]-[[protein|PurQ]]-[[protein|PurL]] [Pubmed|15301530]
Expression and Regulation
Operon
''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|3036807]
Expression and Regulation
Regulation
repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]
repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]
repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]
Expression and Regulation
Regulatory mechanism
[PurR]]: transcription repression [Pubmed|7638212]
[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]
Expression and Regulation
Additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
proteinLength
84
geneLength
255
Gene
Coordinates
702,319 → 702,573
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A920 (yexA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/920 NBRP B. subtilis, Japan]
BKE06460 (Δ[[gene|purS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC
BKK06460 (Δ[[gene|purS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC