SubtiBank SubtiBank
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2020-09-09 14:32:292025-05-12 06:05:10

description

ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer

function

plasmid rolling-circle replication, conjugative transfer of ICEBs1

coupling of DNA replication, transcription, recombination and segregation

Gene

Phenotypes of a mutant

essential [Pubmed|12682299]

essential [Pubmed|12682299]

inactivation can be suppressed by inactivation of either [[gene|recA]], [[gene|recJ]], [[gene|recO]], or [[gene|mfd]] [pubmed|32793628]

The protein

Catalyzed reaction/ biological activity

PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]

dismantles [[protein|RecA]] filaments [Pubmed|20723756]

ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]

PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]

dismantles [[protein|RecA]] filaments [Pubmed|20723756]

ATP + H2O --> ADP + H+ + phosphate (according to UniProt)