SubtiBank SubtiBank
Version comparison:

2017-05-17 11:12:482025-05-23 10:45:59

locus

BSU32050

BSU_32050

geneLength

1500

1503

ec

outlinks

bsu

BSU32050

BSU_32050

The protein

Catalyzed reaction/ biological activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro (according to Swiss-Prot)

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low (according to UniProt)

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids (according to UniProt)

The protein

Protein family

peptidase M17 family (according to Swiss-Prot)

peptidase M17 family (single member, according to UniProt)

Biological materials

Mutant

MGNA-B569 (yuiE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1568 NBRP B. subtilis, Japan]

MGNA-B569 (yuiE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1568 NBRP B. subtilis, Japan]

BKE32050 (Δ[[gene|yuiE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGCTTACACTCCTAA, downstream forward: _UP4_TAATGAAAAATCCCTGCTGT

BKK32050 (Δ[[gene|yuiE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGGCTTACACTCCTAA, downstream forward: _UP4_TAATGAAAAATCCCTGCTGT

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|3H8E] (from Pseudomonas putida 37% identity) [pubmed|20359484]

References

Research papers

20359484, 32949815