Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-31 02:44:00
title
msmE
melE
description
probably ABC transporter for melibiose (binding protein)
subunit of [SW|ABC transporter] for melibiose and raffinose (binding lipoprotein)
locus
BSU30270
BSU_30270
function
probably melibiose uptake
uptake of melibiose and raffinose
product
ABC transporter (binding protein)
[SW|ABC transporter] (binding protein)
ec
names
msmE
melE
msmE
outlinks
bsu
BSU30270
BSU_30270
aminos
MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHFPGNDYENMMRVRMAANDLPDLFDTHGWGKIRYGEYTADLRDMKWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIAPPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQWSKFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQINPNVKVGIIPTPAIHPGDDPIWIGGERYTLAAWKDSPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKADIFYANDYEYYQDVKVEPYFDRLYLPNGMWDVLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE
[SW|Categories] containing this gene/protein
[SW|ABC transporters], [SW|utilization of specific carbon sources], [SW|membrane proteins], [SW|sporulation proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,097,850 -> 3,099,130
The protein
[SW|Localization]
extracellular (signal peptide), associated to the cell membrane (via [[protein|AmyC]]-[[protein|AmyD]]) [Pubmed|18957862,10092453]
extracellular (signal peptide), associated to the cell membrane (via [[protein|MelC]]-[[protein|MelD]]) [Pubmed|18957862,10092453]
The protein
[SW|Interactions]
([[protein|AmyC]]-[[protein|AmyD]])-[[protein|MsmE]] [Pubmed|10092453]
Expression and Regulation
Operon
''[[protein|msmR]]-[[protein|msmE]]-[[protein|amyD]]-[[protein|amyC]]-[[protein|melA]]'' [Pubmed|22383849]
''[[protein|msmE]]-[[protein|amyD]]-[[protein|amyC]]-[[protein|melA]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
''[[protein|msmR]]'': [[protein|SigA]] [Pubmed|22900538]
Expression and Regulation
Regulation
[[protein|MsmR]]
repressed by glucose ([[protein|CcpA]]) [Pubmed|22900538]
Expression and Regulation
Regulatory mechanism
[[protein|CcpA]]: transcription repression [Pubmed|22900538]
References
proteinLength
426
geneLength
1281
Gene
Coordinates
3,097,850 → 3,099,130
The protein
Catalyzed reaction/ biological activity
binding of melibiose and raffinose [pubmed|31138628]
The protein
Protein family
[SW|Bacterial solute-binding protein 1 family] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A801 (msmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/801 NBRP B. subtilis, Japan]
BKE30270 (Δ[[gene|melE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA
BKK30270 (Δ[[gene|melE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT, downstream forward: _UP4_TCCGAAACACAGGGAGCTGA