_ec

locus

BSU03890

BSU_03890

geneLength

1437

1440

outlinks

bsu

BSU03890

BSU_03890

The protein

Protein family

[SW|MocR/ GabR family] [Pubmed|22020104]

[SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

The protein

Paralogous protein(s)

none, but there are 7 members of the [SW|MocR/ GabR family] in B. subtilis

[[this]]

The protein

[SW|Domains]

N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]

C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574]

N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]

C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574]

[SW|HTH gntR-type domain] (aa 14-82) (according to UniProt)

The protein

[SW|Cofactors]

gamma-aminobutyric acid, PLP [Pubmed|24127574]

gamma-aminobutyric acid, PLP [Pubmed|31848410,24127574]

The protein

Effectors of protein activity

activation of ''[[gene|gabT]]-[[gene|gabD]]'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate [Pubmed|15223311]

in the absence of GABA, GabR represses [[gene|gabT]]-[[gene|gabD]] expression, whereas binding of GABA to GabR results in a conformational change resulting in activation of transcription [pubmed|32147931]

Biological materials

Mutant

MGNA-C014 (ycnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2012 NBRP B. subtilis, Japan]

BKE03890 (Δ[[gene|gabR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTAGTTTCTCCTTC, downstream forward: _UP4_AAAATCCCCGTTACAGGGGA

BKK03890 (Δ[[gene|gabR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTAGTTTCTCCTTC, downstream forward: _UP4_AAAATCCCCGTTACAGGGGA

Labs working on this gene/protein

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

References

11756427, 15223311, 12123465, 24127574, 22020104, 25388514, 25911692, 27640111, 26681693, 28348215, 28412355

11756427, 15223311, 12123465, 24127574, 22020104, 25388514, 25911692, 27640111, 26681693, 28348215, 28412355, 29253008, 31848410, 32147931, 32413777

labs

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]