2017-02-27 15:02:042025-05-13 23:39:33
description
ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer
locus
BSU06610
BSU_06610
geneLength
2217
2220
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
coupling of DNA replication, transcription, recombination and segregation
ec
outlinks
bsu
BSU06610
BSU_06610
Gene
Coordinates on the chromosome (coding sequence)
719,370 -> 721,589
Gene
Phenotypes of a mutant
essential [Pubmed|12682299]
essential [Pubmed|12682299]
inactivation can be suppressed by inactivation of either [[gene|recA]], [[gene|recJ]], [[gene|recO]], or [[gene|mfd]] [pubmed|32793628]
The protein
Catalyzed reaction/ biological activity
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
dismantles [[protein|RecA]] filaments [Pubmed|20723756]
coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
dismantles [[protein|RecA]] filaments [Pubmed|20723756]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
The protein
Protein family
uvrD-like helicase C-terminal domain (according to Swiss-Prot)
[SW|helicase family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|YjcD]]
[[this]]
The protein
[SW|Domains]
two RecA-like domains [Pubmed|24147116]
intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
two RecA-like domains [Pubmed|24147116]
intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
[SW|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)
[SW|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)
The protein
Structure
[PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
[PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
[PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
[PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
[PDB|5DMA], C-terminal [SW|RNA polymerase] interaction domain [pubmed|28160601]
Expression and Regulation
[[this]]
References
Reviews
References
Original Publications
Gene
Coordinates
719,370 → 721,589
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]