2017-05-17 10:08:062025-05-17 08:16:56
description
sulphur compound N-deacetylase, the yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ operon may be implicated in the utilization of a modified cysteine variant
N-acetylcysteine deacetylase
locus
BSU39470
BSU_39470
geneLength
1140
1143
function
unknown
utilization and detoxification of S-(2-succino)cysteine
product
sulphur compound N-deacetylase
N-acetylcysteine deacetylase
outlinks
bsu
BSU39470
BSU_39470
Gene
Phenotypes of a mutant
a ''[[gene|sndA]] [[gene|sndB]] [[gene|sndC]]'' triple mutant does not grow with N-acetyl cysteine [Pubmed|23944997]
a ''[[gene|sndA]] [[gene|sndB]] [[gene|sndC]]'' triple mutant does not grow with N-acetyl cysteine and S-(2-succino)cysteine as the single sources of sulfur [Pubmed|23944997,29626092]
The protein
Protein family
peptidase M20 family (according to Swiss-Prot)
[SW|peptidase M20 family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|SndA]], [[protein|SndC]], [[protein|YkuR]],[[protein|AmhX]]
[[this]]
Biological materials
Mutant
MGNA-B712 (yxeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1711 NBRP B. subtilis, Japan]
MGNA-B712 (yxeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1711 NBRP B. subtilis, Japan]
BKE39470 (Δ[[gene|sndB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC, downstream forward: _UP4_GTTATTGTATTGGAGACCAT
BKK39470 (Δ[[gene|sndB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC, downstream forward: _UP4_GTTATTGTATTGGAGACCAT
References
The protein
Catalyzed reaction/ biological activity
N-acetylcysteine + oxaloacetate --> cysteine + acetate [Pubmed|29626092]