SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-03-13 17:56:25

locus

BSU29100

BSU_29100

geneLength

1737

1740

ec

outlinks

bsu

BSU29100

BSU_29100

The protein

Catalyzed reaction/ biological activity

autophosphorylation, phosphorylation of [[protein|PhoP]]

autophosphorylation, phosphorylation of [[protein|PhoP]]

ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

The protein

[SW|Domains]

two transmembrane segments

[SW|PAS domain], for binding of an intermediate of wall teichoic acid biosynthesis [Pubmed|25315493]

C-terminal histidine phosphotransferase domain

two transmembrane segments

[SW|PAS domain], for binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate)) [Pubmed|25315493]

[SW|Histidine kinase domain] (aa 357-575) (according to UniProt)

The protein

Effectors of protein activity

activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis to the intracellular [SW|PAS domain] of [[protein|PhoR]] [Pubmed|25315493]

activity is inhibited by binding of an intermediate of wall teichoic acid biosynthesis (lipid V composed of poly(glycerol phosphate) to the intracellular [SW|PAS domain] of [[protein|PhoR]] [Pubmed|25315493]

the kinase activity is activated by an intermediate in the WTA biosynthetic pathway (product of [[protein|TagA]] or [[protein|TagB]]) [pubmed|29644746]

Biological materials

Mutant

1A966 ( ''phoR''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]

1A966 ( ''phoR''::''tet''), [Pubmed|14973033], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A966&Search=1A966 BGSC]

BKE29100 (Δ[[gene|phoR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA

BKK29100 (Δ[[gene|phoR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGAAAAAAGGCGCACAC, downstream forward: _UP4_TAATGTTTACAAAGGTTTAA

Labs working on this gene/protein

[SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

Reviews

10094672, 25355628

10094672, 25355628, 30459743

References

Original publications

20008068, 10433720, 15205429, 9084179, 17085571, 9987123, 10913081, 16452408, 20167622, 15205429, 14762014, 20382764, 25315493

20008068, 10433720, 15205429, 9084179, 17085571, 9987123, 10913081, 16452408, 20167622, 15205429, 14762014, 20382764, 25315493, 29644746

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Localization]

cell membrane (according to UniProt)

labs

[SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]