SubtiBank SubtiBank
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Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-09 06:39:49

description

transcriptional regulator (LysR family), activator of the ywbH-ywbG operon

transcriptional regulator ([SW|LysR family]), activator of the [[gene|ywbH]]-[[gene|ywbG]] operon

locus

BSU38310

BSU_38310

function

control of expression of the ywbH-ywbG operon

control of expression of the [[gene|ywbH]]-[[gene|ywbG]] operon

product

transcriptional regulator (LysR family)

transcriptional regulator ([SW|LysR family])

ec

outlinks

bsu

BSU38310

BSU_38310

aminos

MDIRHLTYFLEVARLKSFTKASQSLYVSQPTISKMIKNLEEELGIELFYRNGRQVELTDAGHSMYVQAQEIIKSFQNLTSELNDIMEVKKGHVRIGLPPMIGSGFFPRVLGDFRENYPNVTFQLVEDGSIKVQEGVGDGSLDIGVVVLPANEDIFHSFTIVKETLMLVVHPSHRLADEKECQLRELKDEPFIFFREDFVLHNRIMTECIKAGFRPHIIYETSQWDFISEMVSANLGIGLLPERICRGLDPEKVKVIPLVDPVIPWHLAIIWRKDRYLSFAARAWLEHTKSYLWDPKKDSKG

[SW|Categories] containing this gene/protein

[SW|transcription factors and their control]

Gene

Coordinates on the chromosome (coding sequence)

3,932,198 -> 3,933,103

The protein

Protein family

[SW|LysR family]

[SW|LysR family] (according to UniProt)

Expression and Regulation

Operon

''[[protein|ywbI]]-[[protein|thiM]]-[[protein|thiE]]'' [Pubmed|9139923]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|9139923]

proteinLength

301

geneLength

906

Gene

Coordinates

3,932,198 → 3,933,103

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH lysR-type domain] (aa 1-58) (according to UniProt)

The protein

Structure

[PDB|3FZV] (from Pseudomonas aeruginosa, 28% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B675 (ywbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1674 NBRP B. subtilis, Japan]

BKE38310 (Δ[[gene|ywbI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA, downstream forward: _UP4_AAAGATAGTAAAGGATGATG

BKK38310 (Δ[[gene|ywbI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA, downstream forward: _UP4_AAAGATAGTAAAGGATGATG