SubtiBank SubtiBank
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Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-31 02:44:00

title

msmE

melE

description

probably ABC transporter for melibiose (binding protein)

subunit of [SW|ABC transporter] for melibiose and raffinose (binding lipoprotein)

locus

BSU30270

BSU_30270

function

probably melibiose uptake

uptake of melibiose and raffinose

product

ABC transporter (binding protein)

[SW|ABC transporter] (binding protein)

ec

names

msmE

melE

msmE

outlinks

bsu

BSU30270

BSU_30270

aminos

MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHFPGNDYENMMRVRMAANDLPDLFDTHGWGKIRYGEYTADLRDMKWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIAPPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQWSKFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQINPNVKVGIIPTPAIHPGDDPIWIGGERYTLAAWKDSPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKADIFYANDYEYYQDVKVEPYFDRLYLPNGMWDVLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE

[SW|Categories] containing this gene/protein

[SW|ABC transporters], [SW|utilization of specific carbon sources], [SW|membrane proteins], [SW|sporulation proteins]

Gene

Coordinates on the chromosome (coding sequence)

3,097,850 -> 3,099,130

The protein

[SW|Localization]

extracellular (signal peptide), associated to the cell membrane (via [[protein|AmyC]]-[[protein|AmyD]]) [Pubmed|18957862,10092453]

extracellular (signal peptide), associated to the cell membrane (via [[protein|MelC]]-[[protein|MelD]]) [Pubmed|18957862,10092453]

The protein

[SW|Interactions]

([[protein|AmyC]]-[[protein|AmyD]])-[[protein|MsmE]] [Pubmed|10092453]

Expression and Regulation

Operon

''[[protein|msmR]]-[[protein|msmE]]-[[protein|amyD]]-[[protein|amyC]]-[[protein|melA]]'' [Pubmed|22383849]

''[[protein|msmE]]-[[protein|amyD]]-[[protein|amyC]]-[[protein|melA]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

''[[protein|msmR]]'': [[protein|SigA]] [Pubmed|22900538]

Expression and Regulation

Regulation

[[protein|MsmR]]

repressed by glucose ([[protein|CcpA]]) [Pubmed|22900538]

Expression and Regulation

Regulatory mechanism

[[protein|CcpA]]: transcription repression [Pubmed|22900538]

References

10092453, 22383849, 22900538, 18957862

10092453, 22383849, 22900538, 18957862, 31138628

proteinLength

426

geneLength

1281

Gene

Coordinates

3,097,850 → 3,099,130

The protein

Catalyzed reaction/ biological activity

binding of melibiose and raffinose [pubmed|31138628]

The protein

Protein family

[SW|Bacterial solute-binding protein 1 family] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A801 (msmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/801 NBRP B. subtilis, Japan]

BKE30270 (Δ[[gene|melE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA

BKK30270 (Δ[[gene|melE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA